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Database: PDB
Entry: 2H8N
LinkDB: 2H8N
Original site: 2H8N 
HEADER    TRANSCRIPTION                           07-JUN-06   2H8N              
TITLE     STRUCTURE OF A GLUTAMINE-RICH DOMAIN FROM HISTONE                     
TITLE    2 DEACETYLASE 4                                                        
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HISTONE DEACETYLASE 4;                                     
COMPND   3 CHAIN: A, B, C, D;                                                   
COMPND   4 FRAGMENT: N-TERMINAL GLUTAMINE-RICH DOMAIN, RESIDUES 62-             
COMPND   5 129;                                                                 
COMPND   6 SYNONYM: HD4;                                                        
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: HDAC4, KIAA0288;                                               
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: ROSETTA PLYSS;                             
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28A                                    
KEYWDS    ALPHA HELIX, POLAR ZIPPER, TRANSCRIPTION                              
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    L.GUO,A.HAN,D.L.BATES,L.CHEN                                          
REVDAT   3   24-FEB-09 2H8N    1       VERSN                                    
REVDAT   2   03-APR-07 2H8N    1       JRNL                                     
REVDAT   1   27-FEB-07 2H8N    0                                                
JRNL        AUTH   L.GUO,A.HAN,D.L.BATES,J.CAO,L.CHEN                           
JRNL        TITL   CRYSTAL STRUCTURE OF A CONSERVED N-TERMINAL DOMAIN           
JRNL        TITL 2 OF HISTONE DEACETYLASE 4 REVEALS FUNCTIONAL                  
JRNL        TITL 3 INSIGHTS INTO GLUTAMINE-RICH DOMAINS.                        
JRNL        REF    PROC.NATL.ACAD.SCI.USA        V. 104  4297 2007              
JRNL        REFN                   ISSN 0027-8424                               
JRNL        PMID   17360518                                                     
JRNL        DOI    10.1073/PNAS.0608041104                                      
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.60 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CNS 1.1                                              
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-              
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,             
REMARK   3               : READ,RICE,SIMONSON,WARREN                            
REMARK   3                                                                      
REMARK   3  REFINEMENT TARGET : ENGH & HUBER                                    
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.60                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 6.00                           
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 2.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : NULL                           
REMARK   3   NUMBER OF REFLECTIONS             : 16845                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : NULL                            
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.281                           
REMARK   3   FREE R VALUE                     : 0.307                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL                            
REMARK   3   FREE R VALUE TEST SET COUNT      : 1702                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2328                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 0                                       
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 93.34                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : 42.39200                                             
REMARK   3    B22 (A**2) : -24.86500                                            
REMARK   3    B33 (A**2) : -17.52800                                            
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 7.49400                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : NULL                            
REMARK   3   BOND ANGLES            (DEGREES) : NULL                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : 2.801 ; 1.500                
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : 4.822 ; 2.000                
REMARK   3   SIDE-CHAIN BOND              (A**2) : 4.797 ; 2.000                
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : 8.211 ; 2.500                
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   KSOL        : NULL                                                 
REMARK   3   BSOL        : NULL                                                 
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM                              
REMARK   3  PARAMETER FILE  2  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3  TOPOLOGY FILE  2   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2H8N COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-JUN-06.                  
REMARK 100 THE RCSB ID CODE IS RCSB038067.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 02-JUL-04                          
REMARK 200  TEMPERATURE           (KELVIN) : 100.0                              
REMARK 200  PH                             : 7.50                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : ALS                                
REMARK 200  BEAMLINE                       : 8.2.2                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.12710                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 315                   
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : ADSC                               
REMARK 200  DATA SCALING SOFTWARE          : HKL-2000                           
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 19701                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.600                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 6.000                              
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 2.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.0                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : NULL                               
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.60                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.73                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 99.0                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : NULL                               
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: SAD                          
REMARK 200 SOFTWARE USED: CNS                                                   
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: THE REFLECTIONS IN THE STRUCTURE FACTOR FILE WERE            
REMARK 200  SCALED TO 2.3A, THE STRUCTURE WAS REFINED TO 2.6A. ALTHOUGH         
REMARK 200  THE DIFFRACTION WENT TO 2.3A, DUE TO SEVERE ANISOTROPIC             
REMARK 200  EFFECT, USEFUL DATA WAS ONLY TO 2.6A.                               
