KEGG   ENZYME: 1.5.1.20Help
Entry
EC 1.5.1.20                 Enzyme                                 
Name methylenetetrahydrofolate reductase [NAD(P)H];
methylenetetrahydrofolate (reduced nicotinamide adenine dinucleotide
phosphate) reductase;
5,10-methylenetetrahydrofolate reductase (NADPH);
5,10-methylenetetrahydrofolic acid reductase;
5,10-CH2-H4folate reductase;
methylenetetrahydrofolate reductase (NADPH2);
5-methyltetrahydrofolate:NAD+ oxidoreductase;
5-methyltetrahydrofolate:NAD+ oxidoreductase;
methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide)
reductase;
5,10-methylenetetrahydrofolate reductase;
methylenetetrahydrofolate reductase;
N5,10-methylenetetrahydrofolate reductase;
5,10-methylenetetrahydropteroylglutamate reductase;
N5,N10-methylenetetrahydrofolate reductase;
methylenetetrahydrofolic acid reductase;
5-methyltetrahydrofolate:(acceptor) oxidoreductase (incorrect);
5,10-methylenetetrahydrofolate reductase (FADH2);
MetF;
methylenetetrahydrofolate reductase (NADPH);
5-methyltetrahydrofolate:NADP+ oxidoreductase
Class Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname 5-methyltetrahydrofolate:NAD(P)+ oxidoreductase
Reaction(IUBMB) 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate
+ NAD(P)H + H+ [RN:R01224 R07168]
Reaction(KEGG) R01224 R07168
Show
Substrate 5-methyltetrahydrofolate [CPD:C00440];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product 5,10-methylenetetrahydrofolate [CPD:C00143];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Cofactor FAD [CPD:C00016]
Comment A flavoprotein (FAD). Menadione can also serve as an electron
acceptor.
Pathway PATH: ec00670  One carbon pool by folate
PATH: ec00680  Methane metabolism
PATH: ec01100  Metabolic pathways
Orthology KO: K00297  methylenetetrahydrofolate reductase (NADPH)
Genes HSA: 4524(MTHFR)
PTR: 469800(MTHFR)
MCC: 714840
MMU: 17769(Mthfr)
RNO: 362657(Mthfr)
CFA: 478230(MTHFR)
BTA: 497032(MTHFR)
ECB: 100050979
MDO: 100018128
OAA: 100086119
GGA: 419489(MTHFR)
TGU: 100220660
XLA: 379784(mthfr)
XTR: 100125082
DRE: 567547(DKEYP-100A1.4)
SPU: 589576
DME: Dmel_CG7560
DPO: Dpse_GA20440
CEL: C06A8.1
NVE: NEMVE_v1g234844
ATH: AT2G44160(MTHFR2) AT3G59970(MTHFR1)
OSA: 4334554(Os03g0815200)
CME: CMT610C
SCE: YGL125W(MET13)
AGO: AGOS_AAR170W AGOS_AFR457W
KLA: KLLA0D12188g KLLA0F09647g
DHA: DEHA0F09867g DEHA0G01551g
PIC: PICST_71674 PICST_83803
CGR: CAGL0F07029g CAGL0J09504g
YLI: YALI0B00572g YALI0B14465g
