ID A0A099P606_PICKU Unreviewed; 2937 AA.
AC A0A099P606;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=General transcription and DNA repair factor IIH helicase subunit XPD {ECO:0000256|ARBA:ARBA00014344};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN ORFNames=JL09_g1434 {ECO:0000313|EMBL:KGK39416.1};
OS Pichia kudriavzevii (Yeast) (Issatchenkia orientalis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Pichia.
OX NCBI_TaxID=4909 {ECO:0000313|EMBL:KGK39416.1, ECO:0000313|Proteomes:UP000029867};
RN [1] {ECO:0000313|Proteomes:UP000029867}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SD108 {ECO:0000313|Proteomes:UP000029867};
RX PubMed=25159171; DOI=10.1186/s12934-014-0121-4;
RA Xiao H., Shao Z., Jiang Y., Dole S., Zhao H.;
RT "Exploiting Issatchenkia orientalis SD108 for succinic acid production.";
RL Microb. Cell Fact. 13:121-121(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the helicase family. RAD3/XPD subfamily.
CC {ECO:0000256|ARBA:ARBA00009146}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGK39416.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; JQFK01000009; KGK39416.1; -; Genomic_DNA.
DR VEuPathDB; FungiDB:C5L36_0B09190; -.
DR VEuPathDB; FungiDB:C5L36_0B09200; -.
DR eggNOG; KOG0951; Eukaryota.
DR eggNOG; KOG1131; Eukaryota.
DR HOGENOM; CLU_000335_1_0_1; -.
DR Proteomes; UP000029867; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProt.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR CDD; cd18788; SF2_C_XPD; 1.
DR Gene3D; 1.10.275.40; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 1.10.30.20; Bacterial XPD DNA helicase, FeS cluster domain; 1.
DR Gene3D; 2.60.40.150; C2 domain; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 6.
DR Gene3D; 1.10.3380.10; Sec63 N-terminal domain-like domain; 2.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR006555; ATP-dep_Helicase_C.
DR InterPro; IPR041094; Brr2_helicase_PWI.
DR InterPro; IPR048863; BRR2_plug.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR010643; HBB.
DR InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR InterPro; IPR006554; Helicase-like_DEXD_c2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010614; RAD3-like_helicase_DEAD.
DR InterPro; IPR013020; Rad3/Chl1-like.
DR InterPro; IPR001945; RAD3/XPD.
DR InterPro; IPR004179; Sec63-dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR InterPro; IPR042493; XPD_DNA_FeS.
DR NCBIfam; TIGR00604; rad3; 1.
DR PANTHER; PTHR47961; DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED; 1.
DR PANTHER; PTHR47961:SF4; U5 SMALL NUCLEAR RIBONUCLEOPROTEIN HELICASE; 1.
DR Pfam; PF21188; BRR2_plug; 1.
DR Pfam; PF00270; DEAD; 2.
DR Pfam; PF06733; DEAD_2; 1.
DR Pfam; PF06777; HBB; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF13307; Helicase_C_2; 1.
DR Pfam; PF18149; Helicase_PWI; 1.
DR Pfam; PF02889; Sec63; 2.
DR PRINTS; PR00852; XRODRMPGMNTD.
DR SMART; SM00382; AAA; 2.
DR SMART; SM00487; DEXDc; 3.
DR SMART; SM00488; DEXDc2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00491; HELICc2; 1.
DR SMART; SM00973; Sec63; 2.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 5.
DR SUPFAM; SSF158702; Sec63 N-terminal domain-like; 2.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242}.
FT DOMAIN 504..688
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 722..932
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 1350..1526
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 2136..2414
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51193"
