ID A0A067NE63_PLEOS Unreviewed; 907 AA.
AC A0A067NE63;
DT 03-SEP-2014, integrated into UniProtKB/TrEMBL.
DT 03-SEP-2014, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=PLEOSDRAFT_1044381 {ECO:0000313|EMBL:KDQ26149.1};
OS Pleurotus ostreatus PC15.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX NCBI_TaxID=1137138 {ECO:0000313|EMBL:KDQ26149.1, ECO:0000313|Proteomes:UP000027073};
RN [1] {ECO:0000313|Proteomes:UP000027073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PC15 {ECO:0000313|Proteomes:UP000027073};
RX PubMed=24958869; DOI=10.1073/pnas.1400592111;
RA Riley R., Salamov A.A., Brown D.W., Nagy L.G., Floudas D., Held B.W.,
RA Levasseur A., Lombard V., Morin E., Otillar R., Lindquist E.A., Sun H.,
RA LaButti K.M., Schmutz J., Jabbour D., Luo H., Baker S.E., Pisabarro A.G.,
RA Walton J.D., Blanchette R.A., Henrissat B., Martin F., Cullen D.,
RA Hibbett D.S., Grigoriev I.V.;
RT "Extensive sampling of basidiomycete genomes demonstrates inadequacy of the
RT white-rot/brown-rot paradigm for wood decay fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:9923-9928(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KL198010; KDQ26149.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A067NE63; -.
DR STRING; 1137138.A0A067NE63; -.
DR VEuPathDB; FungiDB:PLEOSDRAFT_1044381; -.
DR HOGENOM; CLU_005015_1_1_1; -.
DR InParanoid; A0A067NE63; -.
DR OrthoDB; 2504097at2759; -.
DR Proteomes; UP000027073; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KDQ26149.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000027073};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 241..338
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 714..818
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 822..886
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 907 AA; 103760 MW; 49B6D1698BE9E21E CRC64;
MLNTVLDKGW FWKQRQDSIA EVVKELAHGL PDDSHDVSRG SIIGGWQLAV AFPSEVHVEL
LRSQWIPHPF VAFNEHKVQW IGEVEWLYYC TFKGPRLEPG QCSELIFEGL DTVCDVYLNQ
SKIISADNMF RTYTYLLDGE GPATSSQQNT LLLHFKPAKK LAKAEEEKYG KVRAGSTNLG
DPSRVYLRKA QYDWRWDWGP ELMTCGPYRS IRIETYLARI QEVQPCITVH FPESSSSTFV
ARVSVNAVVQ GDFNKDYAIV VSLKHLNGRT IRTARQEIPH KTEKLKKVSL KGIVDWSLND
DNIRLWWPVG YGTQSLYSLQ VDLTDEVIHQ ITKRIGFRSV SLVQEPLKEP DQYGTGSTFL
FEINRVRMFM GGSNWVPADN FLTTITPERY RAWLTLLVKG NQNMVRLWGG GVYEPDVFYD
ICDELGILVW QDFQFACGVY PAHDEFVENV RKEAEDNVRR LRHHPSIALF CGNNEDYQQV
LQWGGIDTLP ARKIYEEVLP AVVSGLTEPP IPYHRGSPYG GKGWDTSDPT IGDVHQWNIW
GGKELPYQSY DELGGRFVSE FGIPSMPNMK TIESWMEGAA ESDWHPQSIW MAQHTRAGSF
ERRFAILMNE NFRMTSDFET FATSYAYQSW RRKWRGKGKE YTAGALIWQL NDCWPVTSWA
IVDYYLRPKP AYYTISRQLA EVAVGITRTV IQNRDHDRPR QFYEFGAIQS HGARIDIWGM
NSSLQLIDAE LELYCVDLNS DWFHKETRGV HLLPNQSTEL ISMPCPGPPK ASARKDADPY
KVPEWTSTSS VIVSARLINP STGEVLARHA DWPQPFKYLR IPDPKITVKV DAVKEELRVS
VDRPAKGVFF NIDGGGTESQ EFGDGILWSD NALDMMPGEN RIINVSGLRN RRIYVTYLGK
EKPSLVQ
//