SPO: SPAC343.10(met11) SPAC56F8.10(met5)
NCR: NCU07690
MGR: MGG_01728
ANI: AN5883.2 AN8215.2
AFM: AFUA_2G11300 AFUA_5G03480
AOR: AO090026000520 AO090102000557
ANG: An02g03270 An09g05860
CNE: CNA07390 CNL04820
UMA: UM04612.1
DDI: DDB_0230137
LMA: LmjF36.6390
ECO: b3941(metF)
ECJ: JW3913(metF)
ECD: ECDH10B_4130(metF)
EBW: BWG_3610(metF)
ECE: Z5496(metF)
ECS: ECs4870(metF)
ECF: ECH74115_5402(metF)
ETW: ECSP_5011(metF)
ECG: E2348C_4250(metF)
ECC: c4899(metF)
ECI: UTI89_C4531(metF)
ECP: ECP_4155(metF)
ECV: APECO1_2525(metF)
ECW: EcE24377A_4481(metF)
ECX: EcHS_A4175(metF)
ECM: EcSMS35_4389(metF)
ECY: ECSE_4235(metF)
ECL: EcolC_4074(metF)
ECK: EC55989_4424(metF)
ECQ: ECED1_4647(metF)
ECR: ECIAI1_4150(metF)
ECT: ECIAI39_3047(metF)
ECZ: ECS88_4397(metF)
EUM: ECUMN_4473(metF)
ELF: LF82_1321(metF)
EBL: B21_03776(metF)
EBD: ECBD_4082
EBR: ECB_03827(metF)
EFE: EFER_3821(metF)
STY: STY3761(metF)
STT: t3511(metF)
SPT: SPA3951(metF)
SEK: SSPA3677(metF)
SPQ: SPAB_05087(metF)
SEI: SPC_4215(metF)
SEC: SC3997(metF)
SEH: SeHA_C4440(metF)
SEE: SNSL254_A4437(metF)
SEW: SeSA_A4323(metF)
SEA: SeAg_B4353(metF)
SED: SeD_A4511(metF)
SEG: SG3311(metF)
SET: SEN3899(metF)
SES: SARI_03551(metF)
STM: STM4105(metF)
YPE: YPO0117(metF) YPO0287
YPK: y0304(metF) y0548
YPM: YP_0119(metF) YP_0445(metF2)
YPA: YPA_0266(metF) YPA_3994
YPN: YPN_3379 YPN_3737(metF)
YPG: YpAngola_A3805(metF)
YPP: YPDSF_3682 YPDSF_3790(metF)
YPS: YPTB0107(metF) YPTB0345
YPI: YpsIP31758_0123(metF)
YPY: YPK_4093(metF)
YPB: YPTS_0110(metF)
YEN: YE0114(metF)
SFL: SF4019(metF)
SFX: S3728(metF)
SFV: SFV_4011(metF)
SSN: SSON_4115(metF)
SBO: SBO_3961(metF)
SBC: SbBS512_E4428(metF)
SDY: SDY_3776(metF)
ECA: ECA4244(metF)
PCT: PC1_0185
PWA: Pecwa_0191
ETA: ETA_01290(metF)
PLU: plu4754(metF)
PAY: PAU_04242(metF)
BUC: BU046(metF)
BAS: BUsg043(metF)
BAB: bbp047(metF)
BCC: BCc_030(metF)
BAP: BUAP5A_045(metF)
BAU: BUAPTUC7_046(metF)
SGL: SG2162(metF)
ENT: Ent638_4033(metF)
ESA: ESA_03820(metF)
CTU: Ctu_01820(metF)
KPN: KPN_04239(metF)
KPE: KPK_5441(metF)
KPU: KP1_0102(metF)
CKO: CKO_03051(metF)
SPE: Spro_4784(metF)
PMR: PMI2932(metF)
EIC: NT01EI_3851
BFL: Bfl597(metF)
BPN: BPEN_619(metF)
DDA: Dd703_3789
DDC: Dd586_3931
DZE: Dd1591_0125
HIN: HI1444(metF)
HIT: NTHI1686(metF)
HIP: CGSHiEE_04820(metF)
HIQ: CGSHiGG_01105(metF)
HSO: HS_1130(metF)
PMU: PM0235(metF)
MSU: MS0787(metF)
APL: APL_0854(metF)
APA: APP7_0912(metF)
AAP: NT05HA_0300
AAT: D11S_1097
XFA: XF1121
XFT: PD0413(metF)
