ID A0A087E8D9_9BIFI Unreviewed; 795 AA.
AC A0A087E8D9;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=CRISPR-associated protein Cas3 {ECO:0000313|EMBL:KFJ04040.1};
GN ORFNames=BISU_1077 {ECO:0000313|EMBL:KFJ04040.1};
OS Bifidobacterium subtile.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=77635 {ECO:0000313|EMBL:KFJ04040.1, ECO:0000313|Proteomes:UP000029055};
RN [1] {ECO:0000313|EMBL:KFJ04040.1, ECO:0000313|Proteomes:UP000029055}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 11597 {ECO:0000313|EMBL:KFJ04040.1,
RC ECO:0000313|Proteomes:UP000029055};
RA Ventura M., Milani C., Lugli G.A.;
RT "Genomics of Bifidobacteria.";
RL Submitted (MAR-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFJ04040.1}.
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DR EMBL; JGZR01000005; KFJ04040.1; -; Genomic_DNA.
DR RefSeq; WP_024463316.1; NZ_JGZR01000005.1.
DR AlphaFoldDB; A0A087E8D9; -.
DR STRING; 77635.BISU_1077; -.
DR eggNOG; COG1203; Bacteria.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000029055; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF16; CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000029055}.
FT DOMAIN 10..199
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 261..443
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 468..633
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 795 AA; 90015 MW; 8AB34D1DEDF4746D CRC64;
MPELYARKDD EGSVQTLFDH LHNTARLAGE FEDEFGETAQ TAAIHHDEGK AKEDFQQYLF
ADDAKRGSVI HAYQGAFTIS EIPAASAAEK LTQEILELDI ASHHGDLPDC IAPSGDESFF
DKMSEANKGE EKYHYQEVKE NIPELELDTD ARFERSVKET QILLERLMHA GFYKQESLYF
SLGQYVKYIF SRLVDADRLD AGNFSSHQQL ASHKPDWQQL VDRFEKNIGA FDSTSPINKI
RSEISEACFN AADRPAGIYK LAVPTGGGKT LASLRFALHH ALQTQKSHII YVSPYLTITT
QTTKSFRDIL DLPKESDILL EHYSSVMNSD DDDDGKEDGS TINRRKLAAE RWDNPIIVTT
MVQFLETVMS SHGTKLRKFH NMANSVIVFD EIQSIPVNAI NLFNEVVSFL SKILNCTILL
CSATQPLLDK TDRHNLLLSE NPDLIEDSDK YAAALKRTNI VPIPQEKDLD ELADFVMERA
KENGNCLAIV NLKSEARKLY QHLEELNADG EFALVHLSTS MCGKHRANEL KKMIKLLESH
QPIICVSTQL IEAGVDISFA CVVRAMAGLD SVLQSAGRCN RNGESKEAKN VYVVSIKNER
GLEKLKDIKL GKEITNRLVQ DHPGVDLLSD DMLNEFYEFY FAPRQAEMDY PIDSSHNIYT
MLSSNEYDRN RYMDRTSKRY GHQLAQAFRT ASDQFHVIPN LTKDVLVPYA KDKNDTEFSD
ILDAFEKGDT KTKIRSLRLL QDYSVSLFDY EIEKLSEAHA ISVIDSEFGI SLLDKQYYKS
EYGVVLETEM SPLFA
//
