ID A0A1S6HYE9_9GAMM Unreviewed; 877 AA.
AC A0A1S6HYE9;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595};
GN ORFNames=Sps_05424 {ECO:0000313|EMBL:AQS40492.1};
OS Shewanella psychrophila.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=225848 {ECO:0000313|EMBL:AQS40492.1, ECO:0000313|Proteomes:UP000189545};
RN [1] {ECO:0000313|EMBL:AQS40492.1, ECO:0000313|Proteomes:UP000189545}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WP2 {ECO:0000313|EMBL:AQS40492.1,
RC ECO:0000313|Proteomes:UP000189545};
RA Xu G., Jian H.;
RT "Complete genome sequence of Shewanella psychrophila WP2, a deep sea
RT bacterium isolated from west Pacific sediment.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
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DR EMBL; CP014782; AQS40492.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1S6HYE9; -.
DR STRING; 225848.Sps_05424; -.
DR KEGG; spsw:Sps_05424; -.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000189545; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:AQS40492.1}.
FT ACT_SITE 138
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 545
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 877 AA; 98923 MW; 6546E558B036F086 CRC64;
MTDMYASLRS NVGTLGQILG ETIRTNLDDA FLDKIEQIRQ LAKSSRQGDE AARDEMLKLL
TALPDDELVP FAKAFNQFLN LANIAEQFHT ISRNCDELVC VPDPVEQLLG RVLSSNIDQE
KMLDCLQNLD IDLVLTAHPT EISRRTLIQK YSSVIDSLAA LENPRLTERE KKQQHLRLRQ
LIAQIWHTNE IRHERPTPVD EARWGLSTIE VSLWQAIPDF LRQLNEQVEE RTGKQLPIDI
APVRFSSWMG GDRDGNPFVT AVVTQEVLDR NRHTAARLYL KDIVLLVNDL SMEEANNELS
ALTNNSHEPY RDVLRELRVK LRNTIDYLNE RLEGHNPEVE HSSIIWHEND LKEPLMMLYK
SLSDTGMSLI ANGLLLDMLR RIACFGIHML RLDIRQDAER HSDVIAELTR YLGMGDFDHW
DENEKQAFLL RELTSKRPLI PTNWQPSANV AEVISTCRLI ATQPAKALGS YVISMASKPS
DVLTVLLLLK ETGCPHPMRV VPLFETLDDL NNAATCMSAL FAIDWYRGYT KGHQEIMIGY
SDSAKDAGVM AAAWAQYHAQ EQLVEISRQA DVKLTLFHGR GGTIGRGGGP AHEAILSQPP
GSVDGRIRVT EQGEMIRFKF GLPKLAVQSL ALYTSAVMEA TLLPPPEPKP EWRACIQRLA
EESVKAYRSI VREEPDFVAY FRAATPEVEL GKLPLGSRPA KRKVDGGIES LRAIPWIFAW
SQNRLMLPAW LGAGEALQAS IDRGDMSLLQ EMEQEWPFFK TRISMLEMVY AKAEPNLAKY
YETCLVPENL HHLGEALRER LATGVKAVLE LTQSDTLMSH TPWNKESITL RNPYIDPLNF
MQAELLARTR KEDASANVEL ALMLTIAGVA AGMRNTG
//
