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sce03030                    Pathway                                

DNA replication - Saccharomyces cerevisiae (budding yeast)
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
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DNA replication

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DNA polymerase alpha / primase complex [PATH:sce03030]
DNA polymerase epsilon complex [PATH:sce03030]
MCM complex [PATH:sce03030]
RF-C complex [PATH:sce03030]
Saccharomyces cerevisiae (budding yeast) [GN:sce]
RNH1; RNA-DNA hybrid ribonuclease [KO:K03469] [EC:]
RFA1; replication factor A subunit protein RFA1 [KO:K07466]
POL30; proliferating cell nuclear antigen [KO:K04802]
DNA2; bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2 [KO:K10742] [EC:]
RAD27; multifunctional nuclease RAD27 [KO:K04799] [EC:3.-.-.-]
CDC9; DNA ligase (ATP) CDC9 [KO:K10747] [EC:]
POL1; DNA-directed DNA polymerase alpha catalytic subunit POL1 [KO:K02320] [EC:]
POL12; DNA-directed DNA polymerase alpha subunit POL12 [KO:K02321]
PRI1; DNA primase subunit PRI1 [KO:K02684] [EC:2.7.7.-]
PRI2; DNA primase subunit PRI2 [KO:K02685] [EC:2.7.7.-]
POL3; DNA-directed DNA polymerase delta POL3 [KO:K02327] [EC:]
POL31; DNA-directed DNA polymerase delta subunit POL31 [KO:K02328]
POL32; DNA polymerase delta subunit POL32 [KO:K03504]
POL2; DNA polymerase epsilon catalytic subunit [KO:K02324] [EC:]
DPB2; DNA polymerase epsilon noncatalytic subunit [KO:K02325] [EC:]
DPB4; DNA polymerase epsilon noncatalytic subunit [KO:K02326] [EC:]
DPB3; DNA polymerase epsilon noncatalytic subunit [KO:K03506] [EC:]
DLS1; Dls1p [KO:K03506] [EC:]
MCM2; MCM DNA helicase complex subunit MCM2 [KO:K02540] [EC:]
MCM3; MCM DNA helicase complex subunit MCM3 [KO:K02541] [EC:]
MCM4; MCM DNA helicase complex subunit MCM4 [KO:K02212] [EC:]
MCM5; MCM DNA helicase complex subunit MCM5 [KO:K02209] [EC:]
MCM6; MCM DNA helicase complex subunit MCM6 [KO:K02542] [EC:]
MCM7; mini-chromosome maintenance complex protein 7 [KO:K02210] [EC:]
RFA2; Rfa2p [KO:K10739]
RFC1; replication factor C subunit 1 [KO:K10754]
RFC2; replication factor C subunit 2 [KO:K10755]
RFC4; replication factor C subunit 4 [KO:K10755]
RFC3; replication factor C subunit 3 [KO:K10756]
RFC5; replication factor C subunit 5 [KO:K10756]
RNH201; ribonuclease H2 catalytic subunit RNH201 [KO:K10743] [EC:]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
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