ID A0A045JWZ8_MYCTX Unreviewed; 1232 AA.
AC A0A045JWZ8;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN Name=narG {ECO:0000313|EMBL:SGI56594.1};
GN ORFNames=A4S10_01240 {ECO:0000313|EMBL:OMH59077.1}, ERS053720_03559
GN {ECO:0000313|EMBL:CFH95222.1}, GJE03_06160
GN {ECO:0000313|EMBL:QGK78422.1}, SAMEA2683035_03871
GN {ECO:0000313|EMBL:SGI56594.1};
OS Mycobacterium tuberculosis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=1773 {ECO:0000313|EMBL:SGI56594.1, ECO:0000313|Proteomes:UP000182946};
RN [1] {ECO:0000313|EMBL:CFH95222.1, ECO:0000313|Proteomes:UP000046674}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=0351H {ECO:0000313|EMBL:CFH95222.1,
RC ECO:0000313|Proteomes:UP000046674};
RG Pathogen Informatics;
RA Murphy D.;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OMH59077.1, ECO:0000313|Proteomes:UP000189452}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=6548 {ECO:0000313|EMBL:OMH59077.1,
RC ECO:0000313|Proteomes:UP000189452};
RA Bigi M., Bigi F., Soria M.A.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SGI56594.1, ECO:0000313|Proteomes:UP000182946}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2926STDY5723788 {ECO:0000313|EMBL:SGI56594.1,
RC ECO:0000313|Proteomes:UP000182946};
RG Pathogen Informatics;
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:OMH59077.1, ECO:0000313|Proteomes:UP000189452}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=6548 {ECO:0000313|EMBL:OMH59077.1,
RC ECO:0000313|Proteomes:UP000189452};
RA Bigi M.M., Lopez B., Blanco F.C., Sasiain M.C., De La Barrera S.,
RA Ritacco V., Bigi F., Soria M.A.;
RT "Protein polymorphisms may explain contrasting epidemiological fitness of
RT two variants of a multidrug-resistant Mycobacterium tuberculosis strain.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:QGK78422.1, ECO:0000313|Proteomes:UP000388593}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AUSMDU00018547 {ECO:0000313|EMBL:QGK78422.1,
RC ECO:0000313|Proteomes:UP000388593};
RA Baines S.L., Goncalves da Silva A., Carter G.P., Jennison A.V.,
RA Rathnayake I.U., Graham R.M.A., Sintchenko V., Wang Q., Rockett R.J.,
RA Timms V.J., Martinez E., Ballard S., Tomita T., Isles N., Horan K.A.,
RA Pitchers W., Stinear T.P., Williamson D.A., Howden B.P., Seemann T.;
RT "Complete microbial genomes for public health in Australia and Southwest
RT Pacific.";
RL Submitted (NOV-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR EMBL; CFSW01000028; CFH95222.1; -; Genomic_DNA.
DR EMBL; LWDQ01000001; OMH59077.1; -; Genomic_DNA.
DR EMBL; CP045962; QGK78422.1; -; Genomic_DNA.
DR EMBL; FPTZ01000042; SGI56594.1; -; Genomic_DNA.
DR RefSeq; WP_003898748.1; NZ_WUCS01000023.1.
DR AlphaFoldDB; A0A045JWZ8; -.
DR PATRIC; fig|1773.206.peg.814; -.
DR OMA; VWMSEAD; -.
DR Proteomes; UP000046674; Unassembled WGS sequence.
DR Proteomes; UP000182946; Unassembled WGS sequence.
DR Proteomes; UP000189452; Chromosome.
DR Proteomes; UP000388593; Chromosome.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:SGI56594.1}.
FT DOMAIN 53..117
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1232 AA; 136956 MW; F12194DA383644F9 CRC64;
MTVTPHVGGP LEELLERSGR FFTPGEFSAD LRTVTRRGGR EGDVFYRDRW SHDKVVRSTH
GVNCTGSCSW KIYVKDGIIT WETQQTDYPS VGPDRPEYEP RGCPRGASFS WYSYSPTRVR
YPYARGVLVE MYREAKTRLG DPVLAWADIQ ADPERRRRYQ QARGKGGLVR VSWAEASEMV
AAAHVHTIKT YGPDRVAGFS PIPAMSMVSH AAGSRFVELI GGVMTSFYDW YADLPVASPQ
VFGDQTDVPE SGDWWDASYL VMWGSNVPIT RTPDAHWMAE ARYRGAKVVV VSPDYADNTK
FADEWVRCAA GTDTALAMAM GHVILSECYV RNQVPFFVDY VRRYTDLPFL IKLEKRGDLL
VPGKFLTAAD IGEESENAAF KPALLDELTN TVVVPQGSLG FRFGEDGVGK WNLDLGSVVP
ALSVEMDKAV NGDRSAELVT LPSFDTIDGH GETVSRGVPV RRAGKHLVCT VFDLMLAHYG
VARAGLPGEW PTGYHDRTQQ NTPAWQESIT GVPAAQAIRF AKEFARNATE SGGRSMIIMG
GGICHWFHSD VMYRSVLALL MLTGSMGRNG GGWAHYVGQE KVRPLTGWQT MAMATDWSRP
PRQVPGASYW YAHTDQWRYD GYGADKLASP VGRGRFAGKH TMDLLTSATA MGWSPFYPQF
DRSSLDVADE ARAAGRDVGD YVAEQLAQHK LKLSITDPDN PVNWPRVLTV WRANLIGSSG
KGGEYFLRHL LGTDSNVQSD PPTDGVHPRD VVWDSDIPEG KLDLIMSIDF RMTSTTLVSD
VVLPAATWYE KSDLSSTDMH PYVHSFSPAI DPPWETRSDF DAFAAIARAF SALAKRHLGT
RTDVVLTALQ HDTPDEMAYP DGTERDWLAT GEVPVPGRTM SKLTVVERDY TAIYDKWLTL
GPLIDQFGMT TKGYTVHPFR EVSELAANFG VMNSGVAVGR PAITTAKRMA DVILALSGTC
NGRLAVEGFL ELEKRTGQRL AHLAEGSEER RITYADTQAR PVPVITSPEW SGSESGGRRY
APFTINIEHL KPFHTLTGRM HFYLAHDWVE ELGEQLPVYR PPLDMARLFN QPELGPTDDG
LGLTVRYLTP HSKWSFHSTY QDNLYMLSLS RGGPTMWMSP GDAAKINVRD NDWVEAVNAN
GIYVCRAIVS HRMPEGVVFV YHVQERTVDT PRTETNGKRG GNHNALTRVR IKPSHLAGGY
GQHAFAFNYL GPTGNQRDEV TVVRRRSQEV RY
//
