ID A0A061IAV5_CRIGR Unreviewed; 580 AA.
AC A0A061IAV5;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=medium-chain acyl-CoA ligase {ECO:0000256|ARBA:ARBA00039009};
DE EC=6.2.1.2 {ECO:0000256|ARBA:ARBA00039009};
GN Name=Acsm3 {ECO:0000313|RefSeq:XP_035298154.1};
GN ORFNames=H671_3g9170 {ECO:0000313|EMBL:ERE80005.1};
OS Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Cricetulus.
OX NCBI_TaxID=10029 {ECO:0000313|EMBL:ERE80005.1, ECO:0000313|Proteomes:UP000030759};
RN [1] {ECO:0000313|Proteomes:UP000030759}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=17A/GY {ECO:0000313|Proteomes:UP000030759};
RX PubMed=23929341; DOI=10.1038/nbt.2645;
RA Brinkrolf K., Rupp O., Laux H., Kollin F., Ernst W., Linke B., Kofler R.,
RA Romand S., Hesse F., Budach W.E., Galosy S., Muller D., Noll T.,
RA Wienberg J., Jostock T., Leonard M., Grillari J., Tauch A., Goesmann A.,
RA Helk B., Mott J.E., Puhler A., Borth N.;
RT "Chinese hamster genome sequenced from sorted chromosomes.";
RL Nat. Biotechnol. 31:694-695(2013).
RN [2] {ECO:0000313|EMBL:ERE80005.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=17A/GY {ECO:0000313|EMBL:ERE80005.1};
RA Brinkrolf K., Rupp O., Laux H., Kollin F., Ernst W., Linke B., Kofler R.,
RA Romand S., Hesse F., Budach W.E., Galosy S., Muller D., Noll T.,
RA Wienberg J., Jostock T., Leonard M., Grillari J., Tauch A., Goesmann A.,
RA Helk B., Mott J.E., Puehler A., Borth N.;
RT "Chinese hamster genome sequenced from sorted chromosomes.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|RefSeq:XP_035298154.1}
RP IDENTIFICATION.
RC STRAIN=17A/GY {ECO:0000313|RefSeq:XP_035298154.1};
RC TISSUE=Liver {ECO:0000313|RefSeq:XP_035298154.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a medium chain fatty acid + ATP + CoA = a medium-chain fatty
CC acyl-CoA + AMP + diphosphate; Xref=Rhea:RHEA:48340,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:59558, ChEBI:CHEBI:90546, ChEBI:CHEBI:456215; EC=6.2.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00036212};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:48341;
CC Evidence={ECO:0000256|ARBA:ARBA00036212};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000256|ARBA:ARBA00006432}.
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DR EMBL; KE671648; ERE80005.1; -; Genomic_DNA.
DR RefSeq; XP_007632846.1; XM_007634656.2.
DR RefSeq; XP_035298154.1; XM_035442263.1.
DR KEGG; cge:100751305; -.
DR OrthoDB; 5474118at2759; -.
DR Proteomes; UP000030759; Unassembled WGS sequence.
DR Proteomes; UP001108280; Chromosome 3.
DR GO; GO:0047760; F:butyrate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0031956; F:medium-chain fatty acid-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW.
DR CDD; cd05928; MACS_euk; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR PANTHER; PTHR43605; ACYL-COENZYME A SYNTHETASE; 1.
DR PANTHER; PTHR43605:SF7; ACYL-COENZYME A SYNTHETASE ACSM3, MITOCHONDRIAL; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:ERE80005.1};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 72..420
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 486..566
FT /note="AMP-binding enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF13193"
SQ SEQUENCE 580 AA; 65389 MW; D4B0E738E32AB5E7 CRC64;
MVMLLCAKYF QRLAIPGPLR VLYKDYGTAT PQNFSNYESM KRDFKLEIPE YFNFAKDVLD
QWSNVEKAGQ RLSNPAFWWV DGNGGEVRWG FEELGYLSRK FANILTEACS LQRGDRVMLI
LPKIPEWWLA NVACLRTGTV LIPGTTQLTQ KDILYRLQSS KAKCIITDDA LAPAVDTVAA
KCENLSSKLI VSQHSREGWG NLKEMMKYAS DNHICVNTKH NEMMAIYFTS GTTGPPKMIG
HTHSSFGLGL SVNGRFWLDL TDSDVMWNTS DTGWAKSSWS SVFSPWTQGA CVFVHHLPRF
ESTSILQTLS KFPITVFCSA PTAYRMLVQS DTTSYKFKSL QHCVSAGEPI NPEVMEQWRR
KTGLDIYEGY GQTETVLICG NFKGMKIKPG SMGKPSPAFN VKILDENGAI LPPGQEGDIG
IQVLPDRPFG LFTHYVDNPS KTASTLRGNF YITGDRGYMD EDGYFWFVAR SDDIILSSGY
RIGPFEVESA LIEHPSIAES AVVSSPDPIR GEVVKAFIVL NAEYKSHDQE QLKKEIQEHV
KRTTAPYKYP RKVEFIEELP KTVSGKVKRG ELRKKEWQTI
//
