KEGG   ENZYME: 1.1.99.28Help
Entry
EC 1.1.99.28                Enzyme                                 
Name
glucose-fructose oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With other acceptors
BRITE hierarchy
Sysname
D-glucose:D-fructose oxidoreductase
Reaction(IUBMB)
D-glucose + D-fructose = D-gluconolactone + D-glucitol [RN:R00874]
Reaction(KEGG)
Substrate
D-glucose [CPD:C00031];
D-fructose [CPD:C00095]
Product
D-gluconolactone [CPD:C00198];
D-glucitol [CPD:C00794]
Comment
D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose will function as aldose substrates, but with low affinities. The ketose substrate must be in the open-chain form. The apparent affinity for fructose is low, because little of the fructose substrate is in the open-chain form. Xylulose and glycerone (dihydroxyacetone) will replace fructose, but they are poor substrates. The enzyme from Zymomonas mobilis contains tightly bound NADP+.
Orthology
K00118  
glucose-fructose oxidoreductase
Genes
MGR: 
AFM: 
XCC: 
XCC0815(gfo)
XCB: 
XCA: 
XCV: 
XCV0923(gfo)
XCP: 
XAC: 
XAC0888(gfo)
XAX: 
XOO: 
XOO3722(gfo)
XOM: 
XOO_3515
XOP: 
PXO_04621
XOR: 
XOC_0940
XAL: 
XALc_2749
PSU: 
Psesu_0603
CJA: 
CJA_2424(gfo)
AMC: 
MADE_1016150
AMAC: 
MASE_15230
AMB: 
AMBAS45_15740
AMG: 
AMEC673_15610
AMK: 
AMBLS11_15060
AMAA: 
amad1_16280
ALT: 
ambt_01340
TTU: 
TERTU_4571
RSO: 
RS02580(RSp1076)
VEI: 
Veis_3569
BME: 
BMEI0020
BMF: 
BAB1_2049
CCR: 
CC_1225
ZMO: 
ZMO0689
ZMN: 
Za10_0578
RHA: 
RHA1_ro02475
KRH: 
KRH_21840(gfo)
SRU: 
SRU_0850(gfo)
SRM: 
SRM_01033(gfo)
NKO: 
Niako_4178
HHY: 
Halhy_3652
CMR: 
Cycma_2931
EVI: 
Echvi_2803
DFE: 
Dfer_1598
SLI: 
Slin_2554
RSI: 
Runsl_5400
EOL: 
Emtol_3209
FAE: 
FAES_2065
GFO: 
GFO_2195(gfo)
RBI: 
RB2501_15414
ZGA: 
zobellia_4647(gfoA)
MRS: 
Murru_3045
OTE: 
Oter_0724
RBA: 
RB3265(gfo)
NPU: 
Npun_R3642
DTH: 
DICTH_0475
DTU: 
Dtur_0639
 » show all
Taxonomy
Reference
1  [PMID:3745122]
  Authors
Zachariou M, Scopes RK.
  Title
Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production.
  Journal
J. Bacteriol. 167 (1986) 863-9.
  Organism
Zymomonas mobilis [GN:zmo]
Reference
2  [PMID:3356190]
  Authors
Hardman MJ, Scopes RK.
  Title
The kinetics of glucose-fructose oxidoreductase from Zymomonas mobilis.
  Journal
Eur. J. Biochem. 173 (1988) 203-9.
  Organism
Zymomonas mobilis [GN:zmo]
Reference
3  [PMID:1537789]
  Authors
Kanagasundaram V, Scopes RK.
  Title
Cloning, sequence analysis, and expression of the structural gene encoding glucose-fructose oxidoreductase from Zymomonas mobilis.
  Journal
J. Bacteriol. 174 (1992) 1439-47.
  Organism
Zymomonas mobilis [GN:zmo]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
94949-35-6
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