ID A0A077MCE4_9MICO Unreviewed; 1145 AA.
AC A0A077MCE4;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BN13_910002 {ECO:0000313|EMBL:CCI54971.1};
OS Tetrasphaera jenkinsii Ben 74.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Tetrasphaera.
OX NCBI_TaxID=1193518 {ECO:0000313|EMBL:CCI54971.1, ECO:0000313|Proteomes:UP000035720};
RN [1] {ECO:0000313|EMBL:CCI54971.1, ECO:0000313|Proteomes:UP000035720}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ben 74 {ECO:0000313|EMBL:CCI54971.1,
RC ECO:0000313|Proteomes:UP000035720};
RX PubMed=23178666; DOI=10.1038/ismej.2012.136;
RA Kristiansen R., Nguyen H.T.T., Saunders A.M., Nielsen J.L., Wimmer R.,
RA Le V.Q., McIlroy S.J., Petrovski S., Seviour R.J., Calteau A.,
RA Nielsen K.L., Nielsen P.H.;
RT "A metabolic model for members of the genus Tetrasphaera involved in
RT enhanced biological phosphorus removal.";
RL ISME J. 7:543-554(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCI54971.1}.
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DR EMBL; CAJC01000207; CCI54971.1; -; Genomic_DNA.
DR RefSeq; WP_048547672.1; NZ_HF571038.1.
DR AlphaFoldDB; A0A077MCE4; -.
DR STRING; 1193518.BN13_910002; -.
DR Proteomes; UP000035720; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000035720}.
FT DOMAIN 15..387
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 388..728
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 300..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 538..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 310..324
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 538..570
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1145 AA; 121957 MW; 0D34E75D7A7538B6 CRC64;
MTISAQDIAG ALGLPRPTDE QASIIEHPPT PMLVVAGAGS GKTETMSARV VWLVANGHVE
PDRVLGLTFT RKAAAELASR VGARLAALRQ AGIWSPTNPA GATDPITASA TVSTYHAYAG
RIVSEHALRL GREPESRLLS EAASWQFANE AVASYDGPLE EIGRAEATIT NAVVDLSAEM
AEHLVTPDRL AEWLDAFEAG MARIEAGGRE LFAESKTMRR DLHGRRMLVP VLEAYERLKV
ARGALDFADQ MSLAARVASR FPVVGQIERA RFGAVLLDEF QDTSVAQLEL LRALFTESGG
GGRAVGGGAG EERAGKDRDG GDGVAGGRVC EGREGATGTH CVTAVGDPNQ SIYGWRGASA
ATLAQFPAAF GAARDAVLPL TQTWRNDARI LAAANAVAAP LRREAAVPVA PLTAAPSAAA
GRIEVARPET IEDEATQVAH WMAAQLRGGA RSGAVLCRKR SQFGPVVEAL AQAGLPYEVV
GLGGLLTTPE VQDLVALLTV AHDAARGDRL MRLLTGPKAR LGAADLDRFY AWARSSQQIQ
PRREAQRPLQ TQSPPQTQRA SRTPSAPEVQ RLNEDQRRPA DLSDGAADRV SLVEALVELD
RLVDSARVAT GISEAGLTRL HELAEAVGAI RRLSGLGLAE LVDEAERALG LDIEVLARPA
FGPAAARAHL DAFADIAANF SMSSDRPTLG GFLAWLDAAL EEERGLDRGN LEVAPDAVHV
LTVHAAKGLE WDAVAVPGLV EATFPVHTAT TSTWSAEAER WVVGQPSDGA WTRGIATLPY
DLRGDRLALP DLDWRSAPDA KELENRRKRF RSDAGARGID EERRLAYVAL TRARHRLLLT
APVWSTQGSP RVVSRFVTEL FDDVPDALAR GPWTPEPDPE ARNPRLSEPI TALWPVQPVI
PTPIAAAAAA VSGARTHTAD SDPGEVLGDP ELAVLLAERR ARQQPTSAAA PRAAHHSAAD
LVAAATDLEA FRLARRRPMP TRPAESTRQG TAFHAWVERH YRRAALLEPD DLPGSADEGG
AQSLDDLTAT FLASPWAGRE PLEVELAVET VIGGVAVRGR IDAVFRDGDG FVIVDWKSGH
APNGADADAK AVQLALYRLA YARLRDVPPS VVRAAFYYAA TGESVFPPLP SDAELVELAE
HASRE
//
