ID A0A059BL04_EUCGR Unreviewed; 2265 AA.
AC A0A059BL04;
DT 09-JUL-2014, integrated into UniProtKB/TrEMBL.
DT 09-JUL-2014, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KCW66724.1};
GN ORFNames=EUGRSUZ_F00484 {ECO:0000313|EMBL:KCW66724.1};
OS Eucalyptus grandis (Flooded gum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71139 {ECO:0000313|EMBL:KCW66724.1};
RN [1] {ECO:0000313|EMBL:KCW66724.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf extractions {ECO:0000313|EMBL:KCW66724.1};
RA Schmutz J., Hayes R., Myburg A., Tuskan G., Grattapaglia D., Rokhsar D.S.;
RT "The genome of Eucalyptus grandis.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] =
CC ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate;
CC Xref=Rhea:RHEA:13501, Rhea:RHEA-COMP:10505, Rhea:RHEA-COMP:10506,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17544, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:83144, ChEBI:CHEBI:83145,
CC ChEBI:CHEBI:456216; EC=6.3.4.14;
CC Evidence={ECO:0000256|ARBA:ARBA00000861};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + ATP + hydrogencarbonate = ADP + H(+) + malonyl-
CC CoA + phosphate; Xref=Rhea:RHEA:11308, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:456216; EC=6.4.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001455};
CC -!- COFACTOR:
CC Name=biotin; Xref=ChEBI:CHEBI:57586;
CC Evidence={ECO:0000256|ARBA:ARBA00001953};
CC -!- PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from
CC acetyl-CoA: step 1/1. {ECO:0000256|ARBA:ARBA00004956}.
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DR EMBL; KK198758; KCW66724.1; -; Genomic_DNA.
DR RefSeq; XP_010060156.1; XM_010061854.2.
DR STRING; 71139.A0A059BL04; -.
DR EnsemblPlants; KCW66724; KCW66724; EUGRSUZ_F00484.
DR GeneID; 104448070; -.
DR Gramene; KCW66724; KCW66724; EUGRSUZ_F00484.
DR KEGG; egr:104448070; -.
DR eggNOG; KOG0368; Eukaryota.
DR InParanoid; A0A059BL04; -.
DR OMA; PTPKGHC; -.
DR OrthoDB; 911at2759; -.
DR UniPathway; UPA00655; UER00711.
DR GO; GO:0003989; F:acetyl-CoA carboxylase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IBA:GO_Central.
DR GO; GO:2001295; P:malonyl-CoA biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd06850; biotinyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.30.1490.20; ATP-grasp fold, A domain; 1.
DR Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 1.
DR Gene3D; 2.40.460.10; Biotin dependent carboxylase carboxyltransferase; 1.
DR Gene3D; 3.90.1770.10; PreATP-grasp domain; 1.
DR InterPro; IPR049076; ACCA.
DR InterPro; IPR049074; ACCA_BT.
DR InterPro; IPR034733; AcCoA_carboxyl_beta.
DR InterPro; IPR013537; AcCoA_COase_cen.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR005481; BC-like_N.
DR InterPro; IPR001882; Biotin_BS.
DR InterPro; IPR011764; Biotin_carboxylation_dom.
DR InterPro; IPR005482; Biotin_COase_C.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR005479; CbamoylP_synth_lsu-like_ATP-bd.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR011763; COA_CT_C.
DR InterPro; IPR011762; COA_CT_N.
DR InterPro; IPR016185; PreATP-grasp_dom_sf.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR45728:SF3; ACETYL-COA CARBOXYLASE; 1.
DR PANTHER; PTHR45728; ACETYL-COA CARBOXYLASE, ISOFORM A; 1.
DR Pfam; PF08326; ACC_central; 1.
DR Pfam; PF21385; ACCA_BT; 1.
DR Pfam; PF02785; Biotin_carb_C; 1.
DR Pfam; PF00289; Biotin_carb_N; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF01039; Carboxyl_trans; 1.
DR Pfam; PF02786; CPSase_L_D2; 1.
DR SMART; SM00878; Biotin_carb_C; 1.
DR SUPFAM; SSF52096; ClpP/crotonase; 2.
DR SUPFAM; SSF56059; Glutathione synthetase ATP-binding domain-like; 1.
DR SUPFAM; SSF52440; PreATP-grasp domain; 1.
DR SUPFAM; SSF51246; Rudiment single hybrid motif; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS50979; BC; 1.
DR PROSITE; PS00188; BIOTIN; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS50989; COA_CT_CTER; 1.
DR PROSITE; PS50980; COA_CT_NTER; 1.
DR PROSITE; PS00867; CPSASE_2; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00409}; Biotin {ECO:0000256|ARBA:ARBA00023267};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00409}.
