ID   K8YU91_NANGC            Unreviewed;       607 AA.
AC   K8YU91;
DT   06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2013, sequence version 1.
DT   24-JAN-2024, entry version 38.
DE   RecName: Full=Acetyl-coenzyme A synthetase {ECO:0000256|RuleBase:RU361147};
DE            EC=6.2.1.1 {ECO:0000256|RuleBase:RU361147};
GN   Name=ACSS {ECO:0000313|EMBL:EKU22239.1};
GN   ORFNames=NGA_0216600 {ECO:0000313|EMBL:EKU22239.1};
OS   Nannochloropsis gaditana (strain CCMP526) (Green microalga)
OS   (Microchloropsis gaditana).
OC   Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae;
OC   Eustigmatales; Monodopsidaceae; Nannochloropsis.
OX   NCBI_TaxID=1093141 {ECO:0000313|EMBL:EKU22239.1, ECO:0000313|Proteomes:UP000009377};
RN   [1] {ECO:0000313|Proteomes:UP000009377}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP526 {ECO:0000313|Proteomes:UP000009377};
RX   PubMed=22353717;
RA   Radakovits R., Jinkerson R.E., Fuerstenberg S.I., Tae H., Settlage R.E.,
RA   Boore J.L., Posewitz M.C.;
RT   "Draft genome sequence and genetic transformation of the oleaginous alga
RT   Nannochloropis gaditana.";
RL   Nat. Commun. 3:686-686(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate;
CC         Xref=Rhea:RHEA:23176, ChEBI:CHEBI:30089, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:456215; EC=6.2.1.1;
CC         Evidence={ECO:0000256|RuleBase:RU361147};
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000256|RuleBase:RU361147}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JH471199; EKU22239.1; -; Genomic_DNA.
DR   RefSeq; XP_005854118.1; XM_005854056.1.
DR   AlphaFoldDB; K8YU91; -.
DR   EnsemblProtists; EKU22239; EKU22239; NGA_0216600.
DR   GeneID; 17360891; -.
DR   KEGG; ngd:NGA_0216600; -.
DR   OMA; TVHTKKI; -.
DR   OrthoDB; 144557at2759; -.
DR   Proteomes; UP000009377; Unassembled WGS sequence.
DR   GO; GO:0003987; F:acetate-CoA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016208; F:AMP binding; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019427; P:acetyl-CoA biosynthetic process from acetate; IEA:InterPro.
DR   CDD; cd05966; ACS; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR011904; Ac_CoA_lig.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   NCBIfam; TIGR02188; Ac_CoA_lig_AcsA; 1.
DR   PANTHER; PTHR24095; ACETYL-COENZYME A SYNTHETASE; 1.
DR   PANTHER; PTHR24095:SF14; ACETYL-COENZYME A SYNTHETASE 1; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU361147};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU361147};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU361147};
KW   Reference proteome {ECO:0000313|Proteomes:UP000009377}.
FT   DOMAIN          43..425
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   DOMAIN          486..564
FT                   /note="AMP-binding enzyme C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF13193"
SQ   SEQUENCE   607 AA;  66826 MW;  CD0D54030966F174 CRC64;
     MQAMWFSDFD RVSQGSFIEG DVAWFLNGKL NASVCCIDQH LPHRQDQVAI LWEGDEPGHC
     RSITYKELYR DVCRLANAMK AMGVRRGDVV MLYLPMVPEV VMAMLACARI GAPHSVVFAG
     FSAEALRDRI NDTNSKWVMT TDEGKRGGKT LPLKQIVDKA LKEAPSVQHC FVFERTGAPV
     DWVPGRDVKM SEVMAQQRPY CPAETMDSED LLFILYTSGS TGKPKGVAHT TAGYLLFAAM
     THKTVFDLQE GDVYACVADC GWITGHSYIV YGPLVNGATT LLFESIPTYP NHCRYWDLIQ
     RHRVTQFYTA PTAIRALMRY GTEDIASFDR SSLRVLGSVG EPINPEAWKW YFEHVGNARC
     TIVDTWWQTE TGGHLASPLP GVTPMKPGSC TLPFYGVELA LIDPSTGQEL AGNDVEGVLC
     VKRPWPSMTR TVFGDHARYL TVYMQPYKGY YFTGDGARRD ADGYYWITGR VDDVLNTSGH
     RIGTAEVESA LVGHAAVSEA AVVGFPHAVK GEGICCFVTL REGVFESPAL EKELKQAVRD
     AIGAFATPDI VCPTPSLPKT RSGKIMRRVL RKIASGESDS LGDVSTLADP SVVPYLITAI
     ALKVKTA
//