KEGG   ENZYME: 1.1.99.28Help
Entry
EC 1.1.99.28                Enzyme                                 

Name
glucose-fructose oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With other, unknown, acceptors
BRITE hierarchy
Sysname
D-glucose:D-fructose oxidoreductase
Reaction(IUBMB)
D-glucose + D-fructose = D-gluconolactone + D-glucitol [RN:R00874]
Reaction(KEGG)
Substrate
D-glucose [CPD:C00031];
D-fructose [CPD:C00095]
Product
D-gluconolactone [CPD:C00198];
D-glucitol [CPD:C00794]
Comment
D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose will function as aldose substrates, but with low affinities. The ketose substrate must be in the open-chain form. The apparent affinity for fructose is low, because little of the fructose substrate is in the open-chain form. Xylulose and glycerone (dihydroxyacetone) will replace fructose, but they are poor substrates. The enzyme from Zymomonas mobilis contains tightly bound NADP+.
History
EC 1.1.99.28 created 1999
Orthology
K00118  
glucose-fructose oxidoreductase
Genes
MGR: 
AFM: 
XCC: 
XCC0815(gfo)
XCB: 
XCA: 
XCP: 
XCV: 
XCV0923(gfo)
XAC: 
XAC0888(gfo)
XAX: 
XOO: 
XOO3722(gfo)
XOM: 
XOP: 
XOR: 
XAL: 
PSU: 
CJA: 
AMC: 
AMAC: 
AMB: 
AMG: 
AMK: 
AMAA: 
ALT: 
TTU: 
RSO: 
RS02580(RSp1076)
VEI: 
BME: 
BMF: 
CCR: 
ZMO: 
ZMN: 
RHA: 
KRH: 
OTE: 
RBA: 
RB3265(gfo)
NPU: 
SRU: 
SRM: 
NKO: 
HHY: 
CMR: 
EVI: 
DFE: 
SLI: 
RSI: 
EOL: 
FAE: 
GFO: 
RBI: 
ZGA: 
MRS: 
DTH: 
DTU: 
 » show all
Taxonomy
Reference
1  [PMID:3745122]
  Authors
Zachariou M, Scopes RK.
  Title
Glucose-fructose oxidoreductase, a new enzyme isolated from Zymomonas mobilis that is responsible for sorbitol production.
  Journal
J. Bacteriol. 167 (1986) 863-9.
Reference
2  [PMID:3356190]
  Authors
Hardman MJ, Scopes RK.
  Title
The kinetics of glucose-fructose oxidoreductase from Zymomonas mobilis.
  Journal
Eur. J. Biochem. 173 (1988) 203-9.
Reference
3  [PMID:1537789]
  Authors
Kanagasundaram V, Scopes RK.
  Title
Cloning, sequence analysis, and expression of the structural gene encoding glucose-fructose oxidoreductase from Zymomonas mobilis.
  Journal
J. Bacteriol. 174 (1992) 1439-47.
  Sequence
[zmo:ZMO0689]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
94949-35-6

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