XFM: Xfasm12_0466
XFN: XfasM23_0406
XCC: XCC0314(metF) XCC0739(metF)
XCB: XC_0326 XC_3496
XCA: xccb100_0337(metF2)
XCV: XCV0340 XCV0842(metF)
XAC: XAC0331(metF) XAC0791(metF)
XOO: XOO3810(metF) XOO4338(metF)
XOM: XOO_3591 XOO_4089
SML: Smlt0767(metF) Smlt2582
SMT: Smal_0614
VCH: VC2685(metF)
VCO: VC0395_A2258(metF)
VCM: VCM66_2605(metF)
VCJ: VCD_001682
VVU: VV1_1366(metF)
VVY: VV3006(metF)
VPA: VP2763(metF)
VHA: VIBHAR_00047(metF)
VSP: VS_2892(metF)
VEX: VEA_002308
VFI: VF_2309(metF)
VFM: VFMJ11_2421(metF)
VSA: VSAL_I2762(metF)
PPR: PBPRA0263(metF)
PAE: PA0430(metF)
PAU: PA14_05590(metF)
PAP: PSPA7_0530(metF)
PAG: PLES_04281(metF)
PPU: PP_4977(metF)
PPF: Pput_4850
PPG: PputGB1_5026
PPW: PputW619_0488
PST: PSPTO_5069(metF)
PSB: Psyr_0459
PSP: PSPPH_0450(metF)
PFL: PFL_5799(metF)
PFO: Pfl01_5281
PFS: PFLU5721(metF)
PEN: PSEEN5038(metF)
PMY: Pmen_0448
PSA: PST_3936(metF)
CJA: CJA_0205(metF)
AVN: Avin_03550(metF)
PAR: Psyc_0967(metF)
PCR: Pcryo_1449
PRW: PsycPRwf_0914
ACI: ACIAD2283(metF)
ACB: A1S_2335
ABM: ABSDF1186(metF) ABSDF1964
ABY: ABAYE1141(metF) ABAYE1792
ABC: ACICU_02537
ABN: AB57_2768(metF)
ABB: ABBFA_001106(metF)
SON: SO_4054(metF)
SDN: Sden_0642(metF)
SFR: Sfri_3547(metF)
SAZ: Sama_3169(metF)
SBL: Sbal_3774(metF)
SBM: Shew185_0538(metF)
SBN: Sbal195_0562(metF)
SBP: Sbal223_0566(metF)
SLO: Shew_0522(metF)
SPC: Sputcn32_0618(metF)
SSE: Ssed_4039(metF)
SPL: Spea_0562(metF)
SHE: Shewmr4_3437(metF)
SHM: Shewmr7_0514(metF)
SHN: Shewana3_3612(metF)
SHW: Sputw3181_3552(metF)
SHL: Shal_0624(metF)
SWD: Swoo_0624(metF)
PAT: Patl_3955
SDE: Sde_0468
MAQ: Maqu_3044
TTU: TERTU_3978(metF)
CBU: CBU_2047(metF)
CBS: COXBURSA331_A0036(metF)
CBD: CBUD_2143(metF)
CBG: CbuG_2052(metF)
CBC: CbuK_2094(metF)
MCA: MCA0137(metF)
TCX: Tcr_1822
NOC: Noc_2680
AEH: Mlg_2264
HHA: Hhal_1674
TGR: Tgr7_2896
HNA: Hneap_0415
HCH: HCH_01531(metF)
CSA: Csal_0396
ABO: ABO_2619(metF)
MMW: Mmwyl1_0703
AHA: AHA_3949(metF)
TAU: Tola_0061
DNO: DNO_0863(metF) DNO_1022
AFE: Lferr_0692
AFR: AFE_0535(metF)
BCI: BCI_0162(metF)
CRP: CRP_015
RMA: Rmag_0851
VOK: COSY_0776(metF)
KKO: Kkor_1610
NMA: NMA1139(metF)
NME: NMB0943(metF)
NMC: NMC0921(metF)
NMI: NMO_0840(metF)
NGO: NGO0929(metF)
CVI: CV_0966(metF)
LHK: LHK_00541(metF)
RSO: RSc0091(metF)
RPI: Rpic_3658
RPF: Rpic12D_3335
REU: Reut_A0215
REH: H16_A0246
RME: Rmet_0172 Rmet_2988
CTI: RALTA_A0190(metF)
BMA: BMA2840(metF)
BMV: BMASAVP1_A3415(metF)
BML: BMA10229_A1703(metF)
BMN: BMA10247_3136(metF)
