ID A0A067CRS0_SAPPC Unreviewed; 1610 AA.
AC A0A067CRS0;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=EF-hand domain-containing protein {ECO:0000259|PROSITE:PS50222};
GN ORFNames=SPRG_02221 {ECO:0000313|EMBL:KDO33414.1};
OS Saprolegnia parasitica (strain CBS 223.65).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae;
OC Saprolegnia.
OX NCBI_TaxID=695850 {ECO:0000313|EMBL:KDO33414.1, ECO:0000313|Proteomes:UP000030745};
RN [1] {ECO:0000313|EMBL:KDO33414.1, ECO:0000313|Proteomes:UP000030745}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 223.65 {ECO:0000313|EMBL:KDO33414.1,
RC ECO:0000313|Proteomes:UP000030745};
RX PubMed=23785293;
RA Jiang R.H., de Bruijn I., Haas B.J., Belmonte R., Lobach L., Christie J.,
RA van den Ackerveken G., Bottin A., Bulone V., Diaz-Moreno S.M., Dumas B.,
RA Fan L., Gaulin E., Govers F., Grenville-Briggs L.J., Horner N.R.,
RA Levin J.Z., Mammella M., Meijer H.J., Morris P., Nusbaum C., Oome S.,
RA Phillips A.J., van Rooyen D., Rzeszutek E., Saraiva M., Secombes C.J.,
RA Seidl M.F., Snel B., Stassen J.H., Sykes S., Tripathy S., van den Berg H.,
RA Vega-Arreguin J.C., Wawra S., Young S.K., Zeng Q., Dieguez-Uribeondo J.,
RA Russ C., Tyler B.M., van West P.;
RT "Distinctive expansion of potential virulence genes in the genome of the
RT oomycete fish pathogen Saprolegnia parasitica.";
RL PLoS Genet. 9:E1003272-E1003272(2013).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|RuleBase:RU003808}; Multi-
CC pass membrane protein {ECO:0000256|RuleBase:RU003808}.
CC -!- SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC
CC 1.A.1.11) family. {ECO:0000256|RuleBase:RU003808}.
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DR EMBL; KK583193; KDO33414.1; -; Genomic_DNA.
DR RefSeq; XP_012196160.1; XM_012340770.1.
DR STRING; 695850.A0A067CRS0; -.
DR EnsemblProtists; KDO33414; KDO33414; SPRG_02221.
DR GeneID; 24124782; -.
DR KEGG; spar:SPRG_02221; -.
DR VEuPathDB; FungiDB:SPRG_02221; -.
DR OMA; VCTLNQT; -.
DR OrthoDB; 297674at2759; -.
DR Proteomes; UP000030745; Unassembled WGS sequence.
DR GO; GO:0005891; C:voltage-gated calcium channel complex; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005245; F:voltage-gated calcium channel activity; IEA:InterPro.
DR Gene3D; 1.10.287.70; -; 4.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR Gene3D; 1.20.120.350; Voltage-gated potassium channels. Chain C; 4.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR031649; GPHH_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR002077; VDCCAlpha1.
DR InterPro; IPR043203; VGCC_Ca_Na.
DR InterPro; IPR027359; Volt_channel_dom_sf.
DR PANTHER; PTHR10037:SF62; SODIUM CHANNEL PROTEIN 60E; 1.
DR PANTHER; PTHR10037; VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM; 1.
DR Pfam; PF16905; GPHH; 1.
DR Pfam; PF00520; Ion_trans; 4.
DR PRINTS; PR00167; CACHANNEL.
DR SMART; SM00054; EFh; 1.
DR SUPFAM; SSF81324; Voltage-gated potassium channels; 4.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR602077-1};
KW Calcium channel {ECO:0000256|ARBA:ARBA00022673,
KW ECO:0000256|RuleBase:RU003808};
KW Calcium transport {ECO:0000256|ARBA:ARBA00022568,
KW ECO:0000256|RuleBase:RU003808};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR602077-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000030745};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW Voltage-gated channel {ECO:0000256|ARBA:ARBA00022882,
KW ECO:0000256|RuleBase:RU003808}.
