ID A0A0D3A6I7_BRAOL Unreviewed; 739 AA.
AC A0A0D3A6I7;
DT 29-APR-2015, integrated into UniProtKB/TrEMBL.
DT 29-APR-2015, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
OS Brassica oleracea var. oleracea.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=109376 {ECO:0000313|EnsemblPlants:Bo1g042930.1, ECO:0000313|Proteomes:UP000032141};
RN [1] {ECO:0000313|EnsemblPlants:Bo1g042930.1, ECO:0000313|Proteomes:UP000032141}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. TO1000 {ECO:0000313|Proteomes:UP000032141};
RX PubMed=24916971; DOI=10.1186/gb-2014-15-6-r77;
RA Parkin I.A., Koh C., Tang H., Robinson S.J., Kagale S., Clarke W.E.,
RA Town C.D., Nixon J., Krishnakumar V., Bidwell S.L., Denoeud F., Belcram H.,
RA Links M.G., Just J., Clarke C., Bender T., Huebert T., Mason A.S.,
RA Pires J.C., Barker G., Moore J., Walley P.G., Manoli S., Batley J.,
RA Edwards D., Nelson M.N., Wang X., Paterson A.H., King G., Bancroft I.,
RA Chalhoub B., Sharpe A.G.;
RT "Transcriptome and methylome profiling reveals relics of genome dominance
RT in the mesopolyploid Brassica oleracea.";
RL Genome Biol. 15:R77-R77(2014).
RN [2] {ECO:0000313|EnsemblPlants:Bo1g042930.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2015) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR AlphaFoldDB; A0A0D3A6I7; -.
DR STRING; 109376.A0A0D3A6I7; -.
DR EnsemblPlants; Bo1g042930.1; Bo1g042930.1; Bo1g042930.
DR Gramene; Bo1g042930.1; Bo1g042930.1; Bo1g042930.
DR eggNOG; KOG0496; Eukaryota.
DR HOGENOM; CLU_007853_4_0_1; -.
DR OMA; RGNCGNC; -.
DR Proteomes; UP000032141; Chromosome C1.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-KW.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF63; BETA-GALACTOSIDASE 12; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Apoplast {ECO:0000256|ARBA:ARBA00022523};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000032141};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..739
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002268187"
FT DOMAIN 36..338
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 362..417
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 471..533
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT DOMAIN 620..706
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 739 AA; 82411 MW; 86F309BDC3F9C413 CRC64;
MGLNFGNKAW VFLGIICCSL LICSVKAMVT YDHKAVIING QRRILLSGSI HYPRSTPEMW
PDLIQKAKDG GLDVIQTYVF WNGHEPSPGQ YYFEDRYDLV KFVKLVQQAG LYVHLRIGPY
VCAEWNFGGF PVWLKYVPGM AFRTDNEPFK AAMQKFTEKI VGMMKEEKLF ETQGGPIILS
QIENEYGPIE WEIGAPGKAY TKWAAQMAEG LSTGVPWIMC KQDDAPDSII NTCNGYYCEN
FKPNSDNKPK MWTENWTGWF SEFGGAVPYR PAEDIAFSVA RFIQNGGSFI NYYMYHGGTN
FDRTAGEFMA TSYDYDAPLD EYGLPREPKY SHLTKLHKVI KLCEPALVSV DPTVTSLGDK
QEAHVFKSKS SCAAFLSNYN TSSAARVSFG GATYDLPPWS VSILPNCKTE YYNTAKVQVR
TPSIQMKMVP TNTQLSWGSY SEEIPSPNYN GTFAKDGLVE QISITRDKTD YFWYLTDITI
SADEKFLKTG EDPLLTIGSA GHALSVFVNG QFVGTAYGSL EKPKLTFSQK IKLAAGVNKL
ALLSIAVGLP NVGLHYETRN TGVLGPVTLE GVNSGTWDMS KWKWSYKIGT KGEALSIHTV
TGSSTVEWKE GTLVAKKQPL TWYKSTFDTP AGNEPLALDM NTMGKGQVWI NGQNIGRHWP
AYKARGKCDR CSYAGTFNEK KCLSNCGEAS QRWYHVPRSW LKPSANLIVV LEEWGGDPNG
ISLVRRTSKK IASESIFSG
//
