ID A0A1R3Y1F3_MYCBO Unreviewed; 3069 AA.
AC A0A1R3Y1F3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE SubName: Full=PROBABLE FATTY ACID SYNTHASE FAS (FATTY ACID SYNTHETASE) {ECO:0000313|EMBL:SIU01169.1};
GN Name=fas {ECO:0000313|EMBL:SIU01169.1};
GN OrderedLocusNames=BQ2027_MB2553C {ECO:0000313|EMBL:SIU01169.1};
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413 {ECO:0000313|EMBL:SIU01169.1, ECO:0000313|Proteomes:UP000001419};
RN [1] {ECO:0000313|Proteomes:UP000001419}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97 {ECO:0000313|Proteomes:UP000001419};
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2] {ECO:0000313|EMBL:SIU01169.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AF2122/97 {ECO:0000313|EMBL:SIU01169.1};
RA Malone K.M.;
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000001419}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97 {ECO:0000313|Proteomes:UP000001419};
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
RN [4] {ECO:0000313|EMBL:CAB0186801.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AF2122/97 {ECO:0000313|EMBL:CAB0186801.1};
RA Farrell D., Gordon V S.;
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:SIU01169.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AF2122/97 {ECO:0000313|EMBL:SIU01169.1};
RA Malone M K., Farrell D., Malone K.;
RL Submitted (APR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000256|ARBA:ARBA00005254}.
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DR EMBL; LR777660; CAB0186801.1; -; Genomic_DNA.
DR EMBL; LT708304; SIU01169.1; -; Genomic_DNA.
DR RefSeq; NP_856198.1; NC_002945.3.
DR RefSeq; WP_003901420.1; NC_002945.4.
DR SMR; A0A1R3Y1F3; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0005835; C:fatty acid synthase complex; IEA:InterPro.
DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:InterPro.
DR GO; GO:0004312; F:fatty acid synthase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd00828; elong_cond_enzymes; 1.
DR CDD; cd03447; FAS_MaoC; 1.
DR CDD; cd08950; KR_fFAS_SDR_c_like; 1.
DR Gene3D; 1.20.930.70; -; 1.
DR Gene3D; 3.30.70.2430; -; 1.
DR Gene3D; 3.30.70.3320; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.90.25.70; -; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 3.10.129.10; Hotdog Thioesterase; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR013565; Fas1/AflB-like_central.
DR InterPro; IPR047224; FAS_alpha_su_C.
DR InterPro; IPR003965; Fatty_acid_synthase.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR002539; MaoC-like_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR032088; SAT.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR10982:SF23; FATTY ACID SYNTHASE SUBUNIT ALPHA; 1.
DR PANTHER; PTHR10982; MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF18094; DNA_pol_B_N; 1.
DR Pfam; PF08354; Fas1-AflB-like_hel; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF01575; MaoC_dehydratas; 1.
DR Pfam; PF16073; SAT; 1.
DR PRINTS; PR01483; FASYNTHASE.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS52004; KS3_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000001419}.
FT DOMAIN 2535..2990
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1729..1768
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3069 AA; 326308 MW; 6855667940C025A2 CRC64;
MTIHEHDRVS ADRGGDSPHT THALVDRLMA GEPYAVAFGG QGSAWLETLE ELVSATGIET
