ID A0A094CGZ1_9PEZI Unreviewed; 1124 AA.
AC A0A094CGZ1;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=leucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013164};
DE EC=6.1.1.4 {ECO:0000256|ARBA:ARBA00013164};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00030520};
GN ORFNames=V493_07087 {ECO:0000313|EMBL:KFY21817.1};
OS Pseudogymnoascus sp. VKM F-4281 (FW-2241).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=1420906 {ECO:0000313|EMBL:KFY21817.1, ECO:0000313|Proteomes:UP000029327};
RN [1] {ECO:0000313|EMBL:KFY21817.1, ECO:0000313|Proteomes:UP000029327}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VKM F-4281 (FW-2241) {ECO:0000313|Proteomes:UP000029327};
RA Leushkin E.V., Logacheva M.D., Penin A.A., Sutormin R.A., Gerasimov E.S.,
RA Kochkina G.A., Ivanushkina N.E., Vasilenko O.V., Kondrashov A.S.,
RA Ozerskaya S.M.;
RT "Population genomics of a fungus Geomyces pannorum provides evidence of
RT horizontal gene transfer but not of sexual reproduction.";
RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KFY21817.1}.
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DR EMBL; JPJV01002520; KFY21817.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A094CGZ1; -.
DR STRING; 1420906.A0A094CGZ1; -.
DR HOGENOM; CLU_004174_1_1_1; -.
DR OrthoDB; 5472610at2759; -.
DR Proteomes; UP000029327; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07959; Anticodon_Ia_Leu_AEc; 1.
DR CDD; cd00812; LeuRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR004493; Leu-tRNA-synth_Ia_arc/euk.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00395; leuS_arch; 1.
DR PANTHER; PTHR45794:SF1; LEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR45794; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000029327}.
FT DOMAIN 66..134
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 222..798
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 837..966
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1124 AA; 126112 MW; 617B38FD1B834DC8 CRC64;
MAASDAAAKA LDDLHLSKTK ELKGTEKRDS LIAVEKKYQK KWQEDAVFQP NAPSIAEIPL
NALSAKELRE KHPKFFGTMA YPYMNGTLHA GHSFSVSKIE FTAGWARMQG KRALFPMGFH
CTGMPIKACA DKLINEIKLF GKDFSGYSED DAASDKLKDA GPPAPTSARE DVTKFTAKKG
KAASKVVKMK YQFQIMRAQG IPNEQIHEFA DPAHWLEFFP PLCKQDLTNF GCRIDWRRSF
VTTDANPYYD SFIRWQMNRL RELKKIKYGK RYTIYSPKDG QPCMDHDRSE GEAVGPQEYT
ALKLKVLEWA PEAKKAVADK LPQGADAFFV PATLRPETMY GQNCCFVGPK ITYGVYKVSE
KEYYIITERA ARNMAYQGVF PENGVYEKVA EVTGADCVGT LVNAPLSVHK EGVRILPMDS
VLPTKGTGVV TCVPSDSPDD FATITDLAKK AEYYGIKKEW AELEIVPIID TPSFGNLTAP
FLVKKLKIAS PKDTKQLEEA KELAYKEGYY QGTMCYGQFT GEKVEAAKPK VKQQLFDSGD
AFAYSEPEKR VVSRSGDDCC VALMDQWYLD YGEDSWRKVA LKHVDNADGN GLETYSQETK
NGFEGVLNWL NQWACARSFG LGSKLPWDPQ FLVESLSDST IYMAYYTVAH LLHGDIFGKT
PGLLKDVKPE QMTDEVWDYI FARREVSEDV LQSKIALEGL ETMRREFEYW YPLDLRVSGK
DLIPNHLTFF IYIHIALFPP EYWPKGVRAN GHLTLNGEKM SKSTGNFMTL EEMVKKYGAD
ASRIALADAG DGVADANFEE DVADNNVLRL FTLREWIEEQ VKEQDSLRTG EKNDFLDRLF
DNEMNAIARE AKAHFDDTNY KLALKSGLYD FIGARDFYRE ASTAAGIKMH KDLVFKYIEL
QALLLTVIAP HWSEYIWLEV LNKPESVQQA LFPEIPAPDA SLSAMREYVR TTASNITSAE
AAQQKKKAKG KDISFDPKLP KKLTIFAAKN FPSWQEKYID LVREVFDPAT KAFNDKELTP
RISKMGEMKK AMPFVQGLKK RLTGGEKPET VFDRKLAFDE SKTLLQMVPG LKKAAGLKIV
DIVEVAEGGA TGVVLGGGEV ASLPQPAAGA VPGVPTFHFE NVEE
//
