ID A0A061HLM5_BLUGR Unreviewed; 410 AA.
AC A0A061HLM5;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Glycosidase {ECO:0000256|PIRNR:PIRNR037299};
DE EC=3.2.-.- {ECO:0000256|PIRNR:PIRNR037299};
GN ORFNames=BGT96224_1257 {ECO:0000313|EMBL:EPQ67661.1},
GN BGT96224V2_LOCUS46 {ECO:0000313|EMBL:SUZ07101.1};
OS Blumeria graminis f. sp. tritici 96224.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Erysiphales; Erysiphaceae; Blumeria.
OX NCBI_TaxID=1268274 {ECO:0000313|EMBL:SUZ07101.1};
RN [1] {ECO:0000313|Proteomes:UP000053110}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=96224 {ECO:0000313|Proteomes:UP000053110};
RX PubMed=23852167; DOI=10.1038/ng.2704;
RA Wicker T., Oberhaensli S., Parlange F., Buchmann J.P., Shatalina M.,
RA Roffler S., Ben-David R., Dolezel J., Simkova H., Schulze-Lefert P.,
RA Spanu P.D., Bruggmann R., Amselem J., Quesneville H.,
RA Ver Loren van Themaat E., Paape T., Shimizu K.K., Keller B.;
RT "The wheat powdery mildew genome shows the unique evolution of an obligate
RT biotroph.";
RL Nat. Genet. 45:1092-1096(2013).
RN [2] {ECO:0000313|EMBL:EPQ67661.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=96224 {ECO:0000313|EMBL:EPQ67661.1};
RA Oberhaensli S., Wicker T., Keller B.;
RT "The wheat powdery mildew genome reveals unique evolution of an obligate
RT biotroph.";
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SUZ07101.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=96224 {ECO:0000313|EMBL:SUZ07101.1};
RA Quirk P.G., Krulwich T.A.;
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family.
CC {ECO:0000256|PIRNR:PIRNR037299}.
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DR EMBL; KE373420; EPQ67661.1; -; Genomic_DNA.
DR EMBL; UIGY01000001; SUZ07101.1; -; Genomic_DNA.
DR HOGENOM; CLU_027506_2_0_1; -.
DR OrthoDB; 337487at2759; -.
DR Proteomes; UP000053110; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:InterPro.
DR CDD; cd02183; GH16_fungal_CRH1_transglycosylase; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR000757; GH16.
DR InterPro; IPR017168; Glyco_hydro_16_CRH1_prd.
DR PANTHER; PTHR10963:SF27; GLYCOSIDASE CRH1-RELATED; 1.
DR PANTHER; PTHR10963; GLYCOSYL HYDROLASE-RELATED; 1.
DR Pfam; PF00722; Glyco_hydro_16; 1.
DR PIRSF; PIRSF037299; Glycosidase_CRH1_prd; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS51762; GH16_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR037299-2};
KW Glycosidase {ECO:0000313|EMBL:EPQ67661.1};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR037299};
KW Membrane {ECO:0000256|PIRNR:PIRNR037299};
KW Reference proteome {ECO:0000313|Proteomes:UP000053110};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..410
FT /note="Glycosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5040560134"
FT DOMAIN 1..244
FT /note="GH16"
FT /evidence="ECO:0000259|PROSITE:PS51762"
FT REGION 274..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 310..361
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..387
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 121
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR037299-1"
FT ACT_SITE 125
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR037299-1"
FT DISULFID 27..34
FT /evidence="ECO:0000256|PIRSR:PIRSR037299-2"
SQ SEQUENCE 410 AA; 42892 MW; E78CAB85AEED1E70 CRC64;
MRFSDISVPA TVFLILNQAY AQTSTDCNPL QKTCPPNPGL GTSVFTDFTK GKSDYWQPLQ
GTTMKYDGPN GAQFTIQKSS DAPTMSLTKF IMFGHIDMIA KASPGTGIVS SFVLLSKDLD
EIDWEWLGGI NSNVQTNFFG KGDVSSYDRG TTVAVQTPVD SFHTYSLDWT AERIVWTIDG
QTVRTLAYAD AVAKSGKNYP QTPMEVKMGS WIGCLDYSNP ETKGTCEWAG GQADLTKAPF
TMSVKSVNVT DYGCGGEYTY SDNSGSWQSI KSSGACDGKG TGPGAPNSAE APDDSIVPIA
GAVAPVNPNK ASGPSGSLTP DDGPAASPNT KGTLSKGTPS NGTSSNGTTP NSTNTPGTAI
STGVAKPPPA VSSGVPNINT SDANKKSKRS YGPMEFTVIA LGLGLGYFVM
//
