ID A7MKT1_CROS8 Unreviewed; 723 AA.
AC A7MKT1;
DT 02-OCT-2007, integrated into UniProtKB/TrEMBL.
DT 02-OCT-2007, sequence version 1.
DT 27-MAR-2024, entry version 78.
DE RecName: Full=Glycosyl hydrolase family 13 catalytic domain-containing protein {ECO:0000259|SMART:SM00642};
GN OrderedLocusNames=ESA_04156 {ECO:0000313|EMBL:ABU79337.1};
OS Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Cronobacter.
OX NCBI_TaxID=290339 {ECO:0000313|EMBL:ABU79337.1, ECO:0000313|Proteomes:UP000000260};
RN [1] {ECO:0000313|EMBL:ABU79337.1, ECO:0000313|Proteomes:UP000000260}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-894 {ECO:0000313|EMBL:ABU79337.1,
RC ECO:0000313|Proteomes:UP000000260};
RX PubMed=20221447; DOI=10.1371/journal.pone.0009556;
RA Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L.,
RA Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A.,
RA Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K.,
RA McClelland M., Forsythe S.J.;
RT "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic
RT hybridization analysis with other Cronobacter species.";
RL PLoS ONE 5:E9556-E9556(2010).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR036917-2};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR036917-2};
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DR EMBL; CP000783; ABU79337.1; -; Genomic_DNA.
DR AlphaFoldDB; A7MKT1; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; esa:ESA_04156; -.
DR HOGENOM; CLU_022115_1_0_6; -.
DR Proteomes; UP000000260; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR GO; GO:0009313; P:oligosaccharide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR014635; A_amylase_MalS.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10357:SF215; ALPHA-AMYLASE 1; 1.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR036917-2};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR036917-4};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036917-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000000260}.
FT DOMAIN 240..684
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 507
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT ACT_SITE 550
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT BINDING 361
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT BINDING 511
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT SITE 612
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-3"
FT DISULFID 103..121
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
FT DISULFID 168..584
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
SQ SEQUENCE 723 AA; 80853 MW; 96B5D83BE0B40E7A CRC64;
MPFLAFNAGV ETTRLSLPFP PLSLPLKDEE FLISPCDTEQ KSHRMHIMNL SPLALLLLPG
IALAQWSAPD LPAFQEKTPG VFISQGPLAK GTQPLRLTMD NACWQPAGPV KLNQILSLTP
CVDPAPEWRR FRDGNYKVQI DTRSGTPTLM LSVEQEAQKA VAEVTRQCPK WDGKSLTVSV
KETFPEGSLV RDFYSGNTAT VTQGQITLTP APGSNGLLLL ERADAASQAA FNWHNATVYF
VLTDRFVNGD PTNDNSYGRH KDGMQEIGTF HGGDLKGLTS KLDYLQTLGV NALWISSPLE
QIHGWVGGGT KGDFPHYAYH GYYPQDWTKL DANMGTPDDL RHLVDEAHRR GIRVLFDVVM
NHTGYATLAD MQQFQFGALY LKGDELKKTL GENWTDWRPG AGQNWHSFND YINFSDKAAW
EKWWGKTWIR TDIGDYDSPG FDDLTLSLTF LPDLKTESTQ PSGLPNFYQH KPDTAAQLID
GATPRDYLTR WLSQWVRDYG IDGFRVDTAK HVELAAWQQL KNEASAALVE WKAKNPDKKL
DDAPFWMTGE AWGHGVMQSD YYRHGFDAMI NFDYQEQAAK AADCFANIDM VWQQMAEKLQ
GFNALSYLSS HDTRLFREGG PRAAELLLLT PGAVQIFYGD ESERPFGPTG SDPIQGTRSD
MNWQDLNGKS AATLAHWQKL GQFRARHPAI GGGKQTTLSL QQGYGFIRQQ GDDTVMVVWA
GQQ
//
