KEGG   ENZYME: 5.4.99.13Help
Entry
EC 5.4.99.13                Enzyme                                 

Name
isobutyryl-CoA mutase;
isobutyryl coenzyme A mutase;
butyryl-CoA:isobutyryl-CoA mutase;
icmA (gene name);
icmB (gene name);
icmF (gene name)
Class
Isomerases;
Intramolecular transferases;
Transferring other groups
BRITE hierarchy
Sysname
2-methylpropanoyl-CoA CoA-carbonylmutase
Reaction(IUBMB)
2-methylpropanoyl-CoA = butanoyl-CoA [RN:R01181]
Reaction(KEGG)
Substrate
2-methylpropanoyl-CoA [CPD:C00630]
Product
butanoyl-CoA [CPD:C00136]
Comment
This bacterial enzyme utilizes 5'-deoxyadenosylcobalamin as a cofactor. Following substrate binding, the enzyme catalyses the homolytic cleavage of the cobalt-carbon bond of AdoCbl, yielding cob(II)alamin and a 5'-deoxyadenosyl radical, which initiates the the carbon skeleton rearrangement reaction by hydrogen atom abstraction from the substrate. At the end of each catalytic cycle the 5'-deoxyadenosyl radical and cob(II)alamin recombine, regenerating the resting form of the cofactor. The enzyme is prone to inactivation resulting from occassional loss of the 5'-deoxyadenosyl molecule. Inactivated enzymes are repaired by the action of EC 2.5.1.17, cob(I)yrinic acid a,c-diamide adenosyltransferase, and a G-protein chaperone, which restore cob(II)alamin (which is first reduced to cob(I)alamin by an unidentified reductase) to 5'-deoxyadenosylcobalamin and load it back on the mutase. Some mutases are fused with their G-protein chaperone. These enzyme can also catalyse the interconversion of isovaleryl-CoA with pivalyl-CoA.
History
EC 5.4.99.13 created 1992, revised 2017
Orthology
K11942  
isobutyryl-CoA mutase
Genes
XSA: 
XTN: 
SACZ: 
STEK: 
PSU: 
PSUW: 
LAB: 
LAQ: 
LCP: 
LGU: 
LEZ: 
GLE_1980(mutA)
LEM: 
LEN_3025(mcmA)
LUM: 
RHD: 
DJI: 
DJA: 
DTX: 
DKO: 
LRZ: 
COZ: 
COLW: 
MVS: 
CYQ: 
CZA: 
BLEP: 
NOC: 
NHL: 
WMA: 
WOC: 
SDF: 
GBI: 
SEDS: 
PSPI: 
VFF: 
RSO: 
RSC: 
RSL: 
RSN: 
RSM: 
RSE: 
RPI: 
RPF: 
RPJ: 
RMN: 
RIN: 
REU: 
REH: 
H16_A0280(sbm1)
CNC: 
RME: 
CTI: 
CBW: 
CGD: 
CCUP: 
CUP: 
CUU: 
BUG: 
BGE: 
BGF: 
BUQ: 
BXE: 
BXB: 
BPH: 
BPX: 
BPY: 
BCAI: 
PSPW: 
PARA: 
PTX: 
RFR: 
RSB: 
RAC: 
POL: 
PNA: 
RTA: 
LIM: 
LIH: 
HYR: 
HYB: 
HYL: 
CBAA: 
CBAB: 
MPT: 
MNR: 
MASW: 
LCH: 
TIN: 
THI: 
RGE: 
RBN: 
RDP: 
PKT: 
PBH: 
SHD: 
EBA: 
DSU: 
RBU: 
DAR: 
AZO: 
AZA: 
AZI: 
AOA: 
TMZ: 
THU: 
TCL: 
APP: 
GME: 
GUR: 
GBM: 
GEM: 
GEB: 
GPI: 
GAO: 
GSB: 
DES: 
DEU: 
DPR: 
DOL: 
DML: 
DAL: 
DAT: 
DTO: 
AFW: 
MXA: 
MFU: 
MSD: 
MYM: 
CCX: 
SUR: 
AGE: 
MBD: 
CFUS: 
CCRO: 
SAMY: 
LLU: 
MRM: 
DTI: 
DBR: 
BBA: 
BBAT: 
BBW: 
BBAC: 
BEX: 
BMX: 
BHA: 
BPF: 
BMQ: 
BMD: 
BMH: 
BMEG: 
BCO: 
BCK: 
BAG: 
BACI: 
BIF: 
BMET: 
GST: 
BACO: 
BEO: 
BSJ: 
BON: 
BFX: 
BWH: 
BHK: 
BKW: 
GKA: 
GTE: 
GTK: 
GTN: 
GWC: 
GYC: 
GYA: 
GCT: 
GMC: 
GGH: 
GHH_c34870(mutB2)
GJF: 
GEA: 
GARCT_03375(scpA_3)
GEL: 
GSE: 
GSR: 
GEJ: 
GTH: 
PTL: 
AFL: 
AGN: 
ANM: 
AAMY: 
ANL: 
LSP: 
LGY: 
LFU: 
HHD: 
HMN: 
VIR: 
VHL: 
VIL: 
VNE: 
LAO: 
FPN: 
FAR: 
SJE: 
BSE: 
BBE: 
BLR: 
BFM: 
PJD: 
ANX: 
BTS: 
TUM: 
TAB: 
SIV: 
SSIL: 
PLN: 
PKU: 
PRT: 
PANA: 
PDG: 
PHC: 
PMAR: 
PPLA: 
PFAE: 
JEO: 
KUR: 
SPSY: 
