ID A0A061GYJ5_THECC Unreviewed; 527 AA.
AC A0A061GYJ5;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 24-JAN-2024, entry version 45.
DE RecName: Full=Alpha-1,3-glucosyltransferase {ECO:0000256|RuleBase:RU363110};
DE EC=2.4.1.- {ECO:0000256|RuleBase:RU363110};
GN ORFNames=TCM_039791 {ECO:0000313|EMBL:EOY32194.1};
OS Theobroma cacao (Cacao) (Cocoa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY32194.1, ECO:0000313|Proteomes:UP000026915};
RN [1] {ECO:0000313|EMBL:EOY32194.1, ECO:0000313|Proteomes:UP000026915}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA Kuhn D.N.;
RT "The genome sequence of the most widely cultivated cacao type and its use
RT to identify candidate genes regulating pod color.";
RL Genome Biol. 14:R53.1-R53.24(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a dolichyl beta-D-glucosyl phosphate + alpha-D-Glc-(1->3)-
CC alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-
CC Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-
CC (1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-
CC alpha-D-GlcNAc-diphosphodolichol = a dolichyl phosphate + alpha-D-
CC Glc-(1->3)-alpha-D-Glc-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-
CC alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-
CC Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-
CC beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + H(+);
CC Xref=Rhea:RHEA:31307, Rhea:RHEA-COMP:9517, Rhea:RHEA-COMP:9528,
CC Rhea:RHEA-COMP:12632, Rhea:RHEA-COMP:12633, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57525, ChEBI:CHEBI:57683, ChEBI:CHEBI:132521,
CC ChEBI:CHEBI:132522; EC=2.4.1.265;
CC Evidence={ECO:0000256|ARBA:ARBA00034048};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000256|ARBA:ARBA00004922, ECO:0000256|RuleBase:RU363110}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477, ECO:0000256|RuleBase:RU363110}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004477,
CC ECO:0000256|RuleBase:RU363110}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the ALG6/ALG8 glucosyltransferase family.
CC {ECO:0000256|ARBA:ARBA00008715, ECO:0000256|RuleBase:RU363110}.
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DR EMBL; CM001887; EOY32194.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A061GYJ5; -.
DR STRING; 3641.A0A061GYJ5; -.
DR EnsemblPlants; EOY32194; EOY32194; TCM_039791.
DR Gramene; EOY32194; EOY32194; TCM_039791.
DR eggNOG; KOG2576; Eukaryota.
DR HOGENOM; CLU_022045_2_0_1; -.
DR InParanoid; A0A061GYJ5; -.
DR OMA; YHSTDFD; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000026915; Chromosome 9.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0042283; F:dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006490; P:oligosaccharide-lipid intermediate biosynthetic process; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR InterPro; IPR004856; Glyco_trans_ALG6/ALG8.
DR PANTHER; PTHR12413; DOLICHYL GLYCOSYLTRANSFERASE; 1.
DR PANTHER; PTHR12413:SF2; DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED; 1.
DR Pfam; PF03155; Alg6_Alg8; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824,
KW ECO:0000256|RuleBase:RU363110};
KW Glycosyltransferase {ECO:0000256|RuleBase:RU363110};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363110};
KW Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363110};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363110};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363110}.
FT TRANSMEM 12..33
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 104..123
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 135..153
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 159..175
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 182..206
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 218..241
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 262..288
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 342..359
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 409..428
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 464..486
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
FT TRANSMEM 498..517
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363110"
SQ SEQUENCE 527 AA; 60078 MW; FBDC823A90D21B12 CRC64;
MAKHESRSMS IAQLLWYMGI ATTIKLLVIP SYHSTDLEVH RHWLAITHSL PLSQWYFDET
SQWTLDYPPF FAYFQRFLSV FAHLVDPQIV HLHHGLNYKA SSAIYFQRIS VIVSDLFFMY
GVYRCTSNLP SLKRNLVWIL AVWSPGLVIV DHLHFQYNGF LFGWLLLSIS FLAQGRDLMG
GFLFAVLLCF KHLFAVAAPV YFVYLLRHYC RGGLVKGFAR LSVMGAVVVA VFAAAYGPFV
YHGQIQQVIR RMFPFGRGLC HAYWAPNFWV FYIMLDKGIA FLLTKFGFNI PSPAASFTGG
LVGDASPFVI LPQITPMTTF ILVLLALSPC LIKTWMDPRP RLITRSVAYA YTCGFLFGWH
VHEKASLHFV IPLAIVAVES MEDAKHYFML SIVSSYSLFP LLYEAQEYPI KVLLLLLHSI
LIWFCFSAQF TKNKAMKSTL QADKKAAQFE LKGSSRTAAV NGGFVLGWLA KSYLFGVLVV
EIWGQFLHPY LLGDKFPFVP LMLISIYCAL GILYSWIWQL TQIMILS
//