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 58.16                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.94                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 0.825M LITHIUM SULFATE, 55MM HEPES       
REMARK 280  PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K, PH         
REMARK 280  7.50                                                                
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   X+1/2,Y+1/2,Z                                           
REMARK 290       4555   -X+1/2,Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       93.69900            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       30.22550            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       93.69900            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       30.22550            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 300 REMARK: THE TETRAMER IN THE ASYMMETRIC UNIT IS THE BIOLOGICAL        
REMARK 300 ASSEMBLY, NO CRYSTALLOGRAPHIC SYMMETRY IS INVOVLED IN THE            
REMARK 300 TETRAMER FORMATION.                                                  
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC                        
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC                 
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 10050 ANGSTROM**2                         
REMARK 350 SURFACE AREA OF THE COMPLEX: 17790 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -69.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D                            
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A    42                                                      
REMARK 465     SER A    43                                                      
REMARK 465     SER A    44                                                      
REMARK 465     HIS A    45                                                      
REMARK 465     HIS A    46                                                      
REMARK 465     HIS A    47                                                      
REMARK 465     HIS A    48                                                      
REMARK 465     HIS A    49                                                      
REMARK 465     HIS A    50                                                      
REMARK 465     SER A    51                                                      
REMARK 465     SER A    52                                                      
REMARK 465     GLY A    53                                                      
REMARK 465     LEU A    54                                                      
REMARK 465     VAL A    55                                                      
REMARK 465     PRO A    56                                                      
REMARK 465     ARG A    57                                                      
REMARK 465     GLY A    58                                                      
REMARK 465     SER A    59                                                      
REMARK 465     HIS A    60                                                      
REMARK 465     MET A    61                                                      
REMARK 465     HIS A   130                                                      
REMARK 465     GLN A   131                                                      
REMARK 465     ARG A   132                                                      
REMARK 465     LYS A   133                                                      
REMARK 465     LEU A   134                                                      
REMARK 465     GLU A   135                                                      
REMARK 465     ARG A   136                                                      
REMARK 465     HIS A   137                                                      
REMARK 465     ARG A   138                                                      
REMARK 465     GLN A   139                                                      
REMARK 465     GLU A   140                                                      
REMARK 465     GLN A   141                                                      
REMARK 465     GLU A   142                                                      
REMARK 465     LEU A   143                                                      
REMARK 465     GLU A   144                                                      
REMARK 465     LYS A   145                                                      
REMARK 465     GLN A   146                                                      
REMARK 465     HIS A   147                                                      
REMARK 465     ARG A   148                                                      
REMARK 465     GLU A   149                                                      
REMARK 465     GLN A   150                                                      
REMARK 465     LYS A   151                                                      
REMARK 465     LEU A   152                                                      
REMARK 465     GLN A   153                                                      
REMARK 465     GLY B    42                                                      
REMARK 465     SER B    43                                                      
REMARK 465     SER B    44                                                      
REMARK 465     HIS B    45                                                      
REMARK 465     HIS B    46                                                      
REMARK 465     HIS B    47                                                      
REMARK 465     HIS B    48                                                      
REMARK 465     HIS B    49                                                      
REMARK 465     HIS B    50                                                      
REMARK 465     SER B    51                                                      
REMARK 465     SER B    52                                                      
REMARK 465     GLY B    53                                                      
REMARK 465     LEU B    54                                                      
REMARK 465     VAL B    55                                                      
REMARK 465     PRO B    56                                                      
REMARK 465     ARG B    57                                                      
REMARK 465     GLY B    58                                                      
