ID A0A0B0PK51_GOSAR Unreviewed; 741 AA.
AC A0A0B0PK51;
DT 04-MAR-2015, integrated into UniProtKB/TrEMBL.
DT 04-MAR-2015, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=F383_07738 {ECO:0000313|EMBL:KHG23756.1};
OS Gossypium arboreum (Tree cotton) (Gossypium nanking).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=29729 {ECO:0000313|EMBL:KHG23756.1, ECO:0000313|Proteomes:UP000032142};
RN [1] {ECO:0000313|Proteomes:UP000032142}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. AKA8401 {ECO:0000313|Proteomes:UP000032142};
RA Mudge J., Ramaraj T., Lindquist I.E., Bharti A.K., Sundararajan A.,
RA Cameron C.T., Woodward J.E., May G.D., Brubaker C., Broadhvest J.,
RA Wilkins T.A.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; KN426197; KHG23756.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0B0PK51; -.
DR Proteomes; UP000032142; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF51; BETA-GALACTOSIDASE 5; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000032142};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..741
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002077561"
FT DOMAIN 35..339
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 362..418
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 471..538
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT DOMAIN 633..718
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 741 AA; 82977 MW; 85E70BBDC1D886AB CRC64;
METSSVSKLL IFFFSAFLAS SKLIQCSITY DKKAILINGQ RRILISGSIH YPRSTPEMWE
DLIKKAKDGG LDVIDTYVFW NGHEPSPGNY NFEGRYDLVR FIKTVQKLGL YVHLRIGPYI
CAEWNFGGFP VWLKYVPGIS FRTDNEPFKR AMQGFTQKIV QMMKNEKLFA SQGGPIILSQ
IENEYGPESR ALGAAGHAYI NWAAKMAVEL NTGVPWVMCK EDDAPDPVIN ACNGFYCDGF
SPNKPYKPTM WTEAWSGWFT EFGGPIHQRP VQDLAFGVAR FIQKGGSYVN YYMYHGGTNF
GRTAGGPFIT TSYDYDAPID EYGLIRQPKY DHLKELHRAI KLCEHALVSS DPTVTSLGTY
HQAHVFSSRQ GGCAAFLSNF HMKSAARVTF NNRHYDLPPW SISILPDCKN VAFNTALVGV
KTSRIQMLPT NTKMFPWEAY GEDISSLGAS SRITAPGLLE QMNVTRDNSD YLWYTTSVDI
SPSESFLRGG QKPTLNVDSA GHALHVFVNG QFSGSAYGTR ENRRFTYTGP VNLHAGTNHI
ALLSVAVGLP NDGLHFETWK TGIQSVLLHG LNQGKKDLTW QKWSYQAHIT TLKQVGLRGE
AMNLVSPHGA SSVEWIRGER IQGSLAARSR QSMTWYKTYF NAPGGNEPLA LDMRSMGKGQ
VWINGQSLGR YWMAYAKGNC GTCSYLGTFR TTKCQSGCDQ PTQRWYHVPR SWLKPTKNLL
VVFEELGGDV SKISLVRRSV L
//
