KEGG   ENZYME: 1.14.13.39Help
Entry
EC 1.14.13.39               Enzyme                                 

Name nitric-oxide synthase;
nitric oxide synthetase;
endothelium-derived relaxation factor-forming enzyme;
endothelium-derived relaxing factor synthase;
NO synthase;
NADPH-diaphorase
Class Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
BRITE hierarchy
Sysname L-arginine,NADPH:oxygen oxidoreductase (nitric-oxide-forming)
Reaction(IUBMB) L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n
NADP+ [RN:R00557]
Reaction(KEGG) R00557;
(other) R00111 R00558
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Substrate L-arginine [CPD:C00062];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product citrulline [CPD:C00327];
nitric oxide [CPD:C00533];
NADP+ [CPD:C00006]
Cofactor Calcium [CPD:C00076]
Inhibitor Ngamma-Nitro-L-arginine [CPD:C03417];
Ngamma-Monomethyl-L-arginine [CPD:C03884];
Ngamma-Nitro-L-arginine methyl ester [CPD:C04337]
Comment The enzyme in brain, but not that induced in lung or liver by
endotoxin, requires Ca2+. The stoichiometry is not clear, but may
involve a two-electron and a one-electron oxidation step.
Pathway PATH: ec00330  Arginine and proline metabolism
PATH: ec01100  Metabolic pathways
Orthology KO: K00491  nitric-oxide synthase, bacterial
KO: K13240  nitric-oxide synthase, brain
KO: K13241  nitric-oxide synthase, inducible
KO: K13242  nitric-oxide synthase, endothelial
KO: K13253  nitric-oxide synthase, invertebrate
Genes HSA: 4842(NOS1) 4843(NOS2) 4846(NOS3)
PTR: 455026(NOS2)
MCC: 693781(NOS1) 706921(NOS2)
MMU: 18125(Nos1) 18126(Nos2) 18127(Nos3)
RNO: 24598(Nos1) 24599(Nos2) 24600(Nos3)
CFA: 403784(NOS3) 403822(NOS2) 477498(NOS1)
BTA: 282876(NOS2) 287024(NOS3) 536132(NOS1)
SSC: 396859(NOS2) 397557(NOS3)
ECB: 100051079(NOS1) 100063339 791246(INOS)
MDO: 100013712 100018035 100024271
OAA: 100075386 100079842 100091599
GGA: 395807(NOS2) 427721(NOS1)
TGU: 100230954 100231414
XLA: 373705(nos1-A)
DRE: 404036(nos2a) 563654(nos2b) 60658(nos1)
BFO: BRAFLDRAFT_116914 BRAFLDRAFT_120590 BRAFLDRAFT_290770(NosC)
CIN: 100183039
SPU: 587111
DME: Dmel_CG6713(Nos)
DPO: Dpse_GA19805
DAN: Dana_GF14065
DER: Dere_GG10354
DPE: Dper_GL26383
DSE: Dsec_GM11413
DSI: Dsim_GD22223
DYA: Dyak_GE13295
DGR: Dgri_GH13337
DMO: Dmoj_GI17812
AGA: AgaP_AGAP008255 AgaP_AGAP008257
AAG: AaeL_AAEL009745
CQU: CpipJ_CPIJ000366
AME: 503861(NOS)
NVI: 100119281
TCA: 655549
API: 100160390
ISC: IscW_ISCW018074
NVE: NEMVE_v1g110599
HMG: 100215023
TAD: TRIADDRAFT_18893 TRIADDRAFT_31504
OLU: OSTLU_37787
AOR: AO090138000170
ANG: An15g07930
SCL: sce1582(nos)
BSU: BSU07630(nosA)
BHA: BH0823
BAN: BA5695
BAR: GBAA5695
BAA: BA_0553
BAT: BAS5299
BAH: BAMEG_5743(nos)
BAI: BAA_5726(nos)
BCE: BC5444
BCA: BCE_5578
BCZ: BCZK5141(nos)
BCR: BCAH187_A5626(nos)
BCB: BCB4264_A5570
BCU: BCAH820_5540
BCG: BCG9842_B5379(nos)
BCQ: BCQ_5288(nos)
BCX: BCA_5597(nos)
BCY: Bcer98_3961
BTK: BT9727_5126(nos)
BTL: BALH_4953(nos)
BWE: BcerKBAB4_5238