REMARK 465     SER B    59                                                      
REMARK 465     HIS B    60                                                      
REMARK 465     MET B    61                                                      
REMARK 465     HIS B   130                                                      
REMARK 465     GLN B   131                                                      
REMARK 465     ARG B   132                                                      
REMARK 465     LYS B   133                                                      
REMARK 465     LEU B   134                                                      
REMARK 465     GLU B   135                                                      
REMARK 465     ARG B   136                                                      
REMARK 465     HIS B   137                                                      
REMARK 465     ARG B   138                                                      
REMARK 465     GLN B   139                                                      
REMARK 465     GLU B   140                                                      
REMARK 465     GLN B   141                                                      
REMARK 465     GLU B   142                                                      
REMARK 465     LEU B   143                                                      
REMARK 465     GLU B   144                                                      
REMARK 465     LYS B   145                                                      
REMARK 465     GLN B   146                                                      
REMARK 465     HIS B   147                                                      
REMARK 465     ARG B   148                                                      
REMARK 465     GLU B   149                                                      
REMARK 465     GLN B   150                                                      
REMARK 465     LYS B   151                                                      
REMARK 465     LEU B   152                                                      
REMARK 465     GLN B   153                                                      
REMARK 465     GLY C    42                                                      
REMARK 465     SER C    43                                                      
REMARK 465     SER C    44                                                      
REMARK 465     HIS C    45                                                      
REMARK 465     HIS C    46                                                      
REMARK 465     HIS C    47                                                      
REMARK 465     HIS C    48                                                      
REMARK 465     HIS C    49                                                      
REMARK 465     HIS C    50                                                      
REMARK 465     SER C    51                                                      
REMARK 465     SER C    52                                                      
REMARK 465     GLY C    53                                                      
REMARK 465     LEU C    54                                                      
REMARK 465     VAL C    55                                                      
REMARK 465     PRO C    56                                                      
REMARK 465     ARG C    57                                                      
REMARK 465     GLY C    58                                                      
REMARK 465     SER C    59                                                      
REMARK 465     HIS C    60                                                      
REMARK 465     MET C    61                                                      
REMARK 465     HIS C   130                                                      
REMARK 465     GLN C   131                                                      
REMARK 465     ARG C   132                                                      
REMARK 465     LYS C   133                                                      
REMARK 465     LEU C   134                                                      
REMARK 465     GLU C   135                                                      
REMARK 465     ARG C   136                                                      
REMARK 465     HIS C   137                                                      
REMARK 465     ARG C   138                                                      
REMARK 465     GLN C   139                                                      
REMARK 465     GLU C   140                                                      
REMARK 465     GLN C   141                                                      
REMARK 465     GLU C   142                                                      
REMARK 465     LEU C   143                                                      
REMARK 465     GLU C   144                                                      
REMARK 465     LYS C   145                                                      
REMARK 465     GLN C   146                                                      
REMARK 465     HIS C   147                                                      
REMARK 465     ARG C   148                                                      
REMARK 465     GLU C   149                                                      
REMARK 465     GLN C   150                                                      
REMARK 465     LYS C   151                                                      
REMARK 465     LEU C   152                                                      
REMARK 465     GLN C   153                                                      
REMARK 465     GLY D    42                                                      
REMARK 465     SER D    43                                                      
REMARK 465     SER D    44                                                      
REMARK 465     HIS D    45                                                      
REMARK 465     HIS D    46                                                      
REMARK 465     HIS D    47                                                      
REMARK 465     HIS D    48                                                      
REMARK 465     HIS D    49                                                      
REMARK 465     HIS D    50                                                      
REMARK 465     SER D    51                                                      
REMARK 465     SER D    52                                                      
