ID   A0A074WDE5_9PEZI        Unreviewed;       765 AA.
AC   A0A074WDE5;
DT   01-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2014, sequence version 1.
DT   27-MAR-2024, entry version 50.
DE   RecName: Full=asparagine--tRNA ligase {ECO:0000256|ARBA:ARBA00012816};
DE            EC=6.1.1.22 {ECO:0000256|ARBA:ARBA00012816};
GN   ORFNames=M436DRAFT_85430 {ECO:0000313|EMBL:KEQ69559.1};
OS   Aureobasidium namibiae CBS 147.97.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Dothideales; Saccotheciaceae; Aureobasidium.
OX   NCBI_TaxID=1043004 {ECO:0000313|EMBL:KEQ69559.1, ECO:0000313|Proteomes:UP000027730};
RN   [1] {ECO:0000313|EMBL:KEQ69559.1, ECO:0000313|Proteomes:UP000027730}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 147.97 {ECO:0000313|EMBL:KEQ69559.1,
RC   ECO:0000313|Proteomes:UP000027730};
RX   PubMed=24984952;
RA   Gostin Ar C., Ohm R.A., Kogej T., Sonjak S., Turk M., Zajc J., Zalar P.,
RA   Grube M., Sun H., Han J., Sharma A., Chiniquy J., Ngan C.Y., Lipzen A.,
RA   Barry K., Grigoriev I.V., Gunde-Cimerman N.;
RT   "Genome sequencing of four Aureobasidium pullulans varieties:
RT   biotechnological potential, stress tolerance, and description of new
RT   species.";
RL   BMC Genomics 15:549-549(2014).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000256|ARBA:ARBA00006375}.
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DR   EMBL; KL584721; KEQ69559.1; -; Genomic_DNA.
DR   RefSeq; XP_013423786.1; XM_013568332.1.
DR   AlphaFoldDB; A0A074WDE5; -.
DR   STRING; 1043004.A0A074WDE5; -.
DR   GeneID; 25417532; -.
DR   HOGENOM; CLU_011004_1_0_1; -.
DR   OrthoDB; 5481380at2759; -.
DR   Proteomes; UP000027730; Unassembled WGS sequence.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro.
DR   GO; GO:0048250; P:iron import into the mitochondrion; IEA:UniProt.
DR   CDD; cd04318; EcAsnRS_like_N; 1.
DR   Gene3D; 1.50.40.10; Mitochondrial carrier domain; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004522; Asn-tRNA-ligase.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   NCBIfam; TIGR00457; asnS; 1.
DR   PANTHER; PTHR22594:SF34; ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED; 1.
DR   PANTHER; PTHR22594; ASPARTYL/LYSYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF00153; Mito_carr; 3.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR   SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000313|EMBL:KEQ69559.1}; ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW   ProRule:PRU00282}; Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW   Mitochondrion inner membrane {ECO:0000256|ARBA:ARBA00022792};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW   Reference proteome {ECO:0000313|Proteomes:UP000027730};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW   ProRule:PRU00282}; Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT   DOMAIN          159..501
FT                   /note="Aminoacyl-transfer RNA synthetases class-II family
FT                   profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50862"
FT   REPEAT          479..567
FT                   /note="Solcar"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
FT   REPEAT          575..659
FT                   /note="Solcar"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
FT   REPEAT          666..757
FT                   /note="Solcar"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00282"
SQ   SEQUENCE   765 AA;  83592 MW;  8AAD2AFCBED37273 CRC64;
     MKTWRGLSHR TLRTNASLRW SSTIQKPANI ASLLKSSAET TDESTPEIEI NGYVRTIRKQ
     KRIAFAAVGD GSSMQPVQAV LTPDQALSLS TGDAIRLRGN WQKCPPGKAQ THELQVSEAT
     KLGYNDATTN PIQKKYQSPE FLRTLPHLRS RLPFNALVLH LRSQVIGSIT NFFIKEGFVQ
     THPPILTSSD CEGAGEVFTV SSNASKDSIQ KSEDGEVEHF FRAPKYLTVS SQLHLEALAQ
     SVGNVWTLSP TFRAEKSDTP RHLSEFYMLE AEMCFVDDAN QVMNLIENML STMASDLQQS
     HTVQQILQAK ASQTSEEAAE EGAVTADQLS RRWQGMAQSN WPRVSYAKAI ETLEEAMKQG
     TVFQFTPSWE EGLQAEHERF LAEHFGKGQP VFIADYPIEQ KPFYMSKTPG SDRTVACFDL
     LVPDLCELIG GSMREQNLPA IQKAMQARGM LRELTPEQRA AGESEIPEYK LYEGLPPNFS
     LAANMAAGAF AGVAEHSVMY PIDLLKTRMQ VMSPTPAAIY TGLGNAIATI SRAEGYLSLW
     RGLSSVIVGA GPAHAVYFAT YEVVKQQMGG NAAGHHPLAA ATSGACATIA SDAFMNPFDV
     IKQRMQVHGS VYTSITQCAR SVFRNEGLRA FYVSYPTTLA MTVPFTALQF TAYESITKAM
     HRRDGYDPLT HCTAGGLAGG IAAAATTPLD VIKTLLQTRG SSTDAEIRGA KGLFDAAGII
     ARRDGAKGFF RGMKARVVTA APSTAICWSA YEVAKAYFIR AEEAK
//