Kudo F, Eguchi T

Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.

Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5

Kudo F, Eguchi T

Biosynthetic genes for aminoglycoside antibiotics.

J Antibiot (Tokyo) 62:471-81 (2009)
DOI:10.1038/ja.2009.76

Nango E, Kumasaka T, Hirayama T, Tanaka N, Eguchi T

Structure of 2-deoxy-scyllo-inosose synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics, in complex with a mechanism-based inhibitor and NAD+.

Proteins 70:517-27 (2008)
DOI:10.1002/prot.21526

Yokoyama K, Kudo F, Kuwahara M, Inomata K, Tamegai H, Eguchi T, Kakinuma K

Stereochemical recognition of doubly functional aminotransferase in 2-deoxystreptamine biosynthesis.

J Am Chem Soc 127:5869-74 (2005)
DOI:10.1021/ja0445948

Tamegai H, Nango E, Kuwahara M, Yamamoto H, Ota Y, Kuriki H, Eguchi T, Kakinuma K

Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.

J Antibiot (Tokyo) 55:707-14 (2002)
DOI:10.7164/antibiotics.55.707

Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K

Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.

J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104

Yokoyama K, Ohmori D, Kudo F, Eguchi T

Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.

Biochemistry 47:8950-60 (2008)
DOI:10.1021/bi800509x

Yokoyama K, Numakura M, Kudo F, Ohmori D, Eguchi T

Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin.

J Am Chem Soc 129:15147-55 (2007)
DOI:10.1021/ja072481t

Yokoyama K, Yamamoto Y, Kudo F, Eguchi T

Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function deacetylase in neomycin biosynthesis.

Chembiochem 9:865-9 (2008)
DOI:10.1002/cbic.200700717

Fan Q, Huang F, Leadlay PF, Spencer JB

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: genetic and biochemical evidence for the roles of two glycosyltransferases and a deacetylase.

Org Biomol Chem 6:3306-14 (2008)
DOI:10.1039/b808734b

Truman AW, Huang F, Llewellyn NM, Spencer JB

Characterization of the enzyme BtrD from Bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin.

Angew Chem Int Ed Engl 46:1462-4 (2007)
DOI:10.1002/anie.200604194

Kudo F, Fujii T, Kinoshita S, Eguchi T

Unique O-ribosylation in the biosynthesis of butirosin.

Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040

Yukita T, Nishida H, Eguchi T, Kakinuma K

Biosynthesis of (2R)-4-amino-2-hydroxybutyric acid, unique and biologically significant substituent in butirosins.

J Antibiot (Tokyo) 56:497-500 (2003)
DOI:10.7164/antibiotics.56.497

Li Y, Llewellyn NM, Giri R, Huang F, Spencer JB

Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.

Chem Biol 12:665-75 (2005)
DOI:10.1016/j.chembiol.2005.04.010

Llewellyn NM, Li Y, Spencer JB

Biosynthesis of butirosin: transfer and deprotection of the unique amino acid side chain.

Chem Biol 14:379-86 (2007)
DOI:10.1016/j.chembiol.2007.02.005

Llewellyn NM, Spencer JB

Chemoenzymatic acylation of aminoglycoside antibiotics.

Chem Commun (Camb) 3786-8 (2008)
DOI:10.1039/b802248h

Nepal KK, Oh TJ, Sohng JK

Heterologous production of paromamine in Streptomyces lividans TK24 using kanamycin biosynthetic genes from Streptomyces kanamyceticus ATCC12853.

Mol Cells 27:601-8 (2009)
DOI:10.1007/s10059-009-0080-5

Park JW, Park SR, Nepal KK, Han AR, Ban YH, Yoo YJ, Kim EJ, Kim EM, Kim D, Sohng JK, Yoon YJ

Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation.

Nat Chem Biol 7:843-52 (2011)
DOI:10.1038/nchembio.671

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Aminoglycoside antibiotics: From chemical biology to drug discovery

Wiley-Interscience (2007)

Park SR, Park JW, Ban YH, Sohng JK, Yoon YJ

2-Deoxystreptamine-containing aminoglycoside antibiotics: recent advances in the characterization and manipulation of their biosynthetic pathways.

Nat Prod Rep 30:11-20 (2013)
DOI:10.1039/c2np20092a

Llewellyn NM, Spencer JB

Biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics.

Nat Prod Rep 23:864-74 (2006)
DOI:10.1039/b604709m

Kudo F, Eguchi T

Aminoglycoside Antibiotics: New Insights into the Biosynthetic Machinery of Old Drugs.

Chem Rec 16:4-18 (2016)
DOI:10.1002/tcr.201500210

Takeishi R, Kudo F, Numakura M, Eguchi T

Epimerization at C-3'' in butirosin biosynthesis by an NAD(+) -dependent dehydrogenase BtrE and an NADPH-dependent reductase BtrF.

Chembiochem 16:487-95 (2015)
DOI:10.1002/cbic.201402612

Lv M, Ji X, Zhao J, Li Y, Zhang C, Su L, Ding W, Deng Z, Yu Y, Zhang Q

Characterization of a C3 Deoxygenation Pathway Reveals a Key Branch Point in Aminoglycoside Biosynthesis.

J Am Chem Soc 138:6427-35 (2016)
DOI:10.1021/jacs.6b02221

Clausnitzer D, Piepersberg W, Wehmeier UF

The oxidoreductases LivQ and NeoQ are responsible for the different 6'-modifications in the aminoglycosides lividomycin and neomycin.

J Appl Microbiol 111:642-51 (2011)
DOI:10.1111/j.1365-2672.2011.05082.x

Park JW, Hong JS, Parajuli N, Jung WS, Park SR, Lim SK, Sohng JK, Yoon YJ

Genetic dissection of the biosynthetic route to gentamicin A2 by heterologous expression of its minimal gene set.

Proc Natl Acad Sci U S A 105:8399-404 (2008)
DOI:10.1073/pnas.0803164105

Huang C, Huang F, Moison E, Guo J, Jian X, Duan X, Deng Z, Leadlay PF, Sun Y

Delineating the biosynthesis of gentamicin x2, the common precursor of the gentamicin C antibiotic complex.

Chem Biol 22:251-61 (2015)
DOI:10.1016/j.chembiol.2014.12.012

Guo J, Huang F, Huang C, Duan X, Jian X, Leeper F, Deng Z, Leadlay PF, Sun Y

Specificity and promiscuity at the branch point in gentamicin biosynthesis.

Chem Biol 21:608-18 (2014)
DOI:10.1016/j.chembiol.2014.03.005

Gu Y, Ni X, Ren J, Gao H, Wang D, Xia H

Biosynthesis of Epimers C2 and C2a in the Gentamicin C Complex.

Chembiochem 16:1933-42 (2015)
DOI:10.1002/cbic.201500258

Kim HJ, McCarty RM, Ogasawara Y, Liu YN, Mansoorabadi SO, LeVieux J, Liu HW

GenK-catalyzed C-6' methylation in the biosynthesis of gentamicin: isolation and characterization of a cobalamin-dependent radical SAM enzyme.

J Am Chem Soc 135:8093-6 (2013)
DOI:10.1021/ja312641f