KEGG   PATHWAY: sce04120Help
sce04120                    Pathway                                

Ubiquitin mediated proteolysis - Saccharomyces cerevisiae (budding yeast)
Protein ubiquitination plays an important role in eukaryotic cellular processes. It mainly functions as a signal for 26S proteasome dependent protein degradation. The addition of ubiquitin to proteins being degraded is performed by a reaction cascade consisting of three enzymes, named E1 (ubiquitin activating enzyme), E2 (ubiquitin conjugating enzyme), and E3 (ubiquitin ligase). Each E3 has specificity to its substrate, or proteins to be targeted by ubiquitination. Many E3s are discovered in eukaryotes and they are classified into four types: HECT type, U-box type, single RING-finger type, and multi-subunit RING-finger type. Multi-subunit RING-finger E3s are exemplified by cullin-Rbx E3s and APC/C. They consist of a RING-finger-containing subunit (RBX1 or RBX2) that functions to bind E2s, a scaffold-like cullin molecule, adaptor proteins, and a target recognizing subunit that binds substrates.
Genetic Information Processing; Folding, sorting and degradation
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Pathway map
Ubiquitin mediated proteolysis

All organismsOrtholog table
SCF-MET30 complex [PATH:sce04120]
APC/C complex [PATH:sce04120]
SCF-CDC4 complex [PATH:sce04120]
SCF-GRR1 complex [PATH:sce04120]
Other DBs
Saccharomyces cerevisiae (budding yeast) [GN:sce]
UBA1; E1 ubiquitin-activating protein UBA1 [KO:K03178] [EC:]
AOS1; E1 ubiquitin-activating protein AOS1 [KO:K10684] [EC:]
UBA2; E1 ubiquitin-activating protein UBA2 [KO:K10685] [EC:]
UBA3; NEDD8-activating protein UBA3 [KO:K10686] [EC:]
RAD6; E2 ubiquitin-conjugating protein RAD6 [KO:K10573] [EC:]
UBC11; putative E2 ubiquitin-protein ligase UBC11 [KO:K06688] [EC:]
UBC5; E2 ubiquitin-conjugating protein UBC5 [KO:K06689] [EC:]
UBC4; E2 ubiquitin-conjugating protein UBC4 [KO:K06689] [EC:]
UBC7; E2 ubiquitin-conjugating protein UBC7 [KO:K04555] [EC:]
UBC8; E2 ubiquitin-conjugating protein UBC8 [KO:K10576] [EC:]
UBC9; E2 SUMO-conjugating protein UBC9 [KO:K10577]
UBC6; E2 ubiquitin-conjugating protein UBC6 [KO:K04554] [EC:]
UBC12; NEDD8-conjugating protein UBC12 [KO:K10579]
UBC13; E2 ubiquitin-conjugating protein UBC13 [KO:K10580] [EC:]
CDC34; SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 [KO:K02207] [EC:]
UBC1; E2 ubiquitin-conjugating protein UBC1 [KO:K04649] [EC:]
HUL5; ubiquitin-ubiquitin ligase HUL5 [KO:K10589] [EC:]
UFD4; putative ubiquitin-protein ligase UFD4 [KO:K10590] [EC:]
RSP5; NEDD4 family E3 ubiquitin-protein ligase [KO:K10591] [EC:]
TOM1; E3 ubiquitin-protein ligase TOM1 [KO:K10592] [EC:]
UFD2; ubiquitin-ubiquitin ligase UFD2 [KO:K10597] [EC:]
PRP19; E3 ubiquitin-protein ligase PRP19 [KO:K10599] [EC:]
SIZ1; SUMO ligase SIZ1 [KO:K04706]
HRD1; E3 ubiquitin-protein ligase HRD1 [KO:K10601] [EC:]
HRT1; SCF ubiquitin ligase complex subunit HRT1 [KO:K03868]
CDC53; cullin CDC53 [KO:K03347]
SKP1; SCF ubiquitin ligase subunit SKP1 [KO:K03094]
CDC4; SCF ubiquitin ligase complex subunit CDC4 [KO:K03361]
MET30; ubiquitin-binding SDF ubiquitin ligase complex subunit MET30 [KO:K10259]
GRR1; SCF ubiquitin ligase complex subunit GRR1 [KO:K03360]
ELC1; elongin C [KO:K03872]
CUL3; cullin CUL3 [KO:K03869]
RAD28; Rad28p [KO:K10570]
APC11; anaphase promoting complex subunit 11 [KO:K03358]
APC2; anaphase promoting complex subunit 2 [KO:K03349]
CDC20; ubiquitin-protein transferase activating protein CDC20 [KO:K03363]
CDH1; Cdh1p [KO:K03364]
APC1; anaphase promoting complex subunit 1 [KO:K03348]
CDC27; anaphase promoting complex subunit CDC27 [KO:K03350]
APC4; anaphase promoting complex subunit 4 [KO:K03351]
APC5; anaphase promoting complex subunit 5 [KO:K03352]
CDC16; anaphase promoting complex subunit CDC16 [KO:K03353]
CDC23; anaphase promoting complex subunit CDC23 [KO:K03355]
APC9; anaphase promoting complex subunit 9 [KO:K03356]
DOC1; anaphase promoting complex subunit DOC1 [KO:K03357]
CDC26; anaphase promoting complex subunit CDC26 [KO:K03359]
SWM1; Swm1p [KO:K12416]
PMID:16922370 (Japanese)
Kamura T.
[Cullin-based E3 family] Japanese
Tanpakushitsu Kakusan Koso 51:1167-72 (2006)
PMID:16922408 (Japanese)
Matsuda N.
[Overview: hereditary disorders caused by dysfunction of ubiquitylation]
Tanpakushitsu Kakusan Koso 51:1402-7 (2006)
PMID:16922367 (Japanese)
Tokunaga F.
[E2 ubiquitin-conjugating enzymes: structures and functions]
Tanpakushitsu Kakusan Koso 51:1150-6 (2006)
Nalepa G, Rolfe M, Harper JW.
Drug discovery in the ubiquitin-proteasome system.
Nat Rev Drug Discov 5:596-613 (2006)
Hsu YJ, Goodman SR.
Spectrin and ubiquitination: a review.
Cell Mol Biol (Noisy-le-grand) Suppl 51:OL801-7 (2005)
Hatakeyama S, Nakayama KI.
Ubiquitylation as a quality control system for intracellular proteins.
J Biochem 134:1-8 (2003)
Lee J, Zhou P.
DCAFs, the missing link of the CUL4-DDB1 ubiquitin ligase.
Mol Cell 26:775-80 (2007)
KO pathway

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