SPOR: 
SPOP: 
SURE: 
RST: 
NTR: 
HMO: 
HM1_0899(mutB)
NFA: 
NFR: 
NBR: 
NNO: 
NSR: 
SCB: 
SVL: 
SBH: 
SALS: 
SRC: 
SLS: 
ICA: 
SERJ: 
JTE: 
NCA: 
NDK: 
I601_1647(scpA_1)
PSIM: 
AER: 
TFU: 
TCU: 
SRO: 
NOA: 
FRA: 
FRE: 
FRI: 
FAL: 
FSY: 
GOB: 
MMAR: 
SVI: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
ALU: 
PDX: 
PSEA: 
PSEE: 
PSEH: 
PSEQ: 
PECQ: 
PHH: 
AMI: 
SESP: 
KAL: 
KPHY: 
LED: 
ALL: 
PMAD: 
TBI: 
CWO: 
DPT: 
WCH: 
LIL: 
LIE: 
LIC: 
LIC_11105(mcm1)
LIS: 
LBJ: 
LBI: 
LBF: 
LST: 
LAJ: 
TPX: 
GAU: 
BLQ: 
L21SP5_02883(scpA_2)
SRU: 
SRM: 
SRM_01894(bhbA)
RMR: 
RMG: 
RBAR: 
CPI: 
NKO: 
NSO: 
FLA: 
HHY: 
SGN: 
PHE: 
PEP: 
PCM: 
PSTY: 
SCN: 
MUP: 
MUC: 
BBD: 
EVI: 
ALM: 
SLI: 
SRD: 
RSI: 
FAE: 
FIB: 
FLI: 
HSW: 
HYM: 
HYD: 
HYE: 
HYG: 
HYP: 
HYZ: 
PKO: 
RTI: 
RUD: 
MTT: 
FLM: 
FBT: 
GFO: 
GRL: 
GFL: 
FJO: 
FJG: 
BB050_00315(scpA_1)
FPS: 
FPC: 
FPY: 
FPO: 
FPQ: 
FPV: 
FPW: 
FPK: 
FPSZ: 
FBR: 
FCO: 
FIN: 
FGL: 
FCM: 
RBI: 
ZPR: 
CAT: 
CAO: 
CLY: 
CLH: 
CBAL: 
CBAT: 
WVI: 
KDI: 
DOK: 
DDO: 
I597_2470(scpA_2)
LAN: 
LVN: 
MRS: 
MLT: 
ASL: 
PTQ: 
NDO: 
NOM: 
NSD: 
POM: 
PRN: 
POLA: 
EAO: 
EMN: 
ELB: 
VO54_02353(scpA_2)
EEN: 
EMG: 
EGO: 
EGM: 
MYR: 
MPW: 
MOD: 
MYZ: 
CHZ: 
CGN: 
CIH: 
CHH: 
CIO: 
CHRY: 
CPIP: 
WIN: 
WIJ: 
SZE: 
SYI: 
TDI: 
TEN: 
TJE: 
LUT: 
SALT: 
SEON: 
AALG: 
FBA: 
FBU: 
FTE: 
FLU: 
OHO: 
BBAU: 
 » show all
Taxonomy
Reference
1
  Authors
Brendelberger, G., Retey, J., Ashworth, D.M., Reynolds, K., Willenbrock, F. and Robinson, J.A.
  Title
The enzymic interconversion of isobutyryl and N-butyrylcarba(dethia)-coenzyme-A - a coenzyme-B12-dependent carbon skeleton rearrangement.
  Journal
Angew. Chem. Int. Ed. Engl. 27 (1988) 1089-1091.
Reference
2  [PMID:10531377]
  Authors
Ratnatilleke A, Vrijbloed JW, Robinson JA.
  Title
Cloning and sequencing of the coenzyme B(12)-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli.
  Journal
J. Biol. Chem. 274 (1999) 31679-85.
Reference
3  [PMID:19864421]
  Authors
Cracan V, Padovani D, Banerjee R
  Title
IcmF is a fusion between the radical B12 enzyme isobutyryl-CoA mutase and its G-protein chaperone.
  Journal
J. Biol. Chem. 285 (2010) 655-66.
  Sequence
[gka:GK3391] [nfa:NFA_10360] [bxe:Bxe_A4277]
Reference
4  [PMID:22167181]
  Authors
Cracan V, Banerjee R
  Title
Novel coenzyme B12-dependent interconversion of isovaleryl-CoA and pivalyl-CoA.
  Journal
J. Biol. Chem. 287 (2012) 3723-32.
  Sequence
[rme:Rmet_0210] [gka:GK3391]
Reference
5  [PMID:26318610]
  Authors
Jost M, Born DA, Cracan V, Banerjee R, Drennan CL
  Title
Structural Basis for Substrate Specificity in Adenosylcobalamin-dependent Isobutyryl-CoA Mutase and Related Acyl-CoA Mutases.
  Journal
J. Biol. Chem. 290 (2015) 26882-98.
  Sequence
[rme:Rmet_0210]
Reference
6  [PMID:28130442]
  Authors
Li Z, Kitanishi K, Twahir UT, Cracan V, Chapman D, Warncke K, Banerjee R
  Title
Cofactor Editing by the G-protein Metallochaperone Domain Regulates the Radical B12 Enzyme IcmF.
  Journal
J. Biol. Chem. 292 (2017) 3977-3987.
  Sequence
[rme:Rmet_0210]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
116405-23-3

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