ID A0A0B4S5T3_9BACI Unreviewed; 553 AA.
AC A0A0B4S5T3;
DT 01-APR-2015, integrated into UniProtKB/TrEMBL.
DT 01-APR-2015, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Glycerol-3-phosphate dehydrogenase {ECO:0000256|RuleBase:RU361217};
DE EC=1.1.5.3 {ECO:0000256|RuleBase:RU361217};
GN ORFNames=QR42_04610 {ECO:0000313|EMBL:AIZ59565.1};
OS Bacillus sp. WP8.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=756828 {ECO:0000313|EMBL:AIZ59565.1, ECO:0000313|Proteomes:UP000031306};
RN [1] {ECO:0000313|EMBL:AIZ59565.1, ECO:0000313|Proteomes:UP000031306}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WP8 {ECO:0000313|EMBL:AIZ59565.1,
RC ECO:0000313|Proteomes:UP000031306};
RX PubMed=25614565;
RA Kang Y., Shen M., Wang H., Zhao Q.;
RT "Complete Genome Sequence of Bacillus pumilus Strain WP8, an Efficient
RT Plant Growth-Promoting Rhizobacterium.";
RL Genome Announc. 3:0-0(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + sn-glycerol 3-phosphate = a quinol +
CC dihydroxyacetone phosphate; Xref=Rhea:RHEA:18977, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57642, ChEBI:CHEBI:132124; EC=1.1.5.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000153,
CC ECO:0000256|RuleBase:RU361217};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU361217};
CC -!- PATHWAY: Polyol metabolism; glycerol degradation via glycerol kinase
CC pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic
CC route): step 1/1. {ECO:0000256|ARBA:ARBA00004977}.
CC -!- SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate
CC dehydrogenase family. {ECO:0000256|ARBA:ARBA00007330,
CC ECO:0000256|RuleBase:RU361217}.
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DR EMBL; CP010075; AIZ59565.1; -; Genomic_DNA.
DR RefSeq; WP_039177448.1; NZ_CP010075.1.
DR AlphaFoldDB; A0A0B4S5T3; -.
DR KEGG; bacw:QR42_04610; -.
DR HOGENOM; CLU_015740_5_2_9; -.
DR UniPathway; UPA00618; UER00674.
DR Proteomes; UP000031306; Chromosome.
DR GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0004368; F:glycerol-3-phosphate dehydrogenase (quinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0019563; P:glycerol catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.8.870; Alpha-glycerophosphate oxidase, cap domain; 1.
DR Gene3D; 3.30.9.10; D-Amino Acid Oxidase, subunit A, domain 2; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR031656; DAO_C.
DR InterPro; IPR038299; DAO_C_sf.
DR InterPro; IPR006076; FAD-dep_OxRdtase.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000447; G3P_DH_FAD-dep.
DR PANTHER; PTHR11985:SF37; AEROBIC GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR PANTHER; PTHR11985; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; 1.
DR Pfam; PF01266; DAO; 1.
DR Pfam; PF16901; DAO_C; 1.
DR PRINTS; PR01001; FADG3PDH.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00977; FAD_G3PDH_1; 1.
DR PROSITE; PS00978; FAD_G3PDH_2; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU361217};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU361217}.
FT DOMAIN 21..376
FT /note="FAD dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF01266"
FT DOMAIN 403..531
FT /note="Alpha-glycerophosphate oxidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16901"
SQ SEQUENCE 553 AA; 61820 MW; FC9F4013F04109D5 CRC64;
MTFSSLEREQ MLQEMTKKPY DVFIIGGGIT GAGTALDAAS RGMRVGLAEM QDFAAGTSSR
STKLVHGGLR YLKQFEVKMV AEVGKERAIV YENGPHVTTP EWMLLPMHKG GTFGKFSTSI
GLRVYDFLAG VKHSERRSML SAKETLAKEP LVKKDGLKGG GYYVEYRTDD ARLTIEVMKE
AVKFGAEAVN YAKVKEFIYD KGKVVGVAIE DVMTKKTYDV YAKKIVNATG PWVDQLRDKD
HSKEGKHLQH TKGIHLVFDQ SVFPLKQAIY FDTPDKRMVF AIPREGKTYV GTTDTVYKKQ
LEHPRMTKAD RDYVIQAIQY MFPDLNITEK DVESNWAGLR PLIHEEGKDP SEISRKDEVW
TSSSGLITIA GGKLTGYRKM AEHIVDLVRD GLKQETGKDY GPCKTKHMPI SGGHVGGSKN
MAAFVQAKTA EGASVGLTEP IAQKLAEKYG SNVSSVYKRV EQLQGEADKR NIPAYVLAEL
VYAIEEELAV TPVDFFLRRT GSLLFNINWA KKYAQPVVDY MAERFGWDEA AKQKHQTELD
QLFHEAVVPL DAE
//
