ID A0A078J8S0_BRANA Unreviewed; 507 AA.
AC A0A078J8S0;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
DE Flags: Fragment;
GN Name=BnaC06g40900D {ECO:0000313|EMBL:CDY59895.1};
GN ORFNames=GSBRNA2T00027872001 {ECO:0000313|EMBL:CDY59895.1};
OS Brassica napus (Rape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708 {ECO:0000313|EMBL:CDY59895.1, ECO:0000313|Proteomes:UP000028999};
RN [1] {ECO:0000313|EMBL:CDY59895.1, ECO:0000313|Proteomes:UP000028999}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Darmor-bzh {ECO:0000313|Proteomes:UP000028999};
RX PubMed=25146293; DOI=10.1126/science.1253435;
RA Chalhoub B., Denoeud F., Liu S., Parkin I.A., Tang H., Wang X., Chiquet J.,
RA Belcram H., Tong C., Samans B., Correa M., Da Silva C., Just J.,
RA Falentin C., Koh C.S., Le Clainche I., Bernard M., Bento P., Noel B.,
RA Labadie K., Alberti A., Charles M., Arnaud D., Guo H., Daviaud C.,
RA Alamery S., Jabbari K., Zhao M., Edger P.P., Chelaifa H., Tack D.,
RA Lassalle G., Mestiri I., Schnel N., Le Paslier M.C., Fan G., Renault V.,
RA Bayer P.E., Golicz A.A., Manoli S., Lee T.H., Thi V.H., Chalabi S., Hu Q.,
RA Fan C., Tollenaere R., Lu Y., Battail C., Shen J., Sidebottom C.H.,
RA Wang X., Canaguier A., Chauveau A., Berard A., Deniot G., Guan M., Liu Z.,
RA Sun F., Lim Y.P., Lyons E., Town C.D., Bancroft I., Wang X., Meng J.,
RA Ma J., Pires J.C., King G.J., Brunel D., Delourme R., Renard M., Aury J.M.,
RA Adams K.L., Batley J., Snowdon R.J., Tost J., Edwards D., Zhou Y., Hua W.,
RA Sharpe A.G., Paterson A.H., Guan C., Wincker P.;
RT "Plant genetics. Early allopolyploid evolution in the post-Neolithic
RT Brassica napus oilseed genome.";
RL Science 345:950-953(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; LK033818; CDY59895.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A078J8S0; -.
DR STRING; 3708.A0A078J8S0; -.
DR PaxDb; 3708-A0A078J8S0; -.
DR EnsemblPlants; CDY59895; CDY59895; GSBRNA2T00027872001.
DR Gramene; CDY59895; CDY59895; GSBRNA2T00027872001.
DR OMA; WQKILRA; -.
DR Proteomes; UP000028999; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF41; ENDOGLUCANASE 5; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000028999};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 415..495
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
FT ACT_SITE 299
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:CDY59895.1"
SQ SEQUENCE 507 AA; 56605 MW; D8F67BD186832F11 CRC64;
TLWSIRWVTD YFIKAHPQPN VLWGQVGDGQ SDHYCWQRAE DMTTSRTAYK LDQYHPGSDL
AGETAAAFAA PSLAFKPYNS SYSTILLSHA KELFSFADKY RGLYTDSIPN AKAFYMSSGY
SDELLWAVAW LHRATGDQYY LKYTIDNAGY MGGTGWGMKE FSWDNKYAGV QVLLSKVLLE
GKGGPYTSTL KQYQMKADYF ACACLKKNGG YNIQTTPGGL MYVREWNNLQ YSSAAAFLLA
VYSDHLSAAN AKLNCPDGSV QPQALLDFAR SQADYILGKN RQGMSYLVGY GPKYPIRVHH
RGASVPSIFV QRSSVSCVQG FDSWYRRSQA DPNVIYGALV GGPDQNDNYS DDRTNYEQSE
PTLSGTAPLV GLFAKLSVGS YGGGYSKPYQ TTKQQATSYK ATPTTYTPKQ SGAPIEFLHS
ITANWMAGNT RYYRHKVIIK NSSQKQISDL KLKIEDLSGP IWGLIPTGQK NTYQLPRWQK
ILRAEQTHDF VYVQGGPQAK VSVLSYY
//
