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Entry
EC 6.3.4.22                 Enzyme                                 

Name
tRNAIle2-agmatinylcytidine synthase;
TiaS;
AF2259;
tRNAIle-2-agmatinylcytidine synthetase;
tRNAIle-agm2C synthetase;
tRNAIle-agmatidine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Other carbon-nitrogen ligases
BRITE hierarchy
Sysname
agmatine:[tRNAIle]-cytidine34 ligase
Reaction(IUBMB)
ATP + agmatine + [tRNAIle2]-cytidine34 + H2O = [tRNAIle2]-2-agmatinylcytidine34 + AMP + 2 phosphate [RN:R10496]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
agmatine [CPD:C00179];
[tRNAIle2]-cytidine34;
H2O [CPD:C00001]
Product
[tRNAIle2]-2-agmatinylcytidine34;
AMP [CPD:C00020];
phosphate [CPD:C00009]
Comment
The enzyme from the archaeon Archaeoglobus fulgidus modifies the wobble base of the CAU anticodon of the archaeal tRNAIle2 at the oxo group in position 2 of cytidine34. This modification is crucial for accurate decoding of the genetic code. In bacteria EC 6.3.4.19, tRNAIle-lysidine synthase, catalyses the modification of [tRNAIle2]-cytidine34 to [tRNAIle2]-lysidine34 .
History
EC 6.3.4.22 created 2013
Orthology
K06932  tRNA(Ile2)-agmatinylcytidine synthase
Genes
MJA: MJ_1095
MFE: Mefer_1336
MVU: Metvu_1118
MFS: MFS40622_0162
MIF: Metin_0603
MJH: JH146_0033
MIG: Metig_0478
MMP: MMP0616
MMQ: MmarC5_0989
MMX: MmarC6_0275
MMD: GYY_03390
MAE: Maeo_0755
MVO: Mvol_0248
MAC: MA_3525
MBAR: MSBR2_1401
MBAK: MSBR3_1473
MMA: MM_0445
MMAC: MSMAC_0847
METM: MSMTP_2364
MTHE: MSTHC_2224
MTHR: MSTHT_1079
MHOR: MSHOH_3068
MBU: Mbur_1810
MMET: MCMEM_0061
MMH: Mmah_1918
MPY: Mpsy_1823
MTP: Mthe_0611
MCJ: MCON_1904
MHI: Mhar_0187
MHU: Mhun_2547
MLA: Mlab_0159
MEMA: MMAB1_3347(tiaS)
MPI: Mpet_0081
MBN: Mboo_0194
MPL: Mpal_0030
MPD: MCP_2871
MEZ: Mtc_0281
RCI: RCIX1429
MTH: MTH_966
METC: MTCT_0877
MWO: MWSIV6_1356(tiaS)
METE: tca_00929
MST: Msp_1142
MSI: Msm_0452
MRU: mru_0685
MEB: Abm4_0413
MMIL: sm9_0558
MEYE: TL18_02620
MOL: YLM1_0329
METH: MBMB1_1591(tiaS)
MFC: BRM9_0826
MFI: DSM1535_0789(tiaS)
MCUB: MCBB_1889(tiaS)
MFV: Mfer_0668
MKA: MK1003
AFU: AF_2259
FPL: Ferp_1288
GAC: GACE_1471
GAH: GAH_01052
HAL: VNG_2110C
HSL: OE_3943R(tiaS)
HHB: Hhub_3400(tiaS)
HALH: HTSR_1874
HHSR: HSR6_1944(tiaS)
HMA: rrnAC2518
HHI: HAH_2948
NPH: NP_1318A(tiaS)
NMO: Nmlp_3253(tiaS)
HUT: Huta_1790
HTI: HTIA_1320
HMU: Hmuk_0845
HWA: HQ_3056A(tiaS)
HWC: Hqrw_3574(tiaS)
HVO: HVO_0339(tiaS)
HME: HFX_0324
HLA: Hlac_0272
HTU: Htur_2426
NMG: Nmag_1178
NAT: NJ7G_1333
SALI: L593_15020
TAC: Ta0545
TVO: TVG0590669(TVG0590669)
PTO: PTO1377
FAI: FAD_0572(tiaS)
CDIV: CPM_0360
MEAR: Mpt1_c14580(tiaS2)
MARC: AR505_0003
PHO: PH1812(PH1812)
PAB: PAB0247
PFU: PF1855
PFI: PFC_09315
PYN: PNA2_0411
PYS: Py04_1738
TKO: TK0553
TON: TON_0699
TGA: TGAM_1029
TSI: TSIB_1197
THE: GQS_01425
THA: TAM4_479
THM: CL1_2002
TLT: OCC_01114
THS: TES1_0950
TNU: BD01_0657
TEU: TEU_08925
PPAC: PAP_07755
ABI: Aboo_0928
ACJ: ACAM_0488
SMR: Smar_1362
IHO: Igni_0309
IIS: EYM_07450
DKA: DKAM_0469
TAG: Tagg_0667
IAG: Igag_0055
HBU: Hbut_0008
SSO: SSO0258
SOL: Ssol_1234
SSOA: SULA_1275
SSOL: SULB_1276
SSOF: SULC_1274
STO: STK_03120
SAI: Saci_0732
SID: M164_1889
SII: LD85_2103
SIH: SiH_1819
SIR: SiRe_1739
SIC: SiL_1732
MSE: Msed_2296
MCN: Mcup_2039
AHO: Ahos_0781
PAI: PAE0933
PIS: Pisl_1170
PCL: Pcal_0100
PAS: Pars_0133
PYR: P186_2378
POG: Pogu_2352
TNE: Tneu_0126
CMA: Cmaq_0160
TUZ: TUZN_0192
TTN: TTX_0548
VDI: Vdis_1829
VMO: VMUT_0270
TPE: Tpen_0604
ASC: ASAC_1057
NMR: Nmar_1562
NID: NPIRD3C_0493(tiaS)
NCT: NMSP_0197(tiaS)
NEV: NTE_00071
NCV: NCAV_0167(tiaS)
NBV: T478_1284
NDV: NDEV_1925(tiaS)
BARC: AOA65_1257(tiaS_1) AOA65_1587(tiaS_2)
BARB: AOA66_1422(tiaS)
 » show all
Taxonomy
Reference
1  [PMID:20139989]
  Authors
Ikeuchi Y, Kimura S, Numata T, Nakamura D, Yokogawa T, Ogata T, Wada T, Suzuki T, Suzuki T
  Title
Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.
  Journal
Nat. Chem. Biol. 6 (2010) 277-82.
  Sequence
[afu:AF_2259] [pho:PH1812]
Reference
2  [PMID:22002222]
  Authors
Terasaka N, Kimura S, Osawa T, Numata T, Suzuki T
  Title
Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS.
  Journal
Nat. Struct. Mol. Biol. 18 (2011) 1268-74.
Reference
3  [PMID:22102245]
  Authors
Osawa T, Inanaga H, Kimura S, Terasaka N, Suzuki T, Numata T
  Title
Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA-modification enzyme, TiaS, complexed with tRNA(Ile2) and ATP.
  Journal
Acta. Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 67 (2011) 1414-6.
Other DBs
ExplorEnz - The Enzyme Database: 6.3.4.22
IUBMB Enzyme Nomenclature: 6.3.4.22
ExPASy - ENZYME nomenclature database: 6.3.4.22
BRENDA, the Enzyme Database: 6.3.4.22

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