ec:1.14.11.n4 : No such data.

KEGG   ENZYME: 1.14.11.30Help
Entry
EC 1.14.11.30               Enzyme                                 

Name
hypoxia-inducible factor-asparagine dioxygenase;
HIF hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
hypoxia-inducible factor-L-asparagine, 2-oxoglutarate:oxygen oxidoreductase (4-hydroxylating)
Reaction(IUBMB)
hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O2 = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO2
Substrate
hypoxia-inducible factor-L-asparagine;
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine;
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Contains iron, and requires ascorbate. Catalyses hydroxylation of an asparagine in the C-terminal transcriptional activation domain of HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which reduces its interaction with the transcriptional coactivator protein p300. The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.
History
EC 1.14.11.30 created 2010
Orthology
K18055  
hypoxia-inducible factor 1-alpha inhibitor (HIF hydroxylase)
Genes
HSA: 
55662(HIF1AN)
PTR: 
466183(HIF1AN)
PPS: 
100969925(HIF1AN)
GGO: 
101140559(HIF1AN)
PON: 
100450996(HIF1AN)
NLE: 
100604492(HIF1AN)
MCC: 
710425(HIF1AN)
MCF: 
102123484(HIF1AN)
CJC: 
100415279(HIF1AN)
MMU: 
319594(Hif1an)
RNO: 
309434(Hif1an)
CGE: 
100752342(Hif1an)
HGL: 
101715790(Hif1an)
TUP: 
102471470(HIF1AN)
CFA: 
609221(HIF1AN)
AML: 
100484876(HIF1AN)
UMR: 
103677605(HIF1AN)
FCA: 
101091901(HIF1AN)
PTG: 
102952057(HIF1AN)
BTA: 
520864(HIF1AN)
BOM: 
102285023(HIF1AN)
PHD: 
102341833(HIF1AN)
CHX: 
102182662(HIF1AN)
OAS: 
101122066(HIF1AN)
SSC: 
CFR: 
102504253(HIF1AN)
BACU: 
103010817(HIF1AN)
LVE: 
103087616(HIF1AN)
ECB: 
100070337(HIF1AN)
MYB: 
102258997(HIF1AN)
MYD: 
102758329(HIF1AN)
PALE: 
102898816(HIF1AN)
MDO: 
100010508(HIF1AN)
SHR: 
100932046(HIF1AN)
OAA: 
100084505(HIF1AN)
GGA: 
428952(HIF1AN)
MGP: 
100547017(HIF1AN)
APLA: 
101796702(HIF1AN)
TGU: 
100224185(HIF1AN)
FAB: 
101815471(HIF1AN)
PHI: 
102110633(HIF1AN)
FPG: 
101910571(HIF1AN)
FCH: 
102050862(HIF1AN)
CLV: 
102094216(HIF1AN)
ASN: 
102372847(HIF1AN)
AMJ: 
102557843(HIF1AN)
PSS: 
102461331(HIF1AN)
CMY: 
102940361(HIF1AN)
ACS: 
100564872(hif1an)
PBI: 
103049844(HIF1AN)
XLA: 
432095(hif1an)
XTR: 
394511(hif1an)
DRE: 
373126(hif1an)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102346962(HIF1AN)
CMK: 
103182440(hif1an)
BFO: 
CIN: 
TCA: 
664369(HIF)
API: 
PHU: 
ISC: 
HRO: 
LGI: 
NVE: 
AQU: 
CSL: 
EHX: 
GTT: 
HAO: 
GLP: 
 » show all
Taxonomy
Reference
1  [PMID:11641274]
  Authors
Mahon PC, Hirota K, Semenza GL
  Title
FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity.
  Journal
Genes. Dev. 15 (2001) 2675-86.
  Sequence
[hsa:55662]
Reference
2  [PMID:12042299]
  Authors
Hewitson KS, McNeill LA, Riordan MV, Tian YM, Bullock AN, Welford RW, Elkins JM, Oldham NJ, Bhattacharya S, Gleadle JM, Ratcliffe PJ, Pugh CW, Schofield CJ
  Title
Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical to factor inhibiting HIF (FIH) and is related to the cupin structural family.
  Journal
J. Biol. Chem. 277 (2002) 26351-5.
  Sequence
[hsa:55662]
Reference
3  [PMID:12432100]
  Authors
Dann CE 3rd, Bruick RK, Deisenhofer J
  Title
Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 15351-6.
  Sequence
[hsa:55662]
Reference
4  [PMID:11823643]
  Authors
Lando D, Peet DJ, Whelan DA, Gorman JJ, Whitelaw ML
  Title
Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch.
  Journal
Science. 295 (2002) 858-61.
  Sequence
[hsa:55662]
Reference
5  [PMID:14701857]
  Authors
Koivunen P, Hirsila M, Gunzler V, Kivirikko KI, Myllyharju J
  Title
Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases.
  Journal
J. Biol. Chem. 279 (2004) 9899-904.
  Sequence
[hsa:55662]
Reference
6  [PMID:12446723]
  Authors
Elkins JM, Hewitson KS, McNeill LA, Seibel JF, Schlemminger I, Pugh CW, Ratcliffe PJ, Schofield CJ
  Title
Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha.
  Journal
J. Biol. Chem. 278 (2003) 1802-6.
  Sequence
[hsa:55662]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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