| Entry |
|
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| Name |
nitric-oxide synthase;
nitric oxide synthetase;
endothelium-derived relaxation factor-forming enzyme;
endothelium-derived relaxing factor synthase;
NO synthase;
NADPH-diaphorase |
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| Class |
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or
reduction of oxygen. The oxygen incorporated need not be derived
from O2;
With NADH or NADPH as one donor, and incorporation of one atom of
oxygen into the other donor
 |
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| Sysname |
L-arginine,NADPH:oxygen oxidoreductase (nitric-oxide-forming) |
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| Reaction(IUBMB) |
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n
NADP+ [RN:R00557] |
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| Reaction(KEGG) |
R00557;
(other) R00111 R00558
 |
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| Substrate |
L-arginine [CPD:C00062];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007] |
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| Product |
citrulline [CPD:C00327];
nitric oxide [CPD:C00533];
NADP+ [CPD:C00006] |
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| Cofactor |
Calcium [CPD:C00076] |
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| Inhibitor |
Ngamma-Nitro-L-arginine [CPD:C03417];
Ngamma-Monomethyl-L-arginine [CPD:C03884];
Ngamma-Nitro-L-arginine methyl ester [CPD:C04337] |
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| Comment |
The enzyme in brain, but not that induced in lung or liver by
endotoxin, requires Ca2+. The stoichiometry is not clear, but may
involve a two-electron and a one-electron oxidation step. |
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| Pathway |
PATH: ec00330 Arginine and proline metabolism
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K00491 nitric-oxide synthase, bacterial
KO: K13240 nitric-oxide synthase, brain
KO: K13241 nitric-oxide synthase, inducible
KO: K13242 nitric-oxide synthase, endothelial
KO: K13253 nitric-oxide synthase, invertebrate |
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| Genes |
HSA: 4842(NOS1) 4843(NOS2) 4846(NOS3)
PTR: 455026(NOS2)
MCC: 693781(NOS1) 706921(NOS2)
MMU: 18125(Nos1) 18126(Nos2) 18127(Nos3)
RNO: 24598(Nos1) 24599(Nos2) 24600(Nos3)
CFA: 403784(NOS3) 403822(NOS2) 477498(NOS1)
BTA: 282876(NOS2) 287024(NOS3) 536132(NOS1)
SSC: 396859(NOS2) 397557(NOS3)
ECB: 100051079(NOS1) 100063339 791246(INOS)
MDO: 100013712 100018035 100024271
OAA: 100075386 100079842 100091599
GGA: 395807(NOS2) 427721(NOS1)
TGU: 100230954 100231414
XLA: 373705(nos1-A)
DRE: 404036(nos2a) 563654(nos2b) 60658(nos1)
BFO: BRAFLDRAFT_116914 BRAFLDRAFT_120590 BRAFLDRAFT_290770(NosC)
CIN: 100183039
SPU: 587111
DME: Dmel_CG6713(Nos)
DPO: Dpse_GA19805
DAN: Dana_GF14065
DER: Dere_GG10354
DPE: Dper_GL26383
DSE: Dsec_GM11413
DSI: Dsim_GD22223
DYA: Dyak_GE13295
DGR: Dgri_GH13337
DMO: Dmoj_GI17812
AGA: AgaP_AGAP008255 AgaP_AGAP008257
AAG: AaeL_AAEL009745
CQU: CpipJ_CPIJ000366
AME: 503861(NOS)
NVI: 100119281
TCA: 655549
API: 100160390
ISC: IscW_ISCW018074
NVE: NEMVE_v1g110599
HMG: 100215023
TAD: TRIADDRAFT_18893 TRIADDRAFT_31504
OLU: OSTLU_37787
AOR: AO090138000170
ANG: An15g07930
