ID B0WDK1_CULQU Unreviewed; 539 AA.
AC B0WDK1;
DT 08-APR-2008, integrated into UniProtKB/TrEMBL.
DT 08-APR-2008, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=Glucosylceramidase {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
DE EC=3.2.1.45 {ECO:0000256|ARBA:ARBA00012658, ECO:0000256|RuleBase:RU361188};
GN Name=6036791 {ECO:0000313|EnsemblMetazoa:CPIJ005343-PA};
GN ORFNames=CpipJ_CPIJ005343 {ECO:0000313|EMBL:EDS44610.1};
OS Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Culicini; Culex; Culex.
OX NCBI_TaxID=7176;
RN [1] {ECO:0000313|EMBL:EDS44610.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JHB {ECO:0000313|EMBL:EDS44610.1};
RG The Broad Institute Genome Sequencing Platform;
RA Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.,
RA Hannick L., Megy K., O'Leary S., Pearson M., Haas B.J., Mauceli E.,
RA Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA Fraser-Liggett C., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT "Annotation of Culex pipiens quinquefasciatus.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblMetazoa:CPIJ005343-PA}
RP IDENTIFICATION.
RC STRAIN=JHB {ECO:0000313|EnsemblMetazoa:CPIJ005343-PA};
RG EnsemblMetazoa;
RL Submitted (MAY-2020) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|RuleBase:RU361188};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 30 family.
CC {ECO:0000256|ARBA:ARBA00005382, ECO:0000256|RuleBase:RU361188}.
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DR EMBL; DS231898; EDS44610.1; -; Genomic_DNA.
DR RefSeq; XP_001846785.1; XM_001846733.1.
DR AlphaFoldDB; B0WDK1; -.
DR STRING; 7176.B0WDK1; -.
DR EnsemblMetazoa; CPIJ005343-RA; CPIJ005343-PA; CPIJ005343.
DR KEGG; cqu:CpipJ_CPIJ005343; -.
DR VEuPathDB; VectorBase:CPIJ005343; -.
DR eggNOG; KOG2566; Eukaryota.
DR HOGENOM; CLU_014379_1_2_1; -.
DR InParanoid; B0WDK1; -.
DR OMA; GLMWNFA; -.
DR Proteomes; UP000002320; Unassembled WGS sequence.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006665; P:sphingolipid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR033452; GH30_C.
DR InterPro; IPR001139; Glyco_hydro_30.
DR InterPro; IPR033453; Glyco_hydro_30_TIM-barrel.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR11069; GLUCOSYLCERAMIDASE; 1.
DR PANTHER; PTHR11069:SF23; LYSOSOMAL ACID GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF02055; Glyco_hydro_30; 1.
DR Pfam; PF17189; Glyco_hydro_30C; 1.
DR PRINTS; PR00843; GLHYDRLASE30.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361188};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361188};
KW Lipid metabolism {ECO:0000256|RuleBase:RU361188};
KW Reference proteome {ECO:0000313|Proteomes:UP000002320};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Sphingolipid metabolism {ECO:0000256|RuleBase:RU361188}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..539
FT /note="Glucosylceramidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011407885"
FT DOMAIN 120..472
FT /note="Glycosyl hydrolase family 30 TIM-barrel"
FT /evidence="ECO:0000259|Pfam:PF02055"
FT DOMAIN 475..536
FT /note="Glycosyl hydrolase family 30 beta sandwich"
FT /evidence="ECO:0000259|Pfam:PF17189"
SQ SEQUENCE 539 AA; 60502 MW; 09BC442FA951BEF3 CRC64;
MPHLNLIAAC ALLWAAGSAS LTAGASLPCA LRQYPSGSVC VCNATYCDTL EFEEPKVAGE
FVLVSSGRDG ARFREARGRF VNASRVEFLP VVMRTNGRRV VRATREVTVT VNRGRKFQRV
VGFGGAFTGA VAYNLGLLKP ELRKSLYRSY YSKKVGIGYN LMRIPIGGCD FDLKPWAYNE
VPVDDVGLSN FTKLDDRDVV KIAQIKELME VAENRNVKIM GAAWSPPRWM KSNNDWSGSS
RLKTEYFQTW ADYHIRYLQL MKDAGLDYWA ISTGNEPMNA VIGFLFIRFM SLGWTAIEQG
KWVGENLGPA LRNSAFKDVK LFAGDDQRYT FPWWFSQMDQ GHANATQYLD GLAVHWYWDG
VTPAALLDQA AKQYPDKLLF NTEASLGDKP FQTHGPILGS WDRAESYITY ILQDLQHSVN
GWIDWNLLLN EIGGPNYANN YVETAVVVNA TSGEEVYKQP IFYGLGHFSR FITEGSMRLE
TISSDSGVIV VGFERSDKLT TLVFYNKKST PCELTIKDPD RGTTQLRLEP KSVHSILYA
//
