ID E3EBZ3_PAEPS Unreviewed; 497 AA.
AC E3EBZ3;
DT 11-JAN-2011, integrated into UniProtKB/TrEMBL.
DT 11-JAN-2011, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ADO59117.1};
GN Name=amyL {ECO:0000313|EMBL:ADO59117.1};
GN ORFNames=PPSC2_24270 {ECO:0000313|EMBL:ADO59117.1};
OS Paenibacillus polymyxa (strain SC2) (Bacillus polymyxa).
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=886882 {ECO:0000313|EMBL:ADO59117.1, ECO:0000313|Proteomes:UP000006868};
RN [1] {ECO:0000313|EMBL:ADO59117.1, ECO:0000313|Proteomes:UP000006868}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC2 {ECO:0000313|EMBL:ADO59117.1,
RC ECO:0000313|Proteomes:UP000006868};
RX PubMed=21037012; DOI=10.1128/JB.01234-10;
RA Ma M., Wang C., Ding Y., Li L., Shen D., Jiang X., Guan D., Cao F.,
RA Chen H., Feng R., Wang X., Ge Y., Yao L., Bing X., Yang X., Li J., Du B.;
RT "Complete genome sequence of Paenibacillus polymyxa SC2, a strain of plant
RT growth-promoting Rhizobacterium with broad-spectrum antimicrobial
RT activity.";
RL J. Bacteriol. 193:311-312(2011).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR EMBL; CP002213; ADO59117.1; -; Genomic_DNA.
DR RefSeq; WP_013373652.1; NC_014622.2.
DR AlphaFoldDB; E3EBZ3; -.
DR SMR; E3EBZ3; -.
DR STRING; 1406.LK13_11790; -.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; ppm:PPSC2_24270; -.
DR PATRIC; fig|886882.15.peg.5139; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_024572_2_0_9; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000006868; Chromosome.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000006868}.
FT DOMAIN 5..391
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 233
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 263
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 104
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 196
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 204
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 237
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 497 AA; 57151 MW; 89B4CDF2DB1A97FA CRC64;
MKRNHTMMQF FEWNVKADGS HWKKLARLAP ELKAKGIDAI WIPPVTKGQS PEDTGYGVYD
LYDLGEFDQK GAVRTKYGTR EDLLEAVASC VRHGVAVYVD LVMNHKAGAD ETEVFKVVEV
NPDNRNEVIS EPFDIEGWTK FTFPGRQGKY STFQWNFEHF NGTDYDASQD RTGIYRILGK
NKNWSDNVDD EFGNYDYLMF ANIDYNHQDV RKEMIRWGKW LVDTLQCNGF RLDAIKHINH
EFVREFATEM IKKRGQDFYM VGEFWKPDLE SCQKFLDTID YKIDLFDVSL HYKLHSASLG
GKDFDLSTIF EDTLVHTHPL NSVTFVDNHD SQPHEALESW VEDWFKPSAY ALILLRKDGY
PCVFYGDYYG IQGQTPVEGK QAELDPLLYA RYHKAYGEQK DYFDDPHTIG WVRQGIPELH
GSGCAVVITN ANDGEKRMFV GKQRAGEKWT DLTGHHDHTI KIEQDGYGIF PVRAGSVSVW
ALPTEEDSLD AEDTASA
//