ELATLVGEAE LLLDPVTDEL IVVRPIGFEP LQWVRALAAE DPVPSDKHLT SAAVSVPGVL
LTQIAATRAL ARQGMDLVAT PPVAMAGHSQ GVLAVEALKA GGARDVELFA LAQLIGAAGT
LVARRRGISV LGDRPPMVSV TNADPERIGR LLDEFAQDVR TVLPPVLSIR NGRRAVVITG
TPEQLSRFEL YCRQISEKEE ADRKNKVRGG DVFSPVFEPV QVEVGFHTPR LSDGIDIVAG
WAEKAGLDVA LARELADAIL IRKVDWVDEI TRVHAAGARW ILDLGPGDIL TRLTAPVIRG
LGIGIVPAAT RGGQRNLFTV GATPEVARAW SSYAPTVVRL PDGRVKLSTK FTRLTGRSPI
LLAGMTPTTV DAKIVAAAAN AGHWAELAGG GQVTEEIFGN RIEQMAGLLE PGRTYQFNAL
FLDPYLWKLQ VGGKRLVQKA RQSGAAIDGV VISAGIPDLD EAVELIDELG DIGISHVVFK
PGTIEQIRSV IRIATEVPTK PVIMHVEGGR AGGHHSWEDL DDLLLATYSE LRSRANITVC
VGGGIGTPRR AAEYLSGRWA QAYGFPLMPI DGILVGTAAM ATKESTTSPS VKRMLVDTQG
TDQWISAGKA QGGMASSRSQ LGADIHEIDN SASRCGRLLD EVAGDAEAVA ERRDEIIAAM
AKTAKPYFGD VADMTYLQWL RRYVELAIGE GNSTADTASV GSPWLADTWR DRFEQMLQRA
EARLHPQDFG PIQTLFTDAG LLDNPQQAIA ALLARYPDAE TVQLHPADVP FFVTLCKTLG
KPVNFVPVID QDVRRWWRSD SLWQAHDARY DADAVCIIPG TASVAGITRM DEPVGELLDR
FEQAAIDEVL GAGVEPKDVA SRRLGRADVA GPLAVVLDAP DVRWAGRTVT NPVHRIADPA
EWQVHDGPEN PRATHSSTGA RLQTHGDDVA LSVPVSGTWV DIRFTLPANT VDGGTPVIAT
EDATSAMRTV LAIAAGVDSP EFLPAVANGT ATLTVDWHPE RVADHTGVTA TFGEPLAPSL
TNVPDALVGP CWPAVFAAIG SAVTDTGEPV VEGLLSLVHL DHAARVVGQL PTVPAQLTVT
ATAANATDTD MGRVVPVSVV VTGADGAVIA TLEERFAILG RTGSAELADP ARAGGAVSAN
ATDTPRRRRR DVTITAPVDM RPFAVVSGDH NPIHTDRAAA LLAGLESPIV HGMWLSAAAQ
HAVTATDGQA RPPARLVGWT ARFLGMVRPG DEVDFRVERV GIDQGAEIVD VAARVGSDLV
MSASARLAAP KTVYAFPGQG IQHKGMGMEV RARSKAARKV WDTADKFTRD TLGFSVLHVV
RDNPTSIIAS GVHYHHPDGV LYLTQFTQVA MATVAAAQVA EMREQGAFVE GAIACGHSVG
EYTALACVTG IYQLEALLEM VFHRGSKMHD IVPRDELGRS NYRLAAIRPS QIDLDDADVP
AFVAGIAEST GEFLEIVNFN LRGSQYAIAG TVRGLEALEA EVERRRELTG GRRSFILVPG
IDVPFHSRVL RVGVAEFRRS LDRVMPRDAD PDLIIGRYIP NLVPRLFTLD RDFIQEIRDL
VPAEPLDEIL ADYDTWLRER PREMARTVFI ELLAWQFASP VRWIETQDLL FIEEAAGGLG
VERFVEIGVK SSPTVAGLAT NTLKLPEYAH STVEVLNAER DAAVLFATDT DPEPEPEEDE
PVAESPAPDV VSEAAPVAPA ASSAGPRPDD LVFDAADATL ALIALSAKMR IDQIEELDSI
ESITDGASSR RNQLLVDLGS ELNLGAIDGA AESDLAGLRS QVTKLARTYK PYGPVLSDAI
NDQLRTVLGP SGKRPGAIAE RVKKTWELGE GWAKHVTVEV ALGTREGSSV RGGAMGHLHE
GALADAASVD KVIDAAVASV AARQGVSVAL PSAGSGGGAT IDAAALSEFT DQITGREGVL
ASAARLVLGQ LGLDDPVNAL PAAPDSELID LVTAELGADW PRLVAPVFDP KKAVVFDDRW
ASAREDLVKL WLTDEGDIDA DWPRLAERFE GAGHVVATQA TWWQGKSLAA GRQIHASLYG
RIAAGAENPE PGRYGGEVAV VTGASKGSIA ASVVARLLDG GATVIATTSK LDEERLAFYR
TLYRDHARYG AALWLVAANM ASYSDVDALV EWIGTEQTES LGPQSIHIKD AQTPTLLFPF
AAPRVVGDLS EAGSRAEMEM KVLLWAVQRL IGGLSTIGAE RDIASRLHVV LPGSPNRGMF
GGDGAYGEAK SALDAVVSRW HAESSWAARV SLAHALIGWT RGTGLMGHND AIVAAVEEAG
VTTYSTDEMA ALLLDLCDAE SKVAAARSPI KADLTGGLAE ANLDMAELAA KAREQMSAAA
AVDEDAEAPG AIAALPSPPR GFTPAPPPQW DDLDVDPADL VVIVGGAEIG PYGSSRTRFE
MEVENELSAA GVLELAWTTG LIRWEDDPQP GWYDTESGEM VDESELVQRY HDAVVQRVGI
REFVDDGAID PDHASPLLVS VFLEKDFAFV VSSEADARAF VEFDPEHTVI RPVPDSTDWQ
VIRKAGTEIR VPRKTKLSRV VGGQIPTGFD PTVWGISADM AGSIDRLAVW NMVATVDAFL
SSGFSPAEVM RYVHPSLVAN TQGTGMGGGT SMQTMYHGNL LGRNKPNDIF QEVLPNIIAA
HVVQSYVGSY GAMIHPVAAC ATAAVSVEEG VDKIRLGKAQ LVVAGGLDDL TLEGIIGFGD
MAATADTSMM RGRGIHDSKF SRPNDRRRLG FVEAQGGGTI LLARGDLALR MGLPVLAVVA
FAQSFGDGVH TSIPAPGLGA LGAGRGGKDS PLARALAKLG VAADDVAVIS KHDTSTLAND
PNETELHERL ADALGRSEGA PLFVVSQKSL TGHAKGGAAV FQMMGLCQIL RDGVIPPNRS
LDCVDDELAG SAHFVWVRDT LRLGGKFPLK AGMLTSLGFG HVSGLVALVH PQAFIASLDP
AQRADYQRRA DARLLAGQRR LASAIAGGAP MYQRPGDRRF DHHAPERPQE ASMLLNPAAR
LGDGEAYIG
//
