ID A0A067FVH3_CITSI Unreviewed; 898 AA.
AC A0A067FVH3;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=CISIN_1g002610mg {ECO:0000313|EMBL:KDO71343.1};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711 {ECO:0000313|EMBL:KDO71343.1, ECO:0000313|Proteomes:UP000027120};
RN [1] {ECO:0000313|EMBL:KDO71343.1, ECO:0000313|Proteomes:UP000027120}
RP NUCLEOTIDE SEQUENCE.
RG International Citrus Genome Consortium;
RA Gmitter F., Chen C., Farmerie W., Harkins T., Desany B., Mohiuddin M.,
RA Kodira C., Borodovsky M., Lomsadze A., Burns P., Jenkins J., Prochnik S.,
RA Shu S., Chapman J., Pitluck S., Schmutz J., Rokhsar D.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
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DR EMBL; KK784889; KDO71343.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067FVH3; -.
DR Proteomes; UP000027120; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043436; P:oxoacid metabolic process; IEA:UniProt.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF62; ACONITATE HYDRATASE 1; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000027120}.
FT DOMAIN 65..566
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 695..822
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 898 AA; 98347 MW; 053D294E83550E67 CRC64;
MATENPFKSI LKTLQRPDGG EFGKYYSLPA LNDPRIDKLP YSIKILLESA IRNCDEFQVK
SKDVEKIIDW ETTSPKQVEI PFKPARVLLQ DFTGVPAVVD LACMRDAMNK LGGDSNKINP
LVPVDLVIDH SVQVDVARSE NAVQANMEFE FRRNKERFAF LKWGSNAFHN MLVVPPGSGI
VHQVNLEYLG RVVFNTNGML YPDSVVGTDS HTTMIDGLGV AGWGVGGIEA EAAMLGQPMS
MVLPGVVGFK LSGKLRDGVT ATDLVLTVTQ MLRKHGVVGM FVEFYGEGMS ELSLADRATI
ANMSPEYGAT MGFFPVDHVT LQYLKLTGRS DDTVSMIESY LRANKMFVDY SESERVYSSY
LELNLEEVVP CVSGPKRPHD RVPLNEMKAD WHACLDNRVG FKGFAIPKEY QSKVAEFNFH
GTPAQLRHGD VVIAAITSCT NTSNPSVMLG AALVAKKACE LGLEVKPWIK TSLAPGSGVV
TKYLQNSGLQ KYLNHLGFHI VGYGCTTCIG NSGDIDDAVA AAITENDIVA AAVLSGNRNF
EGRVHPLTRA NYLASPPLVV AYALAGSVNI DFETEPVGVG KDGKKIFLRD IWPSSEEVAH
VVQKSVLPDM FKATYEAITK GNPMWNQLSV PSGTLYAWDP KSTYIHEPPY FKDMTMSPPG
PHGVKGAYCL LNFGDSITTD HISPAGSIHK DSPAAKYLME RGVDRRDFNS YGSRRGNDEI
MARGTFANIR LVNKLLNGEV GPKTIHIPTG EKLSVFDAAM RYKNEGHDTV ILAGAEYGSG
SSRDWAAKGP MLLGVKAVIA KSFERIHRSN LVGMGIIPLC FKPGEDAETH GLTGHERYTI
DLPSSVSEIR PGQDVRVVTD SGKSFTCVIR FDTEVELAYF DHGGILQYVI RNLINVRQ
//
