ID A0A0C9XXS1_9AGAR Unreviewed; 791 AA.
AC A0A0C9XXS1;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=tRNA ligase {ECO:0000256|PIRNR:PIRNR019634};
DE EC=6.5.1.3 {ECO:0000256|PIRNR:PIRNR019634};
GN ORFNames=K443DRAFT_300894 {ECO:0000313|EMBL:KIK09786.1};
OS Laccaria amethystina LaAM-08-1.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Agaricineae; Hydnangiaceae; Laccaria.
OX NCBI_TaxID=1095629 {ECO:0000313|EMBL:KIK09786.1, ECO:0000313|Proteomes:UP000054477};
RN [1] {ECO:0000313|EMBL:KIK09786.1, ECO:0000313|Proteomes:UP000054477}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LaAM-08-1 {ECO:0000313|EMBL:KIK09786.1,
RC ECO:0000313|Proteomes:UP000054477};
RG DOE Joint Genome Institute;
RA Kuo A., Kohler A., Nagy L.G., Floudas D., Copeland A., Barry K.W.,
RA Cichocki N., Veneault-Fourrey C., LaButti K., Lindquist E.A., Lipzen A.,
RA Lundell T., Morin E., Murat C., Sun H., Tunlid A., Henrissat B.,
RA Grigoriev I.V., Hibbett D.S., Martin F., Nordberg H.P., Cantor M.N.,
RA Hua S.X.;
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000054477}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LaAM-08-1 {ECO:0000313|Proteomes:UP000054477};
RG DOE Joint Genome Institute;
RG Mycorrhizal Genomics Consortium;
RA Kohler A., Kuo A., Nagy L.G., Floudas D., Copeland A., Barry K.W.,
RA Cichocki N., Veneault-Fourrey C., LaButti K., Lindquist E.A., Lipzen A.,
RA Lundell T., Morin E., Murat C., Riley R., Ohm R., Sun H., Tunlid A.,
RA Henrissat B., Grigoriev I.V., Hibbett D.S., Martin F.;
RT "Evolutionary Origins and Diversification of the Mycorrhizal Mutualists.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.;
CC EC=6.5.1.3; Evidence={ECO:0000256|PIRNR:PIRNR019634};
CC -!- SIMILARITY: Belongs to the TRL1 family.
CC {ECO:0000256|PIRNR:PIRNR019634}.
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DR EMBL; KN838537; KIK09786.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0C9XXS1; -.
DR STRING; 1095629.A0A0C9XXS1; -.
DR HOGENOM; CLU_010316_1_0_1; -.
DR OrthoDB; 1405617at2759; -.
DR Proteomes; UP000054477; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051730; F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0003972; F:RNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR019039; T4-Rnl1-like_N.
DR InterPro; IPR012387; Trl1_fun.
DR InterPro; IPR015966; tRNA_lig_kin_fungi.
DR InterPro; IPR015965; tRNA_lig_PDEase.
DR PANTHER; PTHR32004; TRNA LIGASE; 1.
DR PANTHER; PTHR32004:SF1; TRNA LIGASE; 1.
DR Pfam; PF09511; RNA_lig_T4_1; 1.
DR Pfam; PF08302; tRNA_lig_CPD; 1.
DR Pfam; PF08303; tRNA_lig_kinase; 1.
DR PIRSF; PIRSF019634; tRNA_lig_yeast; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR019634};
KW Reference proteome {ECO:0000313|Proteomes:UP000054477};
KW tRNA processing {ECO:0000256|PIRNR:PIRNR019634}.
FT DOMAIN 63..307
FT /note="T4 RNA ligase 1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09511"
FT DOMAIN 413..553
FT /note="tRNA ligase kinase"
FT /evidence="ECO:0000259|Pfam:PF08303"
FT DOMAIN 599..785
FT /note="tRNA ligase phosphodiesterase"
FT /evidence="ECO:0000259|Pfam:PF08302"
FT ACT_SITE 116
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR019634-50"
SQ SEQUENCE 791 AA; 87481 MW; 76FE8FF934BE298D CRC64;
MPPSFSQSDS ALISSLLSLS KKSPKLVKSS IYPAPASPDI SLRSWKMNEF KYYDIPSPFP
TLARGLFTFT ETEGGEERHR IVVRGYDKFF NIGEVPWTTW ASLESHTTAP YTLSLKSNGC
IIFIAALTPA KIVVTSKHSL GPVGNTTLSH AQAGENWLRK YFEKVGKSEE DLAKQLWENN
WTAIAELCDD SFEEHVLAYP PEKTGLHLHG LNVCTKDFHT LPHSVVDAFA EEWGFIKTAT
TTLNTIQEVR EFTDAVAKAG EWNGEAVEGF VVRTHVVEPL TGKTRTDKSP YAPGSSFFFK
VKFDEPYMMY RDWREVTKSL LSSKGAMKAS NLPKSKMKRA ETKVYVKWVI EEIKKHPEEF
AEYTKGKGIV RTREKFLAWL AEEGKAELKA VEEQTDKEVK EEEGKAKVSG KTVIVPIAIP
GCGKTAVSVA LAHIFGFGHT QSDDVHVKKA APIFVKNVMS LLQTHDVVIA DKNNHLRQHR
QSIRESTSKL PTPVRLLALN WSLDKPPSTI HRICADRVQS RGANHQTLRA DLSASKSHED
VVWMFINTTE ELASSEVDEC IEMELEDSLE EAVERAVDGV ARILGIPHPG EEKVKEGVEL
VKGYEPSSKK PDDPKKKAAE ARYYGLLPEV DLAGLLDAQL AGTEGGKFWD QLKKSGRVTK
RPHVTLVHKT ELPASKELWD RCAGLHKSNS PPLLRGTLGS VVWDGRVMAV SVDDVGVEEG
EGGEGLEGEA FVAGLGGDVR GRMHITVGTR DGNVAPVEAK GLVEGWRRGE AVKSVKLGDV
VFRGRVKALM N
//