REMARK 465     GLY D    53                                                      
REMARK 465     LEU D    54                                                      
REMARK 465     VAL D    55                                                      
REMARK 465     PRO D    56                                                      
REMARK 465     ARG D    57                                                      
REMARK 465     GLY D    58                                                      
REMARK 465     SER D    59                                                      
REMARK 465     HIS D    60                                                      
REMARK 465     MET D    61                                                      
REMARK 465     HIS D   130                                                      
REMARK 465     GLN D   131                                                      
REMARK 465     ARG D   132                                                      
REMARK 465     LYS D   133                                                      
REMARK 465     LEU D   134                                                      
REMARK 465     GLU D   135                                                      
REMARK 465     ARG D   136                                                      
REMARK 465     HIS D   137                                                      
REMARK 465     ARG D   138                                                      
REMARK 465     GLN D   139                                                      
REMARK 465     GLU D   140                                                      
REMARK 465     GLN D   141                                                      
REMARK 465     GLU D   142                                                      
REMARK 465     LEU D   143                                                      
REMARK 465     GLU D   144                                                      
REMARK 465     LYS D   145                                                      
REMARK 465     GLN D   146                                                      
REMARK 465     HIS D   147                                                      
REMARK 465     ARG D   148                                                      
REMARK 465     GLU D   149                                                      
REMARK 465     GLN D   150                                                      
REMARK 465     LYS D   151                                                      
REMARK 465     LEU D   152                                                      
REMARK 465     GLN D   153                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    GLU A  63      -72.58   -165.96                                   
REMARK 500    GLN A 114      -25.77   -150.07                                   
REMARK 500    HIS A 123      -36.84   -142.15                                   
REMARK 500    GLU B  63      -68.14   -160.05                                   
REMARK 500    ALA B 120      -73.65    -68.94                                   
REMARK 500    MET B 121       -5.50    -57.52                                   
REMARK 500    GLU C  63      -65.01   -171.17                                   
REMARK 500    ALA C 120      -76.41    -64.05                                   
REMARK 500    MET C 121       -5.45    -58.69                                   
REMARK 500    GLU D  63      -68.95   -172.27                                   
REMARK 500    GLN D 116      -74.71    -76.98                                   
REMARK 500    ALA D 120      -72.79    -72.35                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  2H8N A   62   153  UNP    P56524   HDAC4_HUMAN     62    153             
DBREF  2H8N B   62   153  UNP    P56524   HDAC4_HUMAN     62    153             
DBREF  2H8N C   62   153  UNP    P56524   HDAC4_HUMAN     62    153             
DBREF  2H8N D   62   153  UNP    P56524   HDAC4_HUMAN     62    153             
SEQADV 2H8N GLY A   42  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER A   43  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER A   44  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS A   45  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS A   46  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS A   47  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS A   48  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS A   49  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS A   50  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N SER A   51  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER A   52  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY A   53  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N LEU A   54  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N VAL A   55  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N PRO A   56  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N ARG A   57  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY A   58  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER A   59  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS A   60  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N MET A   61  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY B   42  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER B   43  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER B   44  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS B   45  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS B   46  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS B   47  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS B   48  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS B   49  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS B   50  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N SER B   51  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER B   52  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY B   53  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N LEU B   54  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N VAL B   55  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N PRO B   56  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N ARG B   57  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY B   58  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER B   59  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS B   60  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N MET B   61  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY C   42  