LOCUS       NP_011981                490 aa            linear   PLN 15-SEP-2023
DEFINITION  aspartyl aminopeptidase [Saccharomyces cerevisiae S288C].
ACCESSION   NP_011981
VERSION     NP_011981.1
DBLINK      BioProject: PRJNA128
DBSOURCE    REFSEQ: accession NM_001179243.1
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (residues 1 to 490)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (residues 1 to 490)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   3  (residues 1 to 490)
  AUTHORS   Johnston,M., Andrews,S., Brinkman,R., Cooper,J., Ding,H., Dover,J.,
            Du,Z., Favello,A., Fulton,L., Gattung,S. et al.
  TITLE     Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
            VIII
  JOURNAL   Science 265 (5181), 2077-2082 (1994)
   PUBMED   8091229
REFERENCE   4  (residues 1 to 490)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (residues 1 to 490)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Protein update by submitter
REFERENCE   6  (residues 1 to 490)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
  REMARK    Sequence update by submitter
REFERENCE   7  (residues 1 to 490)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA 94305-5120, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. The reference
            sequence is identical to DAA06807.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Saccharomyces cerevisiae S288C"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="VIII"
     Protein         1..490
                     /product="aspartyl aminopeptidase"
                     /EC_number="3.4.11.21"
                     /calculated_mol_wt=54043
     Region          20..479
                     /region_name="M18_DAP"
                     /note="M18 peptidase aspartyl aminopeptidase; cd05658"
                     /db_xref="CDD:349908"
     Site            order(97,273..274,308..309,362..363,365,390,397,430..431,
                     455..456)
                     /site_type="active"
                     /db_xref="CDD:349908"
     CDS             1..490
                     /gene="APE4"
                     /locus_tag="YHR113W"
                     /coded_by="NM_001179243.1:1..1473"
                     /experiment="EXISTENCE:direct assay:GO:0000328 fungal-type
                     vacuole lumen [PMID:21343297]"
                     /experiment="EXISTENCE:direct assay:GO:0004177
                     aminopeptidase activity [PMID:16367759]"
                     /experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
                     [PMID:11914276|PMID:14562095]"
                     /experiment="EXISTENCE:direct assay:GO:0006508 proteolysis
                     [PMID:15606766]"
                     /note="Cytoplasmic aspartyl aminopeptidase with possible
                     vacuole function; targeted to vacuole via Vps10p-dependent
                     endosomal vacuolar protein sorting pathway and via CVT
                     pathway; cleaves unblocked N-terminal acidic amino acids
                     from peptide substrates; forms 12-subunit homo-oligomer;
                     M18 metalloprotease family"
                     /db_xref="GeneID:856513"
                     /db_xref="SGD:S000001155"
ORIGIN      
        1 mfriqlrtms sktcksdypk efvsflnssh spyhtvhnik khlvsngfke lserdswagh
       61 vaqkgkyfvt rngssiiafa vggkwepgnp iaitgahtds palrikpisk rvsekylqvg
      121 vetyggaiwh swfdkdlgva grvfvkdakt gksiarlvdl nrpllkiptl aihldrdvnq
      181 kfefnretql lpigglqedk teaktekein ngeftsikti vqrhhaellg liakelaidt
      241 iediedfeli lydhnastlg gfndefvfsg rldnltscft smhgltlaad teidresgir
      301 lmacfdheei gsssaqgads nflpnilerl silkgdgsdq tkplfhsail etsaksffls
      361 sdvahavhpn yankyesqhk pllgggpvik inanqrymtn spglvlvkrl aeaakvplql
      421 fvvandspcg stigpilask tgirtldlgn pvlsmhsire tggsadlefq iklfkeffer
      481 ytsieseivv
//