BPS: BPSL3288(metF)
BPM: BURPS1710b_0055(metF)
BPL: BURPS1106A_3917(metF)
BPD: BURPS668_3836(metF)
BPR: GBP346_A4022(metF)
BTE: BTH_I3163(metF)
BVI: Bcep1808_0234
BUR: Bcep18194_A3377
BCN: Bcen_2833
BCH: Bcen2424_0274
BCM: Bcenmc03_0256
BCJ: BCAL0147(metF)
BAM: Bamb_0188
BAC: BamMC406_0201
BMU: Bmul_0182
BMJ: BMULJ_03082(metF)
BXE: Bxe_A0180
BPH: Bphy_2916
BPY: Bphyt_3756
BGL: bglu_1g02010
PNU: Pnuc_2001
PNE: Pnec_1717
BPE: BP3066
BPA: BPP0197
BBR: BB0200
BPT: Bpet4761(metF)
BAV: BAV0163(metF)
RFR: Rfer_0654
POL: Bpro_4130
PNA: Pnap_0491
AAV: Aave_4058
AJS: Ajs_0620
VEI: Veis_0654
DAC: Daci_5917
DIA: Dtpsy_0599
VAP: Vapar_4681
MPT: Mpe_A3288
HAR: HEAR2948(metF)
MMS: mma_3194(metF)
LCH: Lcho_0117
NEU: NE0661(metF)
NET: Neut_1891
NMU: Nmul_A2539
EBA: ebA1875(metF)
AZO: azo0584(metF)
DAR: Daro_0183
TBD: Tbd_2517
MFA: Mfla_0191
MMB: Mmol_2189
MEI: Msip34_0180
APP: CAP2UW1_0395
CJE: Cj1202(metF)
CJR: CJE1336(metF)
CJJ: CJJ81176_1217(metF)
CJU: C8J_1146(metF)
GLO: Glov_2130
GBM: Gbem_3302
GEM: GM21_0946
PCA: Pcar_1732(metF)
PPD: Ppro_1089
DVU: DVU0997(metF)
DVL: Dvul_1992
DVM: DvMF_3034
DDE: Dde_2525
DDS: Ddes_2008
DMA: DMR_16040(metF)
DSA: Desal_2300
DBA: Dbac_1758
DRT: Dret_1105
DPS: DP1612
DOL: Dole_0458 Dole_1151 Dole_2330
DAL: Dalk_1779 Dalk_2779
DAT: HRM2_16730(metF1) HRM2_30790(metF2) HRM2_30860(metF3)
ADE: Adeh_2676
ACP: A2cp1_2862
ANK: AnaeK_2769
MXA: MXAN_3039(metF)
SCL: sce8996
SAT: SYN_02872
SFU: Sfum_3130
PUB: SAR11_1264(metF)
MLO: mll1587
MES: Meso_2028
PLA: Plav_2401
SME: SMa2143(metF) SMc01843(metF)
ATU: Atu1418(metF) Atu2123(metF)
ATC: AGR_C_2619 AGR_C_3850
RET: RHE_CH02871(metF)
REC: RHECIAT_CH0003023(metF)
RLE: RL3331(metF) pRL90256
RLT: Rleg2_2618
RLG: Rleg_2878
BME: BMEI0559
BMF: BAB1_1468(metF)
BMB: BruAb1_1445(metF)
BMC: BAbS19_I13730
BMS: BR1450(metF)
BOV: BOV_1406(metF)
BMR: BMI_I1462(metF)
OAN: Oant_1723
BJA: bll1419(metF)
BRA: BRADO0995(metF)
BBT: BBta_7060(metF)
RPA: RPA3701(metF)
RPB: RPB_1765
RPC: RPC_3734
RPD: RPD_3540
RPE: RPE_3772
RPT: Rpal_4223
NWI: Nwi_0284
NHA: Nham_0364
OCA: OCAR_7438(metF)
XAU: Xaut_2831
MEX: Mext_1600
MEA: Mex_1p1493(metF)
MDI: METDI2266(metF)
MRD: Mrad2831_2124
MET: M446_4365
MPO: Mpop_1639
MCH: Mchl_1881 Mchl_5726
MNO: Mnod_4919
BID: Bind_1122
MSL: Msil_3544
CCR: CC_2140
CCS: CCNA_02224
CAK: Caul_3409
SIL: SPO1862 SPO3016(metF)
SIT: TM1040_1185 TM1040_1700
RSP: RSP_2172(metF) RSP_2769
RSH: Rsph17029_0844 Rsph17029_1411
RSQ: Rsph17025_1029 Rsph17025_2321