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER C   43  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER C   44  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS C   45  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS C   46  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS C   47  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS C   48  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS C   49  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS C   50  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N SER C   51  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER C   52  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY C   53  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N LEU C   54  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N VAL C   55  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N PRO C   56  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N ARG C   57  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY C   58  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER C   59  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS C   60  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N MET C   61  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY D   42  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER D   43  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER D   44  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS D   45  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS D   46  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS D   47  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS D   48  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS D   49  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N HIS D   50  UNP  P56524              EXPRESSION TAG                 
SEQADV 2H8N SER D   51  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER D   52  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY D   53  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N LEU D   54  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N VAL D   55  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N PRO D   56  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N ARG D   57  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N GLY D   58  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N SER D   59  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N HIS D   60  UNP  P56524              CLONING ARTIFACT               
SEQADV 2H8N MET D   61  UNP  P56524              CLONING ARTIFACT               
SEQRES   1 A  112  GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY LEU          
SEQRES   2 A  112  VAL PRO ARG GLY SER HIS MET ALA GLU PRO ALA LEU ARG          
SEQRES   3 A  112  GLU GLN GLN LEU GLN GLN GLU LEU LEU ALA LEU LYS GLN          
SEQRES   4 A  112  LYS GLN GLN ILE GLN ARG GLN ILE LEU ILE ALA GLU PHE          
SEQRES   5 A  112  GLN ARG GLN HIS GLU GLN LEU SER ARG GLN HIS GLU ALA          
SEQRES   6 A  112  GLN LEU HIS GLU HIS ILE LYS GLN GLN GLN GLU MET LEU          
SEQRES   7 A  112  ALA MET LYS HIS GLN GLN GLU LEU LEU GLU HIS GLN ARG          
SEQRES   8 A  112  LYS LEU GLU ARG HIS ARG GLN GLU GLN GLU LEU GLU LYS          
SEQRES   9 A  112  GLN HIS ARG GLU GLN LYS LEU GLN                              
SEQRES   1 B  112  GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY LEU          
SEQRES   2 B  112  VAL PRO ARG GLY SER HIS MET ALA GLU PRO ALA LEU ARG          
SEQRES   3 B  112  GLU GLN GLN LEU GLN GLN GLU LEU LEU ALA LEU LYS GLN          
SEQRES   4 B  112  LYS GLN GLN ILE GLN ARG GLN ILE LEU ILE ALA GLU PHE          
SEQRES   5 B  112  GLN ARG GLN HIS GLU GLN LEU SER ARG GLN HIS GLU ALA          
SEQRES   6 B  112  GLN LEU HIS GLU HIS ILE LYS GLN GLN GLN GLU MET LEU          
SEQRES   7 B  112  ALA MET LYS HIS GLN GLN GLU LEU LEU GLU HIS GLN ARG          
SEQRES   8 B  112  LYS LEU GLU ARG HIS ARG GLN GLU GLN GLU LEU GLU LYS          
SEQRES   9 B  112  GLN HIS ARG GLU GLN LYS LEU GLN                              
SEQRES   1 C  112  GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY LEU          
SEQRES   2 C  112  VAL PRO ARG GLY SER HIS MET ALA GLU PRO ALA LEU ARG          
SEQRES   3 C  112  GLU GLN GLN LEU GLN GLN GLU LEU LEU ALA LEU LYS GLN          
SEQRES   4 C  112  LYS GLN GLN ILE GLN ARG GLN ILE LEU ILE ALA GLU PHE          
SEQRES   5 C  112  GLN ARG GLN HIS GLU GLN LEU SER ARG GLN HIS GLU ALA          
SEQRES   6 C  112  GLN LEU HIS GLU HIS ILE LYS GLN GLN GLN GLU MET LEU          
SEQRES   7 C  112  ALA MET LYS HIS GLN GLN GLU LEU LEU GLU HIS GLN ARG          
SEQRES   8 C  112  LYS LEU GLU ARG HIS ARG GLN GLU GLN GLU LEU GLU LYS          
SEQRES   9 C  112  GLN HIS ARG GLU GLN LYS LEU GLN                              
SEQRES   1 D  112  GLY SER SER HIS HIS HIS HIS HIS HIS SER SER GLY LEU          
SEQRES   2 D  112  VAL PRO ARG GLY SER HIS MET ALA GLU PRO ALA LEU ARG          
SEQRES   3 D  112  GLU GLN GLN LEU GLN GLN GLU LEU LEU ALA LEU LYS GLN          
SEQRES   4 D  112  LYS GLN GLN ILE GLN ARG GLN ILE LEU ILE ALA GLU PHE          
SEQRES   5 D  112  GLN ARG GLN HIS GLU GLN LEU SER ARG GLN HIS GLU ALA          
SEQRES   6 D  112  GLN LEU HIS GLU HIS ILE LYS GLN GLN GLN GLU MET LEU          
SEQRES   7 D  112  ALA MET LYS HIS GLN GLN GLU LEU LEU GLU HIS GLN ARG          
SEQRES   8 D  112  LYS LEU GLU ARG HIS ARG GLN GLU GLN GLU LEU GLU LYS          
SEQRES   9 D  112  GLN HIS ARG GLU GLN LYS LEU GLN                              
HELIX    1   1 GLU A   63  LYS A  122  1                                  60    
HELIX    2   2 GLN A  124  GLU A  129  5                                   6    
HELIX    3   3 GLU B   63  LYS B  122  1                                  60    
HELIX    4   4 GLN B  124  GLU B  129  5                                   6    
HELIX    5   5 GLU C   63  LYS C  122  1                                  60    
HELIX    6   6 GLN C  124  GLU C  129  5                                   6    
HELIX    7   7 GLU D   63  LYS D  122  1                                  60    
HELIX    8   8 GLN D  124  GLU D  129  5                                   6    
CRYST1  187.398   60.451   60.450  90.00 108.74  90.00 C 1 2 1      16          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.005336  0.000000  0.001810        0.00000                         
SCALE2      0.000000  0.016542  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.017469        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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