SCL: sce1582(nos)
BSU: BSU07630(nosA)
BHA: BH0823
BAN: BA5695
BAR: GBAA5695
BAA: BA_0553
BAT: BAS5299
BAH: BAMEG_5743(nos)
BAI: BAA_5726(nos)
BCE: BC5444
BCA: BCE_5578
BCZ: BCZK5141(nos)
BCR: BCAH187_A5626(nos)
BCB: BCB4264_A5570
BCU: BCAH820_5540
BCG: BCG9842_B5379(nos)
BCQ: BCQ_5288(nos)
BCX: BCA_5597(nos)
BCY: Bcer98_3961
BTK: BT9727_5126(nos)
BTL: BALH_4953(nos)
BWE: BcerKBAB4_5238
BLI: BL03064(nos)
BLD: BLi00785(yflM)
BCL: ABC1267
BAY: RBAM_007830(yflM)
BPU: BPUM_0714(nos)
OIH: OB2691
GKA: GK1676
GYM: GYMC10_4638
GYC: GYMC61_2511
AFL: Aflv_0298
SAU: SA1730
SAV: SAV1914
SAW: SAHV_1899
SAM: MW1855
SAR: SAR2007
SAS: SAS1838
SAC: SACOL1976
SAB: SAB1851
SAA: SAUSA300_1895
SAX: USA300HOU_1916
SAO: SAOUHSC_02134
SAJ: SaurJH9_1970
SAH: SaurJH1_2004
SAE: NWMN_1852
SAD: SAAV_1981
SEP: SE1598
SER: SERP1451
SHA: SH1038
SSP: SSP0877
SCA: Sca_1487(nos)
LSP: Bsph_0728
ESI: Exig_0344
EAT: EAT1b_1019
MCL: MCCL_1651
BBE: BBR47_57280(nos)
PJD: Pjdr2_3338
AAC: Aaci_2338
RHA: RHA1_ro02793(nos)
RER: RER_13870(nos)
ROP: ROP_24100(nos)
SMA: SAV_1531(nos)
SRO: Sros_2358
NML: Namu_4253
SEN: SACE_5845(nos) SACE_7255(nos)
AMI: Amir_1303
SAQ: Sare_3563
CAI: Caci_1733
DRA: DR_2597
DGE: Dgeo_0268
DDR: Deide_13130
NPH: NP1908A(nos)
 |
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| Structures |
PDB: 1D0C 1D0O 1D1V 1D1W 1D1X 1D1Y 1DD7 1DF1 1DM6 1DM7
1DM8 1DMI 1DMJ 1DMK 1DWV 1DWW 1DWX 1ED4 1ED5 1ED6
1F20 1FOI 1FOJ 1FOL 1FOO 1FOP 1I83 1JWJ 1JWK 1K2R
1K2S 1K2T 1K2U 1LZX 1LZZ 1M00 1M7V 1M7Z 1M8D 1M8E
1M8H 1M8I 1M9J 1M9K 1M9M 1M9Q 1M9R 1M9T 1MJT 1MMV
1MMW 1N2N 1NIW 1NOC 1NOD 1NOS 1NSE 1NSI 1OM4 1OM5
1P6H 1P6I 1P6J 1P6K 1P6L 1P6M 1P6N 1Q2O 1QOM 1QW4
1QW5 1QW6 1QWC 1R35 1RS6 1RS7 1RS8 1RS9 1TLL 1VAF
1VAG 1ZVI 1ZVL 1ZZQ 1ZZR 1ZZS 1ZZT 1ZZU 2AMO 2AN0
2AN2 2BHJ 2FC1 2FC2 2FLQ 2G6H 2G6I 2G6J 2G6K 2G6L
2G6M 2G6N 2G6O 2HX2 2HX3 2HX4 2NOD 2NOS 2NSE 2NSI
2ORO 2ORP 2ORQ 2ORR 2ORS 2ORT 3B3M 3B3N 3B3O 3B3P
3DQR 3DQS 3DQT 3DWJ 3E65 3E67 3E68 3E6L 3E6N 3E6O
3E6T 3E7G 3E7I 3E7M 3E7S 3E7T 3EAH 3EAI 3EBD 3EBF
3EJ8 3FC5 3HR4 3HSN 3HSO 3HSP 3JT3 3JT4 3JT5 3JT6
3JT7 3JT8 3JT9 3JTA 3NOD 3NOS 3NSE 4NOS 4NSE 5NSE
6NSE 7NSE 8NSE 9NSE |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:1689048]
Bredt DS, Snyder SH.
Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme.
Proc. Natl. Acad. Sci. U. S. A. 87 (1990) 682-5.
Rattus norvegicus [GN:rno] |
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Reference
Authors
Title
Journal
Organism
|
2 [PMID:1700698]
Knowles RG, Merrett M, Salter M, Moncada S.
Differential induction of brain, lung and liver nitric oxide
synthase by endotoxin in the rat.
Biochem. J. 270 (1990) 833-6.
Rattus norvegicus [GN:rno] |
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Reference
Authors
Title
Journal
Organism
|
3 [PMID:2567594]
Moncada S, Palmer RM, Higgs EA.
Biosynthesis of nitric oxide from L-arginine. A pathway for the
regulation of cell function and communication.
Biochem. Pharmacol. 38 (1989) 1709-15.
Homo sapiens [GN:hsa] |
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| Other DBs |
ExplorEnz - The Enzyme Database: 1.14.13.39
IUBMB Enzyme Nomenclature: 1.14.13.39
ExPASy - ENZYME nomenclature database: 1.14.13.39
BRENDA, the Enzyme Database: 1.14.13.39
CAS: 125978-95-2 |
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