JAN: Jann_1026 Jann_1978
RDE: RD1_1762(metF) RD1_3056(metF)
PDE: Pden_2319
DSH: Dshi_1675 Dshi_2080(metF)
MMR: Mmar10_2438
HNE: HNE_2103(metF)
HBA: Hbal_0372
ZMO: ZMO1747
ZMN: Za10_1478
NAR: Saro_2860 Saro_3208
SAL: Sala_0035
SWI: Swit_2401
ELI: ELI_10095
GOX: GOX0791 GOX2207
GBE: GbCGDNIH1_0206
ACR: Acry_0219
GDI: GDI_1306 GDI_2575(metF)
GDJ: Gdia_0809
RRU: Rru_A1530
RCE: RC1_2050(metF)
MGM: Mmc1_1506
BCL: ABC1868
LLA: L0099(metF)
LLK: LLKF_1319(metF)
LLC: LACR_1367
LLM: llmg_1226(metF)
SPN: SP_0586
SPD: SPD_0511(metF)
SPR: spr0515(metF)
SPW: SPCG_0552(metF)
SPX: SPG_0535(metF)
SNE: SPN23F_05300(metF)
SPV: SPH_0685(metF)
SNM: SP70585_0651(metF)
SJJ: SPJ_0541(metF)
SPP: SPP_0603(metF)
SNT: SPT_0616(metF)
STC: str0786(metF)
STL: stu0786(metF)
STE: STER_0832
SSA: SSA_0417(metF)
SSU: SSU05_1775
SSV: SSU98_1785
SSB: SSUBM407_1654(metF)
SSI: SSU1580(metF)
SSS: SSUSC84_1606(metF)
SGO: SGO_0311(metF)
LBU: LBUL_1231
LCA: LSEI_0624
LCB: LCABL_06820(metF)
LRH: LGG_00596(metF)
LRL: LC705_00573(metF)
LME: LEUM_1795
CTH: Cthe_0099
CBO: CBO1621(metF)
CBA: CLB_1639(metF)
CBH: CLC_1648(metF)
CBY: CLM_1863(metF)
CBL: CLK_1085(metF)
CBK: CLL_A2856(metF)
CBB: CLD_2935(metF)
CBI: CLJ_B1732(metF)
CBT: CLH_2584(metF)
CBF: CLI_1699(metF)
CPY: Cphy_3110
AMT: Amet_3375
AOE: Clos_0078
SWO: Swol_0934
DRM: Dred_2192
DAE: Dtox_0534 Dtox_1285
DAU: Daud_0880
CHY: CHY_1233
MTA: Moth_1191
MAB: MAB_1993
CGL: NCgl2091(cgl2171)
CGB: cg2383(metF)
CGT: cgR_2053
CEF: CE2066
CDI: DIP1611
CJK: jk0737(metF)
CUR: cur_1218
NFA: nfa17400(metF)
RHA: RHA1_ro01105
RER: RER_35690(metF)
ROP: ROP_08330(metF)
GBR: Gbro_3032
SCO: SCO2103(metF)
SMA: SAV_6100(metF)
ART: Arth_0063 Arth_2367
ACH: Achl_2125
RSA: RSal33209_3453
KRH: KRH_20900(metF)
BCV: Bcav_0238
JDE: Jden_0190
XCE: Xcel_0051
NCA: Noca_3083
TFU: Tfu_1050
TCU: Tcur_3026
SRO: Sros_2788
FRA: Francci3_1389
FRE: Franean1_5126
FAL: FRAAL2163(metF)
ACE: Acel_0991
NML: Namu_3198
KRA: Krad_0936 Krad_1170
SEN: SACE_1715(metF) SACE_3895(metF) SACE_5618(metF)
AMI: Amir_1401
STP: Strop_3250
SAQ: Sare_3477
CAI: Caci_1572
BLO: BL0797(metF)
BAD: BAD_0757(metF)
RXY: Rxyl_0765
AFO: Afer_0324
LIL: LB002(metF)
LIC: LIC20002(metF)
LBJ: LBJ_4004(metF)
LBL: LBL_4004(metF)
LBI: LEPBI_I3275(metF)
BTH: BT_3821
BFR: BF4092
BFS: BF3908
SRU: SRU_2094(metF)
RMR: Rmar_0234
CHU: CHU_1435(metF)
DFE: Dfer_2651
CPI: Cpin_0867
GFO: GFO_0334(metF)
FJO: Fjoh_1511
FBA: FIC_02050
COC: Coch_0413
BBL: BLBBGE_445(metF)
