ID A0A060UU13_9PROT Unreviewed; 587 AA.
AC A0A060UU13;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=Nif-specific regulatory protein {ECO:0000256|ARBA:ARBA00015308, ECO:0000256|RuleBase:RU368029};
GN Name=nifA {ECO:0000313|EMBL:CDQ10288.1};
GN ORFNames=AFERRI_400069 {ECO:0000313|EMBL:CDQ10288.1};
OS Acidithiobacillus ferrivorans.
OC Bacteria; Pseudomonadota; Acidithiobacillia; Acidithiobacillales;
OC Acidithiobacillaceae; Acidithiobacillus.
OX NCBI_TaxID=160808 {ECO:0000313|EMBL:CDQ10288.1, ECO:0000313|Proteomes:UP000029069};
RN [1] {ECO:0000313|EMBL:CDQ10288.1, ECO:0000313|Proteomes:UP000029069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF27 {ECO:0000313|EMBL:CDQ10288.1,
RC ECO:0000313|Proteomes:UP000029069};
RA Talla E., Hedrich S., Mangenot S., Ji B., Johnson D.B., Barbe V.,
RA Bonnefoy V.;
RT "Initial genome analysis of the psychrotolerant acidophile
RT Acidithiobacillus ferrivorans CF27: insights into iron and sulfur oxidation
RT pathways and into biofilm formation.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for activation of most nif operons, which are
CC directly involved in nitrogen fixation. {ECO:0000256|ARBA:ARBA00002167,
CC ECO:0000256|RuleBase:RU368029}.
CC -!- SUBUNIT: Interacts with sigma-54. {ECO:0000256|ARBA:ARBA00011135,
CC ECO:0000256|RuleBase:RU368029}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDQ10288.1}.
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DR EMBL; CCCS020000035; CDQ10288.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A060UU13; -.
DR Proteomes; UP000029069; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-UniRule.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.8.60; -; 1.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR010113; Nif-specific_regulatory_prot.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR NCBIfam; TIGR01817; nifA; 1.
DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1.
DR PANTHER; PTHR32071:SF95; TRANSCRIPTIONAL REGULATORY PROTEIN ZRAR; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR PRINTS; PR01590; HTHFIS.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 4: Predicted;
KW Activator {ECO:0000256|RuleBase:RU368029};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU368029};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|RuleBase:RU368029};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Transcription {ECO:0000256|RuleBase:RU368029};
KW Transcription regulation {ECO:0000256|RuleBase:RU368029};
KW Two-component regulatory system {ECO:0000256|RuleBase:RU368029}.
FT DOMAIN 243..471
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000259|PROSITE:PS50045"
FT REGION 13..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 587 AA; 65268 MW; 4A3AB40DC3024559 CRC64;
MPTIVGVSCL AQASGRRTGS AKPQPPHPTP TPPERCREEG LIMKDHVTPN HFQELTTIYE
VSKVLSSSMD LEHTLNDVLK TLESHLHISP SMIVLRMNEE IVGVIAATGL SVAEIERGRY
RLGEGMIGKI MRAGMPIVIP DVSQEPAFLH RAFEPEISQR LHAFVGVPIK VGYECIGVLA
IYRSERHKGV SFRSDVRLLQ MIANLVGQTV NLHQGISAER NRLLQEKHRL QKELQKRYSI
KNVIGNSRAM QAVFSDVHQA APGKATVLLR GESGTGKEVI ARAIHYLSPR RDKAFVSLNC
AALPESILES ELFGHERGAF TGATQERKGR FELADGGTLF LDEIGDISPA FQAKLLRVLQ
EREFERVGGS QTRRVNVRII TATNRNLEEA VSKGEFRADL YYRINVVSIF LPPLRERRED
IPALIEHFLA RHNADNQRKL TITPEALQTM VSCYWPGNVR ELENCVERTA TMMRGDIIRN
LDVPCQRNQC LSMALRPLEG SHPIVTMKVS GGGSIPLPVM PEAAEQVVDA VAASDMEVSP
PLSERDRLIQ AMDRCGWVQA KAARLLGTTT RKLGYALNKH GVEVKKL
//