FT TRANSMEM 66..90
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 148..166
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 202..225
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 383..405
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 465..484
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 504..524
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 583..609
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 672..695
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 786..803
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 815..840
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 852..874
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 913..931
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1076..1097
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1156..1174
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1186..1208
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1220..1245
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1266..1291
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1420..1455
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT BINDING 1047
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR602077-1"
SQ SEQUENCE 1610 AA; 180580 MW; 315CCB8B44FF2F88 CRC64;
MRKQPSSTKV YPTAPTRAEL TPAEADLPRK SSLAPPLGNR WVRRAVLGNA GDQAHLATLR
GLATRLLAST WFATFITLVV LLNTITLGLV DYSDPWADGP NPTLLRNRLV DQMNNISLGI
FLAEAVLKVL SMGFVYGPTA YCKDYWNLLD LVVLLSGCLG WFFHAGSVGQ LRILRVLRPL
RTLHSFPGLK VLVNGVLSSL PALFDVGILL GFSYLVFAIL GMEIWSGTYH QRCRLTEFPV
ILNFDAANAP SAAAYPNKTY LDLVLANPSA YRCPRTDVEN DNWTEPLNCF WPLNANDIQY
CGSRPCAPGT FCGSNFDASG RPRFRNVLLN GSMLVDMATE GDFLPNLNFG LTSFDTVGHT
LVIIAQIVTA SGWMVLTQNT QDAYSWLAAG VYYNTVLFLG MCFLLQLNMA VMFNEYEKAK
TALDKATARP LGLIRRRLQR QYYRRVKTLP LSTLRRHVRH VVTSVRFHRL GLGMTLLNVA
LLASDHHPQT ASFRANVEIG NFCFLLYFTM EMALKIYGLG FTVYRQDRFN LYDMVTILMG
GVETLLHPPA FLDGTPGARS PFAILRAARA IKLAYSWPSF QRLLTAIVGA LQEILNFLFF
LVLFIYMYAL MGMELFATKY KFDALNRPQP FNATDPSVVL HRSNFDTIQW AFFTVFQVIT
YDNWPMVMYD GWLSAGLVAP FYFVSIVILG VWVVMNMFSA IMVQSVMEDA SAKPAIVGDV
DANLRTRLRL RQLRRILRRL MGVHSVLSAP TLDTDDSSLG RKSFGCFGLS SPLRQVSLAL
VRHPRWHVFV TMSIVVSCIV TALDTPLLNP HAGLGYAMTI INNALAIVFT LEMLLTMLAL
GFTAYLTDPW RVLDLFIVGV CLLSWSATGV FAQLRALRSL RALRPLRVIH RLPQLQVVVN
TLFRCLPDIG RSLLFFVFML LLFGIACVKF FKGSMSTCSV SPYNYLDTPL YTPPPPWFPA
SYAGAYSMAD LERLDVMSFP RPWSNLTVAS QMALAPYWNR TCHFGERLPT SKDVCECFNM
TWQPVVPQRF DNIALALGSL YELTTMEGWS FVAIAAVDAT GPDSQPLRNN QEGWMVFWFL
FMIVGAFFVT NLFIGVLCDS FMRENYGVMV TDEQLDWIKL QRKVLTMAPV VRYPPPSEST
WRHACYKLVE RPAFETSVMA AILINLFVMS TGSFGQPSWV RTTSTVATNL LSAAFALEAA
LKIAAFGPRY FASSAHRFDL AVVLLALIGM GLPDSVGMGT IATVVRVFRV GRVLRLFQKA
TLLKSLYDTI MVSLPAMGNV TALLMLLYYI FAAVGVQLFA KVAYGPSMVN PHQNFQNFWL
ALQTLIGFST GENWNNFLWE LYHISPTSNP ECMEPAFNAS VCGFGDALDC VPLDGCGSWY
IVPFMYLFEL VVGYIGLNLF SGIVVDAIGD SCAKSALSPS NLAEFTAMWA EYDPNGSGFV
TIDDLCRLLS RLNAPFGFKL VAHMTTRKIY HAIGGLDIPI YDGRLVHFKD VPRALVQRSL
SEGDQNKFYE IGALMARLGI AKQFDDVWQR SHSKAMVRDL RARAVTPIRD FVAFLIVAKF
LRCVRYRLER RRRLYRRLLH TELQSRRERI EAKLHPHHLP PLERVPPALL
//