FSU: Fisuc_0576
LBA: Lebu_0488
STR: Sterm_2170
OTE: Oter_4187
AMU: Amuc_1055
RBA: RB7546(metF)
TAI: Taci_0142
SYG: sync_2618
SYR: SynRCC307_0227(metF)
SYX: SynWH7803_2283(metF)
SYP: SYNPCC7002_A0038(metF)
MAR: MAE_39290
CYT: cce_3533
CYP: PCC8801_2050
CYC: PCC7424_0350
CYN: Cyan7425_1687
CYH: Cyan8802_2074
NPU: Npun_R5069
AVA: Ava_2992
PMA: Pro0176(metF)
PMT: PMT2013
PMN: PMN2A_1519
PMB: A9601_01701(metF)
PMC: P9515_01811(metF)
PMF: P9303_26821(metF)
PMG: P9301_01721(metF)
PMJ: P9211_01691
PME: NATL1_02251(metF)
TER: Tery_4692
AMR: AM1_0791
CTE: CT1368(metF)
CPC: Cpar_0790
CCH: Cag_1108
CPH: Cpha266_1723
CPB: Cphamn1_0977
CLI: Clim_1552
PVI: Cvib_0706
PLT: Plut_1347
PPH: Ppha_1647
PAA: Paes_1498
RRS: RoseRS_2032
RCA: Rcas_3006
CAU: Caur_1457
CAG: Cagg_1947
CHL: Chy400_1580
DGE: Dgeo_1337
TTH: TTC1656
TTJ: TTHA0327
AAE: aq_1429(metF)
HYA: HY04AAS1_1567
SUL: SYO3AOP1_0477
SAF: SULAZ_0776(metF)
PMX: PERMA_0068(metF)
TME: Tmel_0731
PMO: Pmob_0318
MMA: MM_0438
HMA: pNG7363(metF)
HWA: HQ1756A(metF)
Taxonomy
Structures PDB: 2FMN  2FMO  3FST  3FSU  
Reference
  Authors
  Title

  Journal
  Organism
 1  [PMID:6975779]
Daubner SC, Matthews RG.
Purification and properties of methylenetetrahydrofolate reductase
from pig liver.
J. Biol. Chem. 257 (1982) 140-5.
Sus scofa [GN:ssc]
Reference
  Authors
  Title

  Journal
  Organism
 2  [PMID:4399897]
Kutzbach C, Stokstad EL.
Mammalian methylenetetrahydrofolate reductase. Partial purification,
properties, and inhibition by S-adenosylmethionine.
Biochim. Biophys. Acta. 250 (1971) 459-77.
Rattus norvegicus [GN:rno], Sus scofa [GN:ssc]
Reference
  Authors
  Title

  Journal
  Organism
 3  [PMID:9922232]
Sheppard CA, Trimmer EE, Matthews RG.
Purification and properties of NADH-dependent 5,
10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli.
J. Bacteriol. 181 (1999) 718-25.
Escherichia coli [GN:eco]
Reference
  Authors
  Title


  Journal
  Organism
 4  [PMID:10201405]
Guenther BD, Sheppard CA, Tran P, Rozen R, Matthews RG, Ludwig ML.
The structure and properties of methylenetetrahydrofolate reductase
from Escherichia coli suggest how folate ameliorates human
hyperhomocysteinemia.
Nat. Struct. Biol. 6 (1999) 359-65.
Escherichia coli [GN:eco]
Other DBs ExplorEnz - The Enzyme Database: 1.5.1.20
IUBMB Enzyme Nomenclature: 1.5.1.20
ExPASy - ENZYME nomenclature database: 1.5.1.20
BRENDA, the Enzyme Database: 1.5.1.20
CAS: 71822-25-8
DBGET integrated database retrieval system