ID   A0A078AHQ5_STYLE        Unreviewed;       671 AA.
AC   A0A078AHQ5;
DT   29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT   29-OCT-2014, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   RecName: Full=S-formylglutathione hydrolase {ECO:0000256|ARBA:ARBA00016774};
DE            EC=3.1.2.12 {ECO:0000256|ARBA:ARBA00012479};
GN   Name=Contig192.g232 {ECO:0000313|EMBL:CDW81391.1};
GN   ORFNames=STYLEM_10407 {ECO:0000313|EMBL:CDW81391.1};
OS   Stylonychia lemnae (Ciliate).
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC   Stichotrichia; Sporadotrichida; Oxytrichidae; Stylonychinae; Stylonychia.
OX   NCBI_TaxID=5949 {ECO:0000313|EMBL:CDW81391.1, ECO:0000313|Proteomes:UP000039865};
RN   [1] {ECO:0000313|EMBL:CDW81391.1, ECO:0000313|Proteomes:UP000039865}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=130c {ECO:0000313|EMBL:CDW81391.1,
RC   ECO:0000313|Proteomes:UP000039865};
RA   Swart Estienne;
RL   Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the esterase D family.
CC       {ECO:0000256|ARBA:ARBA00005622}.
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DR   EMBL; CCKQ01009885; CDW81391.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A078AHQ5; -.
DR   EnsemblProtists; CDW81391; CDW81391; STYLEM_10407.
DR   InParanoid; A0A078AHQ5; -.
DR   OMA; DCIFCQS; -.
DR   OrthoDB; 451143at2759; -.
DR   Proteomes; UP000039865; Unassembled WGS sequence.
DR   GO; GO:0051903; F:S-(hydroxymethyl)glutathione dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0018738; F:S-formylglutathione hydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006069; P:ethanol oxidation; IEA:InterPro.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR   CDD; cd08300; alcohol_DH_class_III; 1.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013149; ADH-like_C.
DR   InterPro; IPR013154; ADH-like_N.
DR   InterPro; IPR014183; ADH_3.
DR   InterPro; IPR000801; Esterase-like.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR014186; S-formylglutathione_hydrol.
DR   NCBIfam; TIGR02821; fghA_ester_D; 1.
DR   PANTHER; PTHR43880; ALCOHOL DEHYDROGENASE; 1.
DR   PANTHER; PTHR43880:SF12; ALCOHOL DEHYDROGENASE CLASS-3; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   Pfam; PF00756; Esterase; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   SUPFAM; SSF50129; GroES-like; 2.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000039865}.
FT   DOMAIN          38..125
FT                   /note="Alcohol dehydrogenase-like N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF08240"
FT   DOMAIN          209..338
FT                   /note="Alcohol dehydrogenase-like C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00107"
FT   ACT_SITE        541
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT   ACT_SITE        617
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
FT   ACT_SITE        649
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR614186-1"
SQ   SEQUENCE   671 AA;  73936 MW;  ACBA119C745A263A CRC64;
     MESSSTQGQV IKCKAAVAWE ANKPLDICEI EVAPPQKGEI RVRVVANALC HTDIYTLDGH
     DPEGLFPCIL GHEATAIVES VGEGVTSVQV GDTVIPCYTP QCMERDCVFC MSQKTNLCPK
     IRATQGKGVM PDGTTRFSKD GKPIYHFMGC STFSEYTVIA EISAAKINPS ADLNKVCMLG
     CGVSTGWGAA MVNPEVKPGT TVAVWGLGAV GLSVIQAAKL QGAGKIYGID VNTDKFDHAK
     KLGADECMNP LEQDSKDWLL GKEKWGINYT YDCTGNVAVM RTALEAAHRG FGESCIIGVA
     AAGKEIQTRP FQLVTGRQWK GTAFGGWKSR EDVPKLVNKV IVGELNIDDF ITHTFESLED
     VNQSIDILHS GKCLRAVVKI SQQEQRPSLK VKILSSLKYH GGVLKTVSHW SSVNNCEMKF
     MIYLPNETIK EQRGKPYPAL YFLAGLTSNH ENAPIKSHFG SFAKKHGIAM IFPDTSPRGI
     EIEGIRENWW FGEGAGYYVD ATNEKWSKHF NMYSYITKEL PELVSDHFHV DKNKSSITGF
     SMGGMGALAL YLRNPGLYQS VSAFSPISHP TNSEWGKNAF EHYLGSVDAG KSYDPSLLIA
     EYDGPKTKIL FDQGSSDKFL QNLQPETFLN AAHKAGIDVQ YNFREGYSHD YFYVASFIEN
     HIEFHSRYLN A
//