ID   A0A099JH77_9MICO        Unreviewed;       442 AA.
AC   A0A099JH77;
DT   07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT   07-JAN-2015, sequence version 1.
DT   27-MAR-2024, entry version 37.
DE   SubName: Full=O-acetylhomoserine (Thiol)-lyase {ECO:0000313|EMBL:MBB5640712.1};
DE   SubName: Full=O-acetylhomoserine aminocarboxypropyltransferase {ECO:0000313|EMBL:KGJ77551.1};
DE            EC=2.5.1.49 {ECO:0000313|EMBL:KGJ77551.1, ECO:0000313|EMBL:MBB5640712.1};
GN   ORFNames=BJ997_001260 {ECO:0000313|EMBL:MBB5640712.1}, GY21_07605
GN   {ECO:0000313|EMBL:KGJ77551.1};
OS   Cryobacterium roopkundense.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Cryobacterium.
OX   NCBI_TaxID=1001240 {ECO:0000313|EMBL:KGJ77551.1, ECO:0000313|Proteomes:UP000029864};
RN   [1] {ECO:0000313|EMBL:KGJ77551.1, ECO:0000313|Proteomes:UP000029864}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RuG17 {ECO:0000313|EMBL:KGJ77551.1,
RC   ECO:0000313|Proteomes:UP000029864};
RA   Sisinthy S.;
RL   Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:MBB5640712.1, ECO:0000313|Proteomes:UP000561726}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21065 {ECO:0000313|EMBL:MBB5640712.1,
RC   ECO:0000313|Proteomes:UP000561726};
RA   Klenk H.-P.;
RT   "Sequencing the genomes of 1000 actinobacteria strains.";
RL   Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|RuleBase:RU362118};
CC   -!- SIMILARITY: Belongs to the trans-sulfuration enzymes family.
CC       {ECO:0000256|ARBA:ARBA00009077, ECO:0000256|RuleBase:RU362118}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KGJ77551.1}.
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DR   EMBL; JPXF01000024; KGJ77551.1; -; Genomic_DNA.
DR   EMBL; JACHBQ010000001; MBB5640712.1; -; Genomic_DNA.
DR   RefSeq; WP_052542119.1; NZ_JPXF01000024.1.
DR   AlphaFoldDB; A0A099JH77; -.
DR   STRING; 1001240.GY21_07605; -.
DR   eggNOG; COG2873; Bacteria.
DR   OrthoDB; 9780685at2; -.
DR   Proteomes; UP000029864; Unassembled WGS sequence.
DR   Proteomes; UP000561726; Unassembled WGS sequence.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0003961; F:O-acetylhomoserine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0019346; P:transsulfuration; IEA:InterPro.
DR   CDD; cd00614; CGS_like; 1.
DR   Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR000277; Cys/Met-Metab_PyrdxlP-dep_enz.
DR   InterPro; IPR006235; OAc-hSer/O-AcSer_sulfhydrylase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR43797; HOMOCYSTEINE/CYSTEINE SYNTHASE; 1.
DR   PANTHER; PTHR43797:SF2; HOMOCYSTEINE_CYSTEINE SYNTHASE; 1.
DR   Pfam; PF01053; Cys_Met_Meta_PP; 1.
DR   PIRSF; PIRSF001434; CGS; 1.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR   PROSITE; PS00868; CYS_MET_METAB_PP; 1.
PE   3: Inferred from homology;
KW   Lyase {ECO:0000313|EMBL:MBB5640712.1};
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898,
KW   ECO:0000256|PIRSR:PIRSR001434-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000029864};
KW   Transferase {ECO:0000313|EMBL:KGJ77551.1}.
FT   MOD_RES         220
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001434-2"
SQ   SEQUENCE   442 AA;  47636 MW;  EBCD00A989E3B1C8 CRC64;
     MPRSDVAAPI TDWTEFSFDT RQVHVGEYPD QNRGARIPPL TLSAGYVFDS FDDGAARFNG
     DSREPIYSRQ GNPTNAVAEQ RLASLEGGSA AVVVSSGQAA ITAALFALAE SGEHIVSTAS
     IYGGTRILLG RSFRRFGVSV DYVWDTDDDA AWDASIRPET KAIFTETIPN PRNDVVDIAR
     IAAVARRHGI PLVVDNTVAT PYLIRPFEHG ADIVVHSTTK FLSGHGAGVS GAVIDGGTFD
     WAASSRRYPL LTDSLRRGLP SLLERFGPAA YAQYVREAVV NDIGPSLSPF NGFLLHQGIE
     TLSLRMERHV DNAIAIAHWL EEQPEVEVVD YAGLESSPLH DIAQHYYNGR TGSVFAVTVK
     GGSDGARAFT NGLRIFSRMT GIGDTRSMVL HPLTTTHVCF ADDLNARLGI TAGMLRLSVG
     IESVDDLISD LRGGLDRVAG IL
//