BLI: BL03064(nos)
BLD: BLi00785(yflM)
BCL: ABC1267
BAY: RBAM_007830(yflM)
BPU: BPUM_0714(nos)
OIH: OB2691
GKA: GK1676
GYM: GYMC10_4638
GYC: GYMC61_2511
AFL: Aflv_0298
SAU: SA1730
SAV: SAV1914
SAW: SAHV_1899
SAM: MW1855
SAR: SAR2007
SAS: SAS1838
SAC: SACOL1976
SAB: SAB1851
SAA: SAUSA300_1895
SAX: USA300HOU_1916
SAO: SAOUHSC_02134
SAJ: SaurJH9_1970
SAH: SaurJH1_2004
SAE: NWMN_1852
SAD: SAAV_1981
SEP: SE1598
SER: SERP1451
SHA: SH1038
SSP: SSP0877
SCA: Sca_1487(nos)
LSP: Bsph_0728
ESI: Exig_0344
EAT: EAT1b_1019
MCL: MCCL_1651
BBE: BBR47_57280(nos)
PJD: Pjdr2_3338
AAC: Aaci_2338
RHA: RHA1_ro02793(nos)
RER: RER_13870(nos)
ROP: ROP_24100(nos)
SMA: SAV_1531(nos)
SRO: Sros_2358
NML: Namu_4253
SEN: SACE_5845(nos) SACE_7255(nos)
AMI: Amir_1303
SAQ: Sare_3563
CAI: Caci_1733
DRA: DR_2597
DGE: Dgeo_0268
DDR: Deide_13130
NPH: NP1908A(nos)
Taxonomy
Structures PDB: 1D0C  1D0O  1D1V  1D1W  1D1X  1D1Y  1DD7  1DF1  1DM6  1DM7  
     1DM8  1DMI  1DMJ  1DMK  1DWV  1DWW  1DWX  1ED4  1ED5  1ED6  
     1F20  1FOI  1FOJ  1FOL  1FOO  1FOP  1I83  1JWJ  1JWK  1K2R  
     1K2S  1K2T  1K2U  1LZX  1LZZ  1M00  1M7V  1M7Z  1M8D  1M8E  
     1M8H  1M8I  1M9J  1M9K  1M9M  1M9Q  1M9R  1M9T  1MJT  1MMV  
     1MMW  1N2N  1NIW  1NOC  1NOD  1NOS  1NSE  1NSI  1OM4  1OM5  
     1P6H  1P6I  1P6J  1P6K  1P6L  1P6M  1P6N  1Q2O  1QOM  1QW4  
     1QW5  1QW6  1QWC  1R35  1RS6  1RS7  1RS8  1RS9  1TLL  1VAF  
     1VAG  1ZVI  1ZVL  1ZZQ  1ZZR  1ZZS  1ZZT  1ZZU  2AMO  2AN0  
     2AN2  2BHJ  2FC1  2FC2  2FLQ  2G6H  2G6I  2G6J  2G6K  2G6L  
     2G6M  2G6N  2G6O  2HX2  2HX3  2HX4  2NOD  2NOS  2NSE  2NSI  
     2ORO  2ORP  2ORQ  2ORR  2ORS  2ORT  3B3M  3B3N  3B3O  3B3P  
     3DQR  3DQS  3DQT  3DWJ  3E65  3E67  3E68  3E6L  3E6N  3E6O  
     3E6T  3E7G  3E7I  3E7M  3E7S  3E7T  3EAH  3EAI  3EBD  3EBF  
     3EJ8  3FC5  3HR4  3HSN  3HSO  3HSP  3JT3  3JT4  3JT5  3JT6  
     3JT7  3JT8  3JT9  3JTA  3NOD  3NOS  3NSE  4NOS  4NSE  5NSE  
     6NSE  7NSE  8NSE  9NSE  
Reference
  Authors
  Title
  Journal
  Organism
1  [PMID:1689048]
Bredt DS, Snyder SH.
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
Proc. Natl. Acad. Sci. U. S. A. 87 (1990) 682-5.
Rattus norvegicus [GN:rno]
Reference
  Authors
  Title

  Journal
  Organism
2  [PMID:1700698]
Knowles RG, Merrett M, Salter M, Moncada S.
Differential induction of brain, lung and liver nitric oxide
synthase by endotoxin in the rat.
Biochem. J. 270 (1990) 833-6.
Rattus norvegicus [GN:rno]
Reference
  Authors
  Title

  Journal
  Organism
3  [PMID:2567594]
Moncada S, Palmer RM, Higgs EA.
Biosynthesis of nitric oxide from L-arginine. A pathway for the
regulation of cell function and communication.
Biochem. Pharmacol. 38 (1989) 1709-15.
Homo sapiens [GN:hsa]
Other DBs ExplorEnz - The Enzyme Database: 1.14.13.39
IUBMB Enzyme Nomenclature: 1.14.13.39
ExPASy - ENZYME nomenclature database: 1.14.13.39
BRENDA, the Enzyme Database: 1.14.13.39
CAS: 125978-95-2

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