Photinus-luciferin 4-monooxygenase (ATP-hydrolysing);
firefly luciferase;
luciferase (firefly luciferin);
Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing);
firefly luciferin luciferase;
Photinus pyralis luciferase

Oxidoreductases;
Acting on single donors with O2 as oxidant and incorporation of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O2;
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)

Photinus-luciferin:oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolysing)

Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + hnu [RN:R04036]

R04036

Photinus luciferin [CPD:C02740];
O2 [CPD:C00007];
ATP [CPD:C00002]

Photinus (firefly) is a bioluminescent insect. The first step in the reaction is the formation of an acid anhydride between the carboxylic group and AMP, with the release of diphosphate. The enzyme may be assayed by measurement of light emission.

1  [PMID:6064360]

Hopkins TA, Seliger HH, White EH, Cass MH.

The chemiluminescence of firefly luciferin. A model for the bioluminescent reaction and identification of the product excited state.

J. Am. Chem. Soc. 89 (1967) 7148-50.

Photinus pyralis

2

White, E.H., McCapra, F., Field, G.F. and McElroy, W.D.

The structure and synthesis of firefly luciferin.

J. Am. Chem. Soc. 83 (1961) 2402-2403.

3  [PMID:5784183]

White EH, Rapaport E, Hopkins TA, Seliger HH.

Chemi- and bioluminescence of firefly luciferin.

J. Am. Chem. Soc. 91 (1969) 2178-80.

Photinus pyralis