ID A0A099P7W0_PICKU Unreviewed; 490 AA.
AC A0A099P7W0;
DT 07-JAN-2015, integrated into UniProtKB/TrEMBL.
DT 07-JAN-2015, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Cytochrome b-c1 complex subunit 8 {ECO:0000256|RuleBase:RU368118};
DE AltName: Full=Complex III subunit 8 {ECO:0000256|RuleBase:RU368118};
GN ORFNames=JL09_g850 {ECO:0000313|EMBL:KGK40136.1};
OS Pichia kudriavzevii (Yeast) (Issatchenkia orientalis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Pichia.
OX NCBI_TaxID=4909 {ECO:0000313|EMBL:KGK40136.1, ECO:0000313|Proteomes:UP000029867};
RN [1] {ECO:0000313|Proteomes:UP000029867}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SD108 {ECO:0000313|Proteomes:UP000029867};
RX PubMed=25159171; DOI=10.1186/s12934-014-0121-4;
RA Xiao H., Shao Z., Jiang Y., Dole S., Zhao H.;
RT "Exploiting Issatchenkia orientalis SD108 for succinic acid production.";
RL Microb. Cell Fact. 13:121-121(2014).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a
CC multisubunit transmembrane complex that is part of the mitochondrial
CC electron transport chain which drives oxidative phosphorylation. The
CC complex plays an important role in the uptake of multiple carbon
CC sources present in different host niches.
CC {ECO:0000256|RuleBase:RU368118}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189;
CC Evidence={ECO:0000256|ARBA:ARBA00001702};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC Evidence={ECO:0000256|ARBA:ARBA00001702};
CC -!- SUBUNIT: Component of the ubiquinol-cytochrome c oxidoreductase
CC (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme
CC composed of 3 respiratory subunits cytochrome b, cytochrome c1 and
CC Rieske protein, 2 core protein subunits, and additional low-molecular
CC weight protein subunits. The complex exists as an obligatory dimer and
CC forms supercomplexes (SCs) in the inner mitochondrial membrane with
CC cytochrome c oxidase (complex IV, CIV).
CC {ECO:0000256|RuleBase:RU368118}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004167}. Mitochondrion
CC inner membrane {ECO:0000256|ARBA:ARBA00004434,
CC ECO:0000256|RuleBase:RU368118}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004434, ECO:0000256|RuleBase:RU368118}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. ZNG1
CC subfamily. {ECO:0000256|ARBA:ARBA00034320}.
CC -!- SIMILARITY: Belongs to the UQCRQ/QCR8 family.
CC {ECO:0000256|ARBA:ARBA00007668, ECO:0000256|RuleBase:RU368118}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGK40136.1}.
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DR EMBL; JQFK01000004; KGK40136.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A099P7W0; -.
DR VEuPathDB; FungiDB:C5L36_0E01700; -.
DR VEuPathDB; FungiDB:C5L36_0E01710; -.
DR eggNOG; KOG2743; Eukaryota.
DR eggNOG; KOG4116; Eukaryota.
DR HOGENOM; CLU_017452_6_0_1; -.
DR Proteomes; UP000029867; Unassembled WGS sequence.
DR GO; GO:0005750; C:mitochondrial respiratory chain complex III; IEA:UniProtKB-UniRule.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IEA:UniProtKB-UniRule.
DR CDD; cd03112; CobW-like; 1.
DR Gene3D; 3.30.1220.10; CobW-like, C-terminal domain; 1.
DR Gene3D; 1.20.5.210; Cytochrome b-c1 complex subunit 8; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR036627; CobW-likC_sf.
DR InterPro; IPR011629; CobW-like_C.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR004205; Cyt_bc1_su8.
DR InterPro; IPR036642; Cyt_bc1_su8_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR13748:SF31; COBW DOMAIN-CONTAINING PROTEIN 1-RELATED; 1.
DR PANTHER; PTHR13748; COBW-RELATED; 1.
DR Pfam; PF02492; cobW; 1.
DR Pfam; PF07683; CobW_C; 1.
DR Pfam; PF02939; UcrQ; 1.
DR SUPFAM; SSF90002; Hypothetical protein YjiA, C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF81508; Ubiquinone-binding protein QP-C of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase); 1.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|ARBA:ARBA00022982,
KW ECO:0000256|RuleBase:RU368118}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128,
KW ECO:0000256|RuleBase:RU368118};
KW Mitochondrion inner membrane {ECO:0000256|ARBA:ARBA00022792,
KW ECO:0000256|RuleBase:RU368118};
KW Respiratory chain {ECO:0000256|ARBA:ARBA00022660,
KW ECO:0000256|RuleBase:RU368118};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU368118}.
FT DOMAIN 45..251
FT /note="CobW/HypB/UreG nucleotide-binding"
FT /evidence="ECO:0000259|Pfam:PF02492"
FT DOMAIN 309..392
FT /note="CobW C-terminal"
FT /evidence="ECO:0000259|Pfam:PF07683"
SQ SEQUENCE 490 AA; 55620 MW; 8E70751D08508822 CRC64;
MEEELEYIPN LVENENEEIV FEKVKKEPID TTGTIIEEDK SKKVPVTILT GYLGSGKSTL
LEQIAKRGDR KLAVILNEFG DSIDIEKSLT VKDKENEVEE WLDLGNGCLC CTVKDNGVAA
IERLVAKRQG FDHIILETTG LADPGPITTM FWLDDGLLSN VYIDGVVTVL DAENIETNLS
DKDESHVHYA EEGTHGEDPH HNCDNVTVAH VQIALADVIL LNKIDKIEND PKRIKRIESL
VRDINANVPI YETKFGDISL DKILDLHAYE ARDVSSLVNT EKSTSGVHDH RMGTLSFNFK
KLVDIREFKK FEKFLQDLLW DDSDADLLRN NMEIHRTKGL VFIEDGDSYK VIQGVRKTYD
VIDGMQDILE IELDSSRLVL IGKYIDVSNL QQPTNYIKMG HPGAYMGWWG SMGSPKQKRI
TIHSVSPYAQ SPLHGSVNRA IFNSFRRFKS QVLYIALPFA IVWSVWTEAR DYNEYLYTKA
GREELERVNV
//
