KEGG   ENZYME: 2.4.2.36Help
Entry
EC 2.4.2.36                 Enzyme                                 

Name
NAD+---diphthamide ADP-ribosyltransferase;
ADP-ribosyltransferase;
mono(ADPribosyl)transferase;
NAD---diphthamide ADP-ribosyltransferase;
NAD+:peptide-diphthamide N-(ADP-D-ribosyl)transferase
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
NAD+:diphthamide-[translation elongation factor 2] N-(ADP-D-ribosyl)transferase
Reaction(IUBMB)
NAD+ + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2] [RN:R04074]
Reaction(KEGG)
Substrate
NAD+ [CPD:C00003];
diphthamide-[translation elongation factor 2]
Product
nicotinamide [CPD:C00153];
N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Comment
Diphtheria toxin and some other bacterial toxins catalyse this reaction, which inactivates translation elongation factor 2 (EF2). The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. cf. EC 2.4.2.31 NAD(P)+---protein-arginine ADP-ribosyltransferase. The relevant histidine of EF2 is His715 in mammals, His699 in yeast and His600 in Pyrococcus horikoshii.
History
EC 2.4.2.36 created 1990, modified 2013
Orthology
K00776  
NAD-diphthamide ADP-ribosyltransferase
K10928  
cholera enterotoxin subunit A
Genes
ECW: 
ELH: 
EUN: 
VCH: 
VCE: 
VCJ: 
VCO: 
VCR: 
VCI: 
CDI: 
DIP0222(tox)
CDZ: 
CDS: 
CDD: 
CDW: 
COP: 
CUE: 
 » show all
Taxonomy
Reference
1  [PMID:6326138]
  Authors
Lee H, Iglewski WJ.
  Title
Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 81 (1984) 2703-7.
Reference
2  [PMID:3927821]
  Authors
Ueda K, Hayaishi O.
  Title
ADP-ribosylation.
  Journal
Annu. Rev. Biochem. 54 (1985) 73-100.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
52933-21-8

DBGET integrated database retrieval system