KEGG   ENZYME: 6.1.1.26Help
Entry
EC 6.1.1.26                 Enzyme                                 

Name
pyrrolysine---tRNAPyl ligase;
PylS;
pyrrolysyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy
Sysname
L-pyrrolysine:tRNAPyl ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl [RN:R08578]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-pyrrolysine [CPD:C16138];
tRNA(Pyl) [CPD:C16139]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-pyrrolysyl-tRNA(Pyl) [CPD:C17027]
Comment
In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl---tRNA ligase. The enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids [1].
History
EC 6.1.1.26 created 2007
Pathway
Aminoacyl-tRNA biosynthesis
Orthology
K11627  
pyrrolysyl-tRNA synthetase
Genes
MAC: 
MA0155(pylS)
MBA: 
MMA: 
MM_1445(pylS)
MBU: 
Mbur_2086(pylS)
MMH: 
MEV: 
MZH: 
MPY: 
Mpsy_1721(pylS)
MHZ: 
Taxonomy
Reference
1  [PMID:15329732]
  Authors
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
  Title
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
  Journal
Nature. 431 (2004) 333-5.
  Organism
Methanosarcina barkeri
  Sequence
Reference
2  [PMID:15314242]
  Authors
Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D.
  Title
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 12450-4.
  Organism
Methanosarcina barkeri
  Sequence
Reference
3  [PMID:15372017]
  Authors
Schimmel P, Beebe K.
  Title
Molecular biology: genetic code seizes pyrrolysine.
  Journal
Nature. 431 (2004) 257-8.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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