KEGG ENZYME: 1.18.1.5

ENZYME: 1.18.1.5

Entry

EC 1.18.1.5                 Enzyme

Name

putidaredoxin---NAD+ reductase;
putidaredoxin reductase;
camA (gene name)

Class

Oxidoreductases;
Acting on iron-sulfur proteins as donors;
With NAD+ or NADP+ as acceptor

Sysname

putidaredoxin:NAD+ oxidoreductase

Reaction(IUBMB)

reduced putidaredoxin + NAD+ = oxidized putidaredoxin + NADH + H+ [RN:R10048]

Reaction(KEGG)

R10048

Substrate

reduced putidaredoxin [CPD:C02069];
NAD+ [CPD:C00003]

Product

oxidized putidaredoxin [CPD:C03302];
NADH [CPD:C00004];
H+ [CPD:C00080]

Comment

Requires FAD. The enzyme from Pseudomonas putida reduces putidaredoxin. It contains a [2Fe-2S] cluster. Involved in the camphor monooxygenase system (see EC 1.14.15.1, camphor 5-monooxygenase).

History

EC 1.18.1.5 created 2012

Orthology

K21570

putidaredoxin---NAD+ reductase

Genes

PSET:

THL1_4172

MEDK:

QEV83_05950

Reference

1 [PMID:6401738]

Authors

Roome PW Jr, Philley JC, Peterson JA

Title

Purification and properties of putidaredoxin reductase.

Journal

J Biol Chem 258:2593-8 (1983)

Reference

2 [PMID:2613690]

Authors

Koga H, Yamaguchi E, Matsunaga K, Aramaki H, Horiuchi T

Title

Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida.

Journal

J Biochem 106:831-6 (1989)
DOI:10.1093/oxfordjournals.jbchem.a122939

Reference

3 [PMID:2180940]

Authors

Peterson JA, Lorence MC, Amarneh B

Title

Putidaredoxin reductase and putidaredoxin. Cloning, sequence determination, and heterologous expression of the proteins.

Journal

J Biol Chem 265:6066-73 (1990)

Sequence

[ag:AAA25758]

Reference

4 [PMID:12011076]

Authors

Sevrioukova IF, Poulos TL

Title

Putidaredoxin reductase, a new function for an old protein.

Journal

J Biol Chem 277:25831-9 (2002)
DOI:10.1074/jbc.M201110200

Sequence

[ag:AAA25758]

Reference

5 [PMID:14529624]

Authors

Sevrioukova IF, Garcia C, Li H, Bhaskar B, Poulos TL

Title

Crystal structure of putidaredoxin, the [2Fe-2S] component of the P450cam monooxygenase system from Pseudomonas putida.

Journal

J Mol Biol 333:377-92 (2003)
DOI:10.1016/j.jmb.2003.08.028

Reference

6 [PMID:15095867]

Authors

Sevrioukova IF, Li H, Poulos TL

Title

Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase.

Journal

J Mol Biol 336:889-902 (2004)
DOI:10.1016/j.jmb.2003.12.067

Sequence

[ag:AAA25758]

Reference

7 [PMID:15103126]

Authors

Smith N, Mayhew M, Holden MJ, Kelly H, Robinson H, Heroux A, Vilker VL, Gallagher DT

Title

Structure of C73G putidaredoxin from Pseudomonas putida.

Journal

Acta Crystallogr D Biol Crystallogr 60:816-22 (2004)
DOI:10.1107/S0907444904003348

Other DBs

ExplorEnz - The Enzyme Database:

1.18.1.5

IUBMB Enzyme Nomenclature:

1.18.1.5

ExPASy - ENZYME nomenclature database:

1.18.1.5

BRENDA, the Enzyme Database:

1.18.1.5

All links

Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (1)   
   KEGG REACTION (1)   
Gene (5)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (4)   
Protein sequence (164)   
   UniProt (163)   
   SWISS-PROT (1)   
DNA sequence (108)   
   GenBank (55)   
   EMBL (53)   
Protein domain (5)   
   InterPro (5)   
All databases (288)   

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