ID   A0A059BH65_EUCGR        Unreviewed;       919 AA.
AC   A0A059BH65;
DT   09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT   09-JUL-2014, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE            EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
DE   AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000256|ARBA:ARBA00030238};
GN   ORFNames=EUGRSUZ_G02543 {ECO:0000313|EMBL:KCW65005.1};
OS   Eucalyptus grandis (Flooded gum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX   NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW65005.1};
RN   [1] {ECO:0000313|EMBL:KCW65005.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW65005.1};
RA   Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT   "The genome of Eucalyptus grandis.";
RL   Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
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DR   EMBL; KK198759; KCW65005.1; -; Genomic_DNA.
DR   RefSeq; XP_010066950.1; XM_010068648.2.
DR   AlphaFoldDB; A0A059BH65; -.
DR   EnsemblPlants; KCW65005; KCW65005; EUGRSUZ_G02543.
DR   GeneID; 104453978; -.
DR   Gramene; KCW65005; KCW65005; EUGRSUZ_G02543.
DR   KEGG; egr:104453978; -.
DR   eggNOG; KOG0471; Eukaryota.
DR   OrthoDB; 201664at2759; -.
DR   GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR012850; A-amylase_bs_C.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF33; ALPHA-AMYLASE 3, CHLOROPLASTIC; 1.
DR   Pfam; PF07821; Alpha-amyl_C2; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SMART; SM00810; Alpha-amyl_C2; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
FT   DOMAIN          528..857
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   DOMAIN          858..918
FT                   /note="Alpha-amylase C-terminal beta-sheet"
FT                   /evidence="ECO:0000259|SMART:SM00810"
FT   REGION          21..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          421..443
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   919 AA;  103331 MW;  D7E88CD1D34E6DA2 CRC64;
     MSSAVSTEPL LHRSFREISR PRFRSSTLRP SPPPASLSCA PKSPSFRHAR RRCASGSGPR
     RGGWGGAGAG ALLRVRASSS GAAVVEALES ADVLFQEAFP LQRTETAKGK IFVRLDQGKD
     GQNWQLTVGC TLAGKWILHW GISHVDDVGS EWDQPPAEMR PPGSIPIKDY AIETPLQKSS
     TSMDGDEVYE VNISIKSNSN IAAINFVLKD EESGSWYQHR GRDFKVPLMD SLQDDSNIVG
     SKRGFDLWPG ALGQLSNILL KGEASDYKDK DASTDLEASK QEVRCLEGFY VEQPIVKVVS
     IRNSVNISVK ICPKTAKTLV YLETDLPRDV IVHWGVCRDD SKKWEIPAAP YPPETEIFKN
     KALRTLLQPK EQGNGFWGSF ALDEEFVGFL FVLKLKEEDV WLNCMGEDFY IPVSSTRSSS
     LIRQKESDST ETSGKTMETN TEVSSTAYTD DIINEIRNLV SDISSEKSRK TKTKEAQESI
     LQEIEKLAAE AYSIFRSSLP TFSAEAVLEQ EALEPPPHIC SGTGTGFEIL CQGFNWESNK
     SGRWYKELME KASQLASLGF TVVWLPPPTD SVSPEGYMPR DLYNLNSRYG TIDELKDLVK
     KFHEVNIRVL GDVVLNHRCA QYQNQNGIWN IFGGRLNWDD RAVVADDPHF QGRGNKSSGD
     NFHAAPNIDH SQDFVRKDLK EWLHWLRSEI GYDGWRLDFV RGFWGGYVKD YLDASEPYFA
     VGEYWDSLSY TYGEMDHNQD AHRQRIIDWI NATNGTAGAF DVTTKGILHA ALERCEYWRL
     SDQKGKPPGV VGWWPSRAVT FVENHDTGST QGHWRFPSGK EMQGYAYILT HPGTPAVFYD
     HIFSHYQSEI GSLISIRNRN KIHCRSTIKI TKAERDVYAA IIDDKVAMKI GPGYYEPQSG
     PQKWSPVLEG RDYKVWETS
//