FT DOMAIN 47..554
FT /note="Biotin carboxylation"
FT /evidence="ECO:0000259|PROSITE:PS50979"
FT DOMAIN 200..394
FT /note="ATP-grasp"
FT /evidence="ECO:0000259|PROSITE:PS50975"
FT DOMAIN 681..755
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 1502..1841
FT /note="CoA carboxyltransferase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50980"
FT DOMAIN 1845..2160
FT /note="CoA carboxyltransferase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS50989"
SQ SEQUENCE 2265 AA; 252532 MW; 5B2925A7EAA66D75 CRC64;
MAAAQRKPSL TSLGRVNGYT NGVLPVRSPA TVSAVDEFCK ALGGKRPIHS ILISNNGMAA
VKFMRSVRTW AYETFGTEKA ILLVAMATPE DMRINAEHIR IADQFVEVPG GTNNNNYANV
QLIVEMAEIT HVDAVWPGWG HASENPELPD ALNAKGIVFL GPPAISMAAL GDKIGSSLIA
QAADVPTLPW SGSHVKMSPD SCLVTIPDEI YREACVYTTD EAIASCQVVG YPAMIKASWG
GGGKGIRKVH NDDEVRALFK QVQGEVPGSP IFIMKVASQS RHLEVQLLCD QYGNVAALHS
RDCSIQRRHQ KIIEEGPITV APIETVKKLE QAARRLAKSV NYVGAATVEY LFSMDTGEYY
FLELNPRLQV EHPVTEWIAE VNLPAAQVAV GMGIPLWQIP EIRRFYGMEH GGNYDAWRRT
SVVATPFDFD KAESTRPKGH CVAVRVTSED PDDGFKPTSG KVQELSFKSK PNVWAYFSVK
SGGGIHEFSD SQFGHVFAFG ESRALAIANM VLGLKEIQIR GEIRTNVDYT IDLLHALDYR
DNKIHTGWLD SRIAMRVRAE RPPWYLSVVG GALYKASASS AAVVSDYIGY LEKGQIPPKH
ISLVNSQVSL NIEGSKYTID MVRGGPGSYR LKMNESEVEA EIHTLRDGGL LMQLDGNSHV
IYAEEEAAGT RLLIDGRTCL LQNDHDPSKL VAETPCKLLR FLISDGSHID GDTPYAEVEV
MKMCMPLLSP ASGIIRFEMS EGQAMQAGEL IARLDLDDPS AVRKAELFHG SFPILGPPTA
VSGKVHQRCA ASLNAAHMIL AGYEHNIDDV VQNLLACLDS PQLPFLQWQE CFSVLATRLP
KDLRSELESK FKEFERISSS QIVDFPAKLL KGILEAHITS CPEKEKASQE RLIEPLMSLV
KSYEGGRESH ARVIVQSLFE EYLSVEELFS DNIRADVIER LRLQYKKDLL KVVDIVLSHQ
GIKSKNKLIM RLMEQLVYPN PAAYRDKLIR FSVLNHINYS ELALKASQLL EQTKLSELRS
NIARSLSELE MFTEDGETMD TPKRKSAINE RMEDLVSAPL AVEDALVGLF DHSDHTLQRR
VVETYVRRLY QPYLVKGSVR MQWHRSGLIA SWEFLEEHIE RKNSAEDETF DKLLVEKHSE
RRWGAMVIIK SLQFLPAIVH AALRETTHNE AVPNGSSEPK CFGNMMHIAL AGINNQMSLL
QDSGDEDQAQ ERINKLAKIL KEQELSSSLR TSGVGVISCI IQRDEGRAPM RHSFHWSAEK
HYYEEEPLLR HLEPPLSIYL ELDKLKGYEN IRYTPSRDRQ WHLYTVKDKP LPIQRMFLRT
LVRQPTTTGS FTSHQGLDIG SSGSLLASYT SRSILRSLVV AMEELELNAH NATGKYDHAH
MYLYVVQEQQ VNDLMPYTKR VEVDAKQEEA AVAQILEELA REIHASVGVK MHRLGVSEWE
VKLWIASSGL ANGAWRFVVT NVTGHTCTTH IYRELEDSST HKVVYHSISV RAPLHGVPVS
AAYKPLGALE RKRLSARNSN TTYCYDFPLA FQTALEMAWA SHSPNDNNIK NKMLLKVTEL
KFSDRSGSWG TPLVPVDPEP GSNDFGMVAW CMEMATPEFP SGRTVLVVSN DVTFKAGSFG
QKEDAFFHAV TDLACAKKLP LIYLAANSGA RLGVAEEVKA RFRVGWSDES NPERGFQYLY
LTPDDYAEMG SSVIAHELKL ANGETRWVID TIIGKEDGLG VENLTGSGAI AGAYSRAYKE
TFTLTYVTGR TVGIGAYLSR LGMRCIQRLD QPIILTGFSA LNKLLGREVY SSHMQLGGPK
IMATNGVVHL TVSDDLEGVS SILKWLSFVP PYVGGPLPIC KPLDPPERPV EYFVENSCDP
RAAIRGVLDS NGNWVGGIFD KDSFIETLEG WARTVVTGRA KLGGIPVGIV AVETQTVMQI
IPADPGQLDS HERVVPQAGQ VWFPDSASKT AQAILDFNRE ELPLFILANW RGFSGGQRDL
FEGILQAGST IVENLRTYKQ PVFVYIPMMG ELRGGAWVVV DSQINPDHIE MYAERTAKGN
ILEPEGMIEI KFKTKDLIEC MGRLDEELVG LKSKLEESRR AGALEVVNSL QQKIKAREKS
LLPVYTQMAT KFAELHDTSL RMAVKGVIRE VVDWKTSRSY FYRRLHRRIA EASLIKTVED
ARGNQLSRTS AYAFMKSWFL NSDVAKGRED AWNDDELFFT WKDDTRNYED KLKELRIQKV
LQQLTDVGSS ELDLRALPQG LLALLDKMEP SSRASLVQKL LEVLS
//
