ID A0A067EHL2_CITSI Unreviewed; 896 AA.
AC A0A067EHL2;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Aconitate hydratase {ECO:0000256|ARBA:ARBA00012926, ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926, ECO:0000256|RuleBase:RU361275};
GN ORFNames=CISIN_1g001917mg {ECO:0000313|EMBL:KDO54659.1};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO54659.1, ECO:0000313|Proteomes:UP000027120};
RN [1] {ECO:0000313|EMBL:KDO54659.1, ECO:0000313|Proteomes:UP000027120}
RP NUCLEOTIDE SEQUENCE.
RG International Citrus Genome Consortium;
RA Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
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DR EMBL; KK784993; KDO54659.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067EHL2; -.
DR Proteomes; UP000027120; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043436; P:oxoacid metabolic process; IEA:UniProt.
DR CDD; cd01586; AcnA_IRP; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000027120}.
FT DOMAIN 163..666
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 795..891
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 896 AA; 97759 MW; 6379AD50B9BECD42 CRC64;
MYITTSTSAA SSILRASSRS RFVSSLSSFK SLPARSLSPS PSSLVSQRSL GFASAVRSFR
CSVPRWSHRV DWRSPLSLRA QIRTVAPAIE RLERAFATMA AEHPFKEILT ALPKPGGGEF
GKFYSLPALN DPRIEKLPYS IRILLESAIR NCDNFQVKKE DVEKIIDWEN SAPKQVEIPF
KPARVLLQDF TGVPAVVDLA CMRDAMNKLG SDSNKINPLV PVDLVIDHSV QVDVTRSENA
VKANMELEFQ RNKERFAFLK WGSSAFHNML VVPPGSGIVH QVNLEYLGRV VFNTNGMLYP
DSVVGTDSHT TMIDGLGVAG WGVGGIEAEA AMLGQPMSMV LPGVVGFKLS GKLHNGVTAT
DLVLTVTQML RKHGVVGKFV EFHGDGMGEL SLADRATIAN MSPEYGATMG FFPVDHVTLQ
YLKLTGRSDE TVAMVEGYLR ANKMFVDYNE PQQERVYSSY LELNLADVEP CISGPKRPHD
RVPLKEMKAD WHSCLDNKVG FKGFAVPKET QEKVVKFSFH GQPAELKHGS VVIAAITSCT
NTSNPSVMLG AGLVAKKACE LGLQVKPWVK TSLAPGSGVV TKYLLQSGLQ KYLNEQGFHI
VGYGCTTCIG NSGDLDESVA STITDNDIVA AAVLSGNRNF EGRVHPLTRA NYLASPPLVV
AYALAGTVDI DFDKEPIGTT KDGKSVYFKD IWPTTEEIAE VVQSSVLPDM FKSTYEAITK
GNPTWNQLSV PASKLYSWDP NSTYIHEPPY FKDMTMDPPG AHGVKDAYCL LNFGDSITTD
HISPAGSIHK DSPTAKYLLE RGVERRDFNS YGSRRGNDEV MARGTFANIR LVNKLLNGEV
GPKTVHVPTG EKLSVFDAAM KYKSAGHGTI ILAGAEYGSG SSRDWAAKGP MLELKQ
//