FT REGION 209..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2891..2913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..240
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2937 AA; 335645 MW; FFBB3457325E8B7E CRC64;
MAEDLGKYSY EEISNKVLQV DRRLFDDGSN NARFEDTNPH SLKGVISSKD FGSNIKTNRA
EDDKGKEELK NEIQTTLAAF EVSDRKNLKQ KSKTSSTARS LESLLDAGSI EALNYTPTTE
STMHTYDEII AWSNEKLDSD LPDDVIISFT DIIIEILKNE NSNSLEKKKQ LETALKQKID
DDDFRLVYSL CGKLIDYKQV LDVPDKDNDE LDIQLDSDDE NAEDGIEVTD SENDDDGSIS
SDVNDEQRDD SIFINQETDN PDDIVRLSLN DDQIPALSID EVDKYWITKQ LAKYQAQIDN
YKHAEISKSI YRILEQLVEK RENLKSFMHR LLEIPDFEIN DLYYLLVKNY KVIYYGIKLA
STEDKAEKDN IRALCSDLNS NKRTLSTVEA DDQVLNREKR QKTLTEGKIA SNYDNKYPRN
LNLKNLVFTQ GSKLMTVSKF KLPEGSFKRT KPSWEEINIP PPVKSQMGED EVLVEISELP
EWIQPVFPST EISTLNRIQS KVFPVAFNSD DNLLMCAPTG AGKTNVAMLT VLRTLSNYRT
TGGKFQLSRF KIVYIAPLKA LVQEQVREFD RRLSKYGITV NELTGDSNLT KHQIESTQIL
VTTPEKWDVI TRKNNDASYI NLVKLVIIDE IHLLHDERGP VLESIVARTM KNIDESRENS
VRFVGLSATL PNYKDVAQFL RVEPSGLFYF DASYRPCPLA QQFIGITEKK AFKKYEAMNE
ICYEKIVENI SEGNQVIIFV HSRKETIKTA KWLVNRLVEE DKLSTLFKFS KGVREILRSE
AENARNKGLK EVLLSGFGIH HAGMNRDDRQ TAEDLFAEGH IKVLVSTATL AWGVNLPAHT
VVIKGTSVYS PEKGTWVDLS PQDILQMLGR AGRPRYDTHG DGIIITSQDQ VKYYLAVLNQ
QLPIESQMYA KLADSINAEI VAGSIRSLKD CIDWMGHTYL YVRMLHSREV YYVGPQYDND
PELVERRKDL GYSTFLLLAK NGLVKYNFER DMVIPTSLGR IASHYYISYE NMQNFESQLK
PTLTKIELFR IFATSEEFKY IPVRREEKLE LQKLLEKAPI PVNEDVEDPL SKINILLQAY
ISGLRLDGFA LMADMIFISQ SAGRLFRALY DLSLDKKWSK LTRVLLSVCK MVENRMWPTS
TPLRQFPNIP STIVDSVERT LIPWKQYLSI NDPQTATKSL GAERFGNLGW ELLQKFPKVH
IDYNLQPITA SLVQIELLVT PNWKWDVQAH GYIETFVLLV DDCDGKRILY NDKFSVRKDY
INQIHRVTFS VPVSENLEPN YFVSLISEKW IGCESRIPVM LTKMILPKKF PAPTPLINLD
LIPVSELGIK EFTSVFSFDY FNKFQSQAFD SIYNSTDNVL FSCSKGNGKT TLALLAILNH
WRNEGGRAVY LNPHQSVVDN LLKHWKKKLS KLAGGKIINK LTGDINIDLQ ILAQSHLVLA
TPSDFEIISR RWQQRKSLQS IELLIADDIH TIGSGKEGVS YELVISRFRY IATNLEKNIR
FVLLGASIAS YRDIMDWVSI PKTNVFVFDA RERIYPVQVK FHYMDIHHNP SLFKCLIKPA
YNHIANLTAI KGDSTCLAFV PQRSDVLDLA KEFLRKVRTD GSNWLSGGED EVQPYIDRVS
DSTLKLCIRF GIGFYFEGMD TVEQTIVFSL FEAGILKVMI TTKSSSCVCP SANSVIILGT
KEYDAKEHGY YDYRINDILD MIGLARRDNG TDATAIVYTN SSKLEFYKRF ITLSLPFESH
LNNFINDVFI SDISIIKNRQ YALDWITYSL FYRRLQLNPS FYKLDDASED GLSGYLSELV
ENSLSKLQEA NLVELNLEDD KNSMEAEEED EYEKEEITPT ENGVISNYYN VDYATMEVFN
NLNENMRLKK ILEFLSLAAE FDSLPIRSGE SNLLFKLYNG VPLKWTLDVD FESPKFKTFI
LLQAYFSGFH LNADLKKDLE YILPTANRLL NAIVDILSSH GHLNALVAMD LCQMISQGIW
NTDNVLKQIP HFNDELIAKC KTYNVDTIYD LMELEDKDRE DLLSELNQQE IIKVAEFVNK
YPNLEVAYKI EGDVVAGESK TIEIEISRDE EVEGLSIASN AFPKKKFESW WIVMGNPKTN
ELYSIKKLAI TKENQTIKLN FAIPEAGEHD LFFIDDLPVI FPYPKIYPEQ YAYMCDIKKS
LDAGGNCILE MPSGTGKTVS LLSITVAYQM HYPEHRKIIY CSRTMSEIEK ALLELEKLMD
YRSKELGYIE NFRGLGLTSR KNLCLHPTIS KERKGIVVDE KCRRITNGQL KEKVNSGEAD
VEDLCSFHEK MYDIDPTNYV PPGIYPFEKL IDYCKTEGTC PYFTVRRMMQ YCNIIIYSYH
YLIDPKIAER VSKDLSGDSI VIFDEAHNID NVCIESLSMD ITDDVLKKAT KAAESLNKKV
EEVKKVDSQK LQDEYEKLVE GLRESNIVNP GEETFVANPV LSSDVLDEAI PGSIRRAEHF
ISFMKRLIEY LKTRMKVLHV IAETPTSFLQ HLKQLTFIDR KPLRFCSERL SLLVRTLEVS
DVEDYMALKD IATFASLVST YENGFTLILE PFETENATVP NPILRLSCLD ASIAIKPVFE
RFSSVIITSG TISPLDMYPK MLNFETVIQE SYSMTLDRRS FLPMIVSKGS DQVAISSRFE
IRNDPSVVRN YGTLVIEFSK TTPDGLVVFF PSYLYMESII SQWQTMGILD EVWKYKLILV
ETPDAQETSL ALETYRKACS NGRGAVLFSV ARGKVSEGID FDHHYGRTVL MIGIPFQYTE
SRILKARLEF LRDNYQIREN DFLSFDAMRH AAQCLGRVLR GKDDYGVMVL ADRRFSRRKG
QLPKWIAQAL LDADTNLSTD MAVASAKKFL RTSAQPIDPL DQESGVSVWT YEQLLQHQKK
QQGKLGYEEE NENGMDIDND NDNDSNPGNN VDVDVAMEDA NYNGNAADVD MKRVTVQ
//
