ID A0A0P0FQC8_BACT4 Unreviewed; 481 AA.
AC A0A0P0FQC8; C6IQB1;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ALJ42831.1};
DE EC=3.2.1.1 {ECO:0000313|EMBL:ALJ42831.1};
GN ORFNames=BSIG_4048 {ECO:0000313|EMBL:EES66920.1}, Btheta7330_03297
GN {ECO:0000313|EMBL:ALJ42831.1}, DW780_23870
GN {ECO:0000313|EMBL:RHD81678.1}, GAC70_22160
GN {ECO:0000313|EMBL:KAB5117635.1}, GAO51_04735
GN {ECO:0000313|EMBL:KAB4315224.1};
OS Bacteroides thetaiotaomicron.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=818 {ECO:0000313|EMBL:ALJ42831.1, ECO:0000313|Proteomes:UP000066931};
RN [1] {ECO:0000313|EMBL:EES66920.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1_1_6 {ECO:0000313|EMBL:EES66920.1};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Allen-Vercoe E., Strauss J.,
RA Ambrose C., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Brown A.,
RA Chapman S.B., Chen Z., Dunbar C., Freedman E., Gearin G., Goldberg J.,
RA Griggs A., Gujja S., Heiman D., Howarth C., Larson L., Lui A.,
RA MacDonald P.J.P., Montmayeur A., Murphy C., Neiman D., Pearson M.,
RA Priest M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C.,
RA Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Bacteroides sp. 1_1_6.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ALJ42831.1, ECO:0000313|Proteomes:UP000066931}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=7330 {ECO:0000313|EMBL:ALJ42831.1,
RC ECO:0000313|Proteomes:UP000066931};
RX PubMed=26430127;
RA Wu M., McNulty N.P., Rodionov D.A., Khoroshkin M.S., Griffin N.W.,
RA Cheng J., Latreille P., Kerstetter R.A., Terrapon N., Henrissat B.,
RA Osterman A.L., Gordon J.I.;
RT "Genetic determinants of in vivo fitness and diet responsiveness in
RT multiple human gut Bacteroides.";
RL Science 350:AAC5992-AAC5992(2015).
RN [3] {ECO:0000313|EMBL:RHD81678.1, ECO:0000313|Proteomes:UP000284785}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AM30-26 {ECO:0000313|EMBL:RHD81678.1,
RC ECO:0000313|Proteomes:UP000284785};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|Proteomes:UP000440048, ECO:0000313|Proteomes:UP000440614}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIOML-A188 {ECO:0000313|EMBL:KAB4315224.1,
RC ECO:0000313|Proteomes:UP000440614}, and BIOML-A26
RC {ECO:0000313|EMBL:KAB5117635.1, ECO:0000313|Proteomes:UP000440048};
RX PubMed=31477907; DOI=.1038/s41591-019-0559-3;
RA Poyet M., Groussin M., Gibbons S.M., Avila-Pacheco J., Jiang X.,
RA Kearney S.M., Perrotta A.R., Berdy B., Zhao S., Lieberman T.D.,
RA Swanson P.K., Smith M., Roesemann S., Alexander J.E., Rich S.A., Livny J.,
RA Vlamakis H., Clish C., Bullock K., Deik A., Scott J., Pierce K.A.,
RA Xavier R.J., Alm E.J.;
RT "A library of human gut bacterial isolates paired with longitudinal
RT multiomics data enables mechanistic microbiome research.";
RL Nat. Med. 25:1442-1452(2019).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR EMBL; CP012937; ALJ42831.1; -; Genomic_DNA.
DR EMBL; ACIC02000066; EES66920.1; -; Genomic_DNA.
DR EMBL; WCSY01000003; KAB4315224.1; -; Genomic_DNA.
DR EMBL; WCMU01000090; KAB5117635.1; -; Genomic_DNA.
DR EMBL; QSJP01000030; RHD81678.1; -; Genomic_DNA.
DR RefSeq; WP_008766792.1; NZ_WCID01000007.1.
DR KEGG; btho:Btheta7330_03297; -.
DR PATRIC; fig|818.23.peg.3390; -.
DR HOGENOM; CLU_024572_2_0_10; -.
DR Proteomes; UP000003591; Unassembled WGS sequence.
DR Proteomes; UP000066931; Chromosome.
DR Proteomes; UP000284785; Unassembled WGS sequence.
DR Proteomes; UP000440048; Unassembled WGS sequence.
DR Proteomes; UP000440614; Unassembled WGS sequence.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Glycosidase {ECO:0000313|EMBL:ALJ42831.1};
KW Hydrolase {ECO:0000313|EMBL:ALJ42831.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT DOMAIN 4..410
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 232
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 262
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 103
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 182
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 195
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 201
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 203
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 236
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 481 AA; 55272 MW; B6BF20C6F0C90C90 CRC64;
MENGVMMQYF EWHLPNDGKL WKQIKEDALH LHDIGVTAVW IPPAYKADEQ QDEGYATYDL
YDLGEFDQKG TIRTKYGTKD ELKKMIDELH KYHIAVYLDV VLNHKAGGDF TEKFMVVEVD
PKERTKALGE PFEIQGWTGY SFHGRKDKHS DFKWHWYHFS GTGFDDAQKR SGVFQIQGEG
KAWSEGVDSE NGNYDFLLCN DIDLDHPEVV SELNRWGKWV SNELNLDGMR LDAIKHMKDQ
FVAQFLDAVR SERGNDFYAV GEYWNGDLEA LDAYIEAVGH KVNLFDVPLH YNMFQASQEG
KDYDLRDILK DTLVEHHPDL AVTIVDNHDT QRGSSLESNV GDWFKPLAYG LILLMKEGYP
CLFYGDYYGI KGEKSPHTRI IDILLDARRK YAYGDQIEYF DHPSTIGFIR TGDEEHNGSG
LVFLMSNDEA GSKIMSLGEK HKGEVWHEIT GSISEEITLD EEGNGEFSVE SRNLAVWVKK
D
//
