ID   A0A061GQG0_THECC        Unreviewed;       795 AA.
AC   A0A061GQG0;
DT   03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT   03-SEP-2014, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   SubName: Full=Isoamylase 1 isoform 2 {ECO:0000313|EMBL:EOY32085.1};
GN   ORFNames=TCM_039557 {ECO:0000313|EMBL:EOY32085.1};
OS   Theobroma cacao (Cacao) (Cocoa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Byttnerioideae; Theobroma.
OX   NCBI_TaxID=3641 {ECO:0000313|EMBL:EOY32085.1, ECO:0000313|Proteomes:UP000026915};
RN   [1] {ECO:0000313|EMBL:EOY32085.1, ECO:0000313|Proteomes:UP000026915}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Matina 1-6 {ECO:0000313|Proteomes:UP000026915};
RX   PubMed=23731509; DOI=10.1186/gb-2013-14-6-r53;
RA   Motamayor J.C., Mockaitis K., Schmutz J., Haiminen N., Iii D.L.,
RA   Cornejo O., Findley S.D., Zheng P., Utro F., Royaert S., Saski C.,
RA   Jenkins J., Podicheti R., Zhao M., Scheffler B.E., Stack J.C., Feltus F.A.,
RA   Mustiga G.M., Amores F., Phillips W., Marelli J.P., May G.D., Shapiro H.,
RA   Ma J., Bustamante C.D., Schnell R.J., Main D., Gilbert D., Parida L.,
RA   Kuhn D.N.;
RT   "The genome sequence of the most widely cultivated cacao type and its use
RT   to identify candidate genes regulating pod color.";
RL   Genome Biol. 14:R53.1-R53.24(2013).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000256|ARBA:ARBA00004229}.
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DR   EMBL; CM001887; EOY32085.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A061GQG0; -.
DR   EnsemblPlants; EOY32085; EOY32085; TCM_039557.
DR   Gramene; EOY32085; EOY32085; TCM_039557.
DR   HOGENOM; CLU_011725_1_1_1; -.
DR   Proteomes; UP000026915; Chromosome 9.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048650; ISOA1-3-like_C.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21156; ISOA1-3_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW   Plastid {ECO:0000256|ARBA:ARBA00022640};
KW   Reference proteome {ECO:0000313|Proteomes:UP000026915};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   DOMAIN          233..668
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   795 AA;  90081 MW;  C8521F836CBB34C3 CRC64;
     MDLTCCTFCL QYAPKLIPAT LRRQTPPKPN LRTVSNNHAT TSTTRMVVKA RGGGGGGARR
     GAAEADTAVV EKPKIKRFQL SEGHPGPFGA TLLDGGVNFA IYSANAVSAT LCLITFSDLQ
     QNRVTEQISL DPLTNKTGDV WHVFLRGDFK DMLYGYIFDG KFSPEKGHYY DSSKILLDPY
     AKAVISRGEF GALGPEDNCW PQMACMVPTS EDEFDWEGDL PLRYSQRDLI IYEMHVRGFT
     RHESSRTEFP GTYRGVVEKL DHLKELGVNC IELMPCHEFN ELEYYSYNSI LGDYKLNFWG
     YSTVNFFSPM IRYSSSGMHN CGRDAVNEFK YLVKEAHKRG IEVFMDVVFN HTAEGNENGP
     ILSFRGVDNS VYYMLAPKGE YYNYSGCGNT FNCNHPVVRQ FILDCLRYWV TEMHVDGFRF
     DLASIMTRSS SLWDLVNVFG NPLKGDLITT GTPLSSPPLI DMMSNDPVLR GVKLIAEAWD
     TGGLYQVGSF PHWGIWSEWN GKYRDIVRQF IKGTDGFSGA FAECLCGSPN LYQEGGRKPW
     NSINFVCAHD GFTLADLVTY NNKHNLANGE DNNDGESHNN SWNCGQEGEF ASISVKKLRK
     RQMRNFFLCL MVSQGIPMIC MGDEYGHTKG GNNNTYCHDN YINYFQWDKK EECSSDFFRF
     CCLMTKFRRE CESLGLNDFP TAERLQWHGH TPGMPDWSDR SRFVAFTLID SVKGEIYVAF
     NTSYLPVTIT LPEHPGYRWE PLVDTSKPAP FDFLSEELKE RDIAIKQYVQ FLDANLYPML
     SYSSIILLLS PDGNA
//