ID A0A085EMA0_9FLAO Unreviewed; 470 AA.
AC A0A085EMA0;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=RNA methyltransferase {ECO:0000313|EMBL:KFC60345.1};
GN ORFNames=FEM08_08220 {ECO:0000313|EMBL:KFC60345.1};
OS Flavobacterium gilvum.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1492737 {ECO:0000313|EMBL:KFC60345.1, ECO:0000313|Proteomes:UP000028636};
RN [1] {ECO:0000313|EMBL:KFC60345.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EM1308 {ECO:0000313|EMBL:KFC60345.1};
RA Shin S.-K., Yi H.;
RT "Genome Sequence of Flavobacterium sp. EM1308.";
RL Submitted (MAY-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFC60345.1}.
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DR EMBL; JNCP01000020; KFC60345.1; -; Genomic_DNA.
DR RefSeq; WP_035634746.1; NZ_JNCP01000020.1.
DR AlphaFoldDB; A0A085EMA0; -.
DR STRING; 1492737.EM308_09905; -.
DR KEGG; fgl:EM308_09905; -.
DR PATRIC; fig|1492737.3.peg.813; -.
DR eggNOG; COG2265; Bacteria.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000028636; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 3..64
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 428
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 428
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 302
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 331
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 352
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 401
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 470 AA; 53985 MW; C49320BD171D4B74 CRC64;
MARKNTDKVV FHQIKVLDAG AKGVSVAKAP DGKVIFIPNV VPGDVVDVQT FKKRKAYYEG
KAVHFHQLSE HRVDPICEHF GVCGGCKWQN MNYDQQLFFK HNEVKNHLQR IGKIELPEFE
PILGSEKKFF YRNKMEFSFS NSRWLTEAEI GSEENLGNRN ALGFHIPKMW DKILDINKCH
LQEDPSNAIR NEVRAFANQN SLTFFNPRAH EGLLRTLMLR TASTGEIMVL IQFFEDDKAN
RELLLDHLYE KFPQITSLQY VVNNKANDTL YDTDIKLYKG RDYILEEMEG LKFSINAKSF
YQTNSEQAYE LYKITRDFAG LTGNEIVYDL YTGTGTIAQF VSKKAKKVIG VESVPDAIKD
AKANAERNNI DNCEFFVGDM KVVFNDSFIA QHGHPEVIIT DPPRDGMHKD VIEQIMKIAP
EKVVYVSCNS ATQARDLALM DEKYKVTRVR PVDMFPQTHH VENVVLLERR
//
