ID A0A085L9C7_9FIRM Unreviewed; 1072 AA.
AC A0A085L9C7;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Type I restriction enzyme endonuclease subunit {ECO:0000256|RuleBase:RU364115};
DE Short=R protein {ECO:0000256|RuleBase:RU364115};
DE EC=3.1.21.3 {ECO:0000256|RuleBase:RU364115};
DE AltName: Full=Type-1 restriction enzyme R protein {ECO:0000256|RuleBase:RU364115};
GN ORFNames=DK28_0207520 {ECO:0000313|EMBL:KFD41573.1};
OS Peptococcaceae bacterium SCADC1_2_3.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae.
OX NCBI_TaxID=1487582 {ECO:0000313|EMBL:KFD41573.1, ECO:0000313|Proteomes:UP000027084};
RN [1] {ECO:0000313|EMBL:KFD41573.1, ECO:0000313|Proteomes:UP000027084}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCADC1_2_3 {ECO:0000313|EMBL:KFD41573.1};
RA Tan B.F., Charchuk R., Li C., Nesbo C., Abu-Laban N., Foght J.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KFD41573.1, ECO:0000313|Proteomes:UP000027084}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCADC1_2_3 {ECO:0000313|EMBL:KFD41573.1};
RA Tan B., Charchuk R., Li C., Nesbo C., Abu-Laban N., Foght J.;
RT "Draft genome sequence of an unculturable Firmicutes affiliated with
RT Peptococcaceae sorted using a microfluidic device from methanogenic alkane-
RT degrading cultures.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Subunit R is required for both nuclease and ATPase
CC activities, but not for modification. {ECO:0000256|RuleBase:RU364115}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851,
CC ECO:0000256|RuleBase:RU364115};
CC -!- SUBUNIT: The type I restriction/modification system is composed of
CC three polypeptides R, M and S. {ECO:0000256|ARBA:ARBA00011296,
CC ECO:0000256|RuleBase:RU364115}.
CC -!- SIMILARITY: Belongs to the HsdR family. {ECO:0000256|ARBA:ARBA00008598,
CC ECO:0000256|RuleBase:RU364115}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFD41573.1}.
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DR EMBL; JJNX02000028; KFD41573.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A085L9C7; -.
DR REBASE; 95181; PspSCADCORF207535P.
DR Proteomes; UP000027084; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd22332; HsdR_N; 1.
DR CDD; cd18800; SF2_C_EcoR124I-like; 1.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR004473; Restrct_endonuc_typeI_HsdR.
DR InterPro; IPR040980; SWI2_SNF2.
DR InterPro; IPR021810; T1RH-like_C.
DR NCBIfam; TIGR00348; hsdR; 1.
DR PANTHER; PTHR30195:SF15; TYPE I RESTRICTION ENYME HINDI ENDONUCLEASE SUBUNIT; 1.
DR PANTHER; PTHR30195; TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR Pfam; PF11867; T1RH-like_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364115};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU364115};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Helicase {ECO:0000313|EMBL:KFD41573.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU364115};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU364115};
KW Reference proteome {ECO:0000313|Proteomes:UP000027084};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747,
KW ECO:0000256|RuleBase:RU364115}.
FT DOMAIN 262..459
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|SMART:SM00487"
SQ SEQUENCE 1072 AA; 123726 MW; 2F29C00707A2F6D7 CRC64;
MIYFNEDTLV QQITADYLRD QLGWDSVYAY NQETFGSEGL LGRNSDREIV LTRYLRQALE
KLNPNLPQTA YDEAVRQVVD YSHSQSLPAN NFDKYQLFKN GVLVSFQGAH GEIKKERLKI
FDFDDATHNH FLAVRELWVQ GDIYRRRIDI AGFVNGIPLL FIECKNIHKD LKNAYEKNLA
DYKDTIPHFF HHNAIIMLAN GEKAKIGTIT AGYEHFHEWK RLSESDPGVV DMETLLKGVC
HKKNFMDIFE NFILYDDSTG NRIKVLAKNH QYLGVNKVLK TLNNPGRDRG KLGVFWHTQG
AGKSYSMVFF TTKVHRKIGG NYTFLICTDR DDLDTQIYKT FAGCSVVDND KDPCRPTSGR
HLADLMAHHK AYLFTTIQKF NQEVDPNEGY TKRDDIIVIT DEAHRTQYGT LALNMRNALP
NANFIGFTGT PLFKDDKITI RVFGDYVSTY DFQRAVEDKA TVPLYYDSRG ETLGVATSDI
NERIAEKLEQ IEIDDIDVSQ RLEKELKRDY HIITAEKRLN QIAKDFVEHY TTAWETGKAM
FVCIDKLTCV RMYELIKQYW EQKAEEIEDA WKLSAGEEKD YLTNKLIWMK ETRMAVIVSE
EQGEVDKFRK WGLDIIPHRR IIKNGFELPD GTRIGVDSAF TNEEHPFRIA IVCAMWMTGF
NVPSLANLYL DKPLKAHTLM QAIARANRVY KDKNNGLIVD YGGILKNLRK ALATFTGTGD
TSRPGGIGPD GLDPTKTKEE LLEDLKQTIG LVAGFLNERK ASLEAIINAS GFERNKAIVA
AKEAVNENDE TRKKFEVMCR EVFIKFKACL TIPGVNAYRN QYNAINIVYK SLQKDRDQAN
ISDIIKQLHE VVDESIAIVP HKAGEPDKPY DISKIDFNRL RAEFEHVRTK NTIVQSLKSV
IEKKLERLIQ RNPLRTNFQE HYEKIVEEYN KEKDRQNIEA TFEALLKFVE ELDEEESRAV
REGLNEETLA VYDLLLKPDL IPKEIQRIKK VARELLETLK EKLKNLYSWQ DKEPTRDEVK
VLIRNFLWDE KTGLPVEYYT QSEVDKKAEA VFCHVYRAYP TVPSPYYATI EN
//
