coenzyme F420-1:gamma-L-glutamate ligase;
F420:gamma-glutamyl ligase;
CofE-AF;
MJ0768;
CofE

Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)

L-glutamate:coenzyme F420-1 ligase (GDP-forming)

GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2 [RN:R09400]

R09400

GTP [CPD:C00044];
coenzyme F420-1 [CPD:C19152];
L-glutamate [CPD:C00025]

This protein catalyses the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the first reaction (EC 6.3.2.31, coenzyme F420-0---L-glutamate ligase) the enzyme attaches a glutamate via its alpha-amine group to F420-0. In the second reaction, which is described here, the enzyme catalyses the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.

1  [PMID:12911320]

Li H, Graupner M, Xu H, White RH

CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.

Biochemistry. 42 (2003) 9771-8.

2  [PMID:17669425]

Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A

Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases.

J. Mol. Biol. 372 (2